In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods

Amador, Paula; Fernandes, Rúben; Duarte, Isabel; Brito, Luísa; Prudêncio, Cristina

doi:10.1007/s11274-010-0635-9

Cited by 8 publications

(4 citation statements)

References 36 publications

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“…In general these studies show an increasing concern for these matters, an increasing of antibiotic resistance genes among other environments not associated with healthcare, and uncontrolled use of antibiotics with prolonged exposure often resulting in bacterial resistance [ 32 – 38 ]. Interestingly, regarding this matter, members of the team have identified three new TEM variants, within water and foodborne enterobacterial pathogens (TEM-179, TEM-180, and recently TEM-201) but not within the clinic isolates [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal

Fernandes

Amador

Oliveira

et al. 2014

The Scientific World Journal

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Extended-spectrum β-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains. In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including Escherichia coli (67.9%), Klebsiella pneumoniae (30.6%), Klebsiella oxytoca (0.5%), Enterobacter aerogenes (0.5%), and Citrobacter freundii (0.5%). β-lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. TEM enzymes were among the most prevalent types (40.9%) followed by CTX-M (37.3%) and SHV (23.3%). Among our sample of 193 ESBL-producing strains 99.0% were resistant to the fourth-generation cephalosporin cefepime. Of the 193 isolates 81.3% presented transferable plasmids harboring bla ESBL genes. Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences. This study reports a high diversity of genetic patterns. Ten clusters were found for E. coli isolates and five clusters for K. pneumoniae strains by means of ERIC analysis. In conclusion, in this country, the most prevalent type is still the TEM-type, but CTX-M is growing rapidly.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal

Fernandes

Amador

Oliveira

et al. 2014

The Scientific World Journal

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“…In developing countries, the growing use of antibiotics in veterinary medicine, mainly by small poultry farmers, has been associated with increased levels of antimicrobial resistance in E. coli strains, in fact, meat and meat products are frequently linked to the prevalence of antimicrobial resistance genes (12). Since bacteria are highly prone to exchanging genetic material, these resistance genes can be transferred to other bacteria, particularly Enterobacteriaceae (13). The sale of RTE street food, despite representing an important source of income for many unemployed families, has often been associated with outbreaks of diarrheal diseases (14).…”

Section: Introductionmentioning

confidence: 99%

Multiplex-PCR for diagnosis of Entamoeba histolytica and Escherichia coli O157 in the patients of Wasit Province, Iraq

Ali¹,

Flaaeih²

2022

ijhs

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This study was carried out at Wasit Province / Iraq in cooperation with Fairouz Hospital/ unit of microbiology. Samples were collected from many places at Wasit province. It included 100 stool samples from Iraqi patients with diarrhea. The study was conducted during the period from October to December, 2021 to investigate the prevalence of intestinal parasites and Escherichia coli O157 bacteria. Only 65 stool sample (65%) were positive for Entamoeba histolytica and E.coli O157. Rate of infection in current study with intestinal parasites was 65% among diarrheal cases were that from those samples E.histolytica. The rate of E.histolytica infection was 65 (65%) samples, while only 14 E.coli O157 isolates (21.5%) were positive using PCR technique. The higher rate of parasitic infection 15 (23.1%) was recorded in the age group (1-10) years old, while for E.coli O157 infection the rate was 4 (6.1%) in the age group (1-10) years old. Regarding gender males for E.histolytica rate was 60% male and 40% females, for E.coli O157 rate was 50% to 50% in this study without significant differences.

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“…In fact, meat and meat products are frequently linked to the prevalence of antimicrobial resistance genes [13]. Since bacteria are highly prone to exchanging genetic material, these resistance genes can be transferred to other bacteria, particularly Enterobacteriaceae [14].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Escherichia coli from Water and Food Sold on the Streets of Maputo: Molecular Typing, Virulence Genes, and Antibiotic Resistance

Salamandane

Alves

Chambel

et al. 2022

Applied Microbiology

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The aim of this study was to investigate the pathogenic potential and antibiotic resistance of 59 Escherichia coli isolates from ready-to-eat (RTE) street food (n = 31) and drinking water (n = 28) sold in the city of Maputo, Mozambique. The isolates were characterized by XbaI subtyping analysis via pulsed field gel electrophoresis. Multiplex PCRs were performed targeting five virulence genes (stx, lt, st, astA, and eae) and three groups of antibiotic-resistant genes, namely ß-lactamases (extended-spectrum ß-lactamase and AmpC), tetracycline (tetA, tetB, and tetM) and sulfamethoxazole/trimethoprim (sul1, sul2, and sul3). The stx virulence gene, encoding the Shiga/Vero (VT) toxin produced by the verotoxin-producing E. coli (VTEC), was identified with similar frequency in isolates from food (5/31) and water (6/28). The highest percentages of resistant isolates from food and water were found for ß-lactams imipenem (35.5 and 39.3%, respectively) and ampicillin (39.3 and 46.4%, respectively). Multidrug resistance was observed in 31.3% of the isolates, being higher in E. coli isolates from water (45.5%) compared to RTE street food isolates (19.2%). Virulence genes were detected in 73% of the multidrug-resistant isolates. Concerning antibiotic-resistant genes, ESBL was the most frequent (57.7%) among β-lactamases while tetA was the most frequent (50%) among non-β-lactamases.

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In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods

Cited by 8 publications

References 36 publications

Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal

Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal

Multiplex-PCR for diagnosis of Entamoeba histolytica and Escherichia coli O157 in the patients of Wasit Province, Iraq

Characterization of Escherichia coli from Water and Food Sold on the Streets of Maputo: Molecular Typing, Virulence Genes, and Antibiotic Resistance

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