Prevalence and molecular characterization of porcine enteric caliciviruses and first detection of porcine kobuviruses in US swine

Sisay, Zufan; Wang, Qiuhong; Oka, Takahiro; Saif, Linda J.

doi:10.1007/s00705-013-1619-5

Cited by 31 publications

(25 citation statements)

References 32 publications

(45 reference statements)

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“…In addition, similar sapovirus strains were detected from gastroenteritis patients, wastewater, and oysters, which were collected from geographically related areas in the same season (241). In contrast, sapoviruses genetically indistinguishable from those detected in human clinical specimens have not been discovered in other animals (i.e., swine, mink, bats, dogs, and sea lions) (48,88,123,172,173,(249)(250)(251)(252)(253). Based on complete VP1 sequences, GV.3 porcine sapoviruses are closest to human strains; however, they can be clearly separated into a different genotype (Fig.…”

Section: Sapoviruses In Seafood Environmental Water and Animalsmentioning

confidence: 88%

Comprehensive Review of Human Sapoviruses

et al. 2015

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SUMMARY Sapoviruses cause acute gastroenteritis in humans and animals. They belong to the genus Sapovirus within the family Caliciviridae . They infect and cause disease in humans of all ages, in both sporadic cases and outbreaks. The clinical symptoms of sapovirus gastroenteritis are indistinguishable from those caused by noroviruses, so laboratory diagnosis is essential to identify the pathogen. Sapoviruses are highly diverse genetically and antigenically. Currently, reverse transcription-PCR (RT-PCR) assays are widely used for sapovirus detection from clinical specimens due to their high sensitivity and broad reactivity as well as the lack of sensitive assays for antigen detection or cell culture systems for the detection of infectious viruses. Sapoviruses were first discovered in 1976 by electron microscopy in diarrheic samples of humans. To date, sapoviruses have also been detected from several animals: pigs, mink, dogs, sea lions, and bats. In this review, we focus on genomic and antigenic features, molecular typing/classification, detection methods, and clinical and epidemiological profiles of human sapoviruses.

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Section: Sapoviruses In Seafood Environmental Water and Animalsmentioning

confidence: 88%

Comprehensive Review of Human Sapoviruses

et al. 2015

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“…The species Aichivirus C consists of a single type, PKV-1. The existence of PKV-1 in pigs has been well documented in different countries worldwide (Amimo et al, 2014;Barry et al, 2011;Chen et al, 2013;Di Bartolo et al, 2015;Fan et al, 2013;Khamrin et al, 2009Khamrin et al, , 2010Okitsu et al, 2012;Park et al, 2010;Reuter et al, 2013;Sisay et al, 2013); however, in Vietnam, PKV-1 has only been reported from river water (Inaba et al, 2014).…”

Section: Porcine Kobuvirus (Pkv) Was First Identified In 2008 Inmentioning

confidence: 99%

Large-scale screening and characterization of enteroviruses and kobuviruses infecting pigs in Vietnam

Dũng

Anh

Cương

et al. 2016

Journal of General Virology

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A recent survey of pigs in Dong Thap province, Vietnam identified a high frequency of enterovirus species G (EV-G) infection (144/198; 72.7 %). Amongst these was a plethora of EV-G types (EV-G1, EV-G6 and four new types EV-G8-EV-G11). To better characterize the genetic diversity of EV-G and investigate the possible existence of further circulating types, we performed a larger-scale study on 484 pig and 45 farm-bred boar faecal samples collected in 2012 and 2014, respectively. All samples from the previous and current studies were also screened for kobuviruses. The overall EV infection frequency remained extremely high (395/484; 81.6 %), but with comparable detection rates and viral loads between healthy and diarrhoeic pigs; this contrasted with less frequent detection of EV-G in boars (4/45; 8.9 %). EV was most frequently detected in pigs j14 weeks old (,95 %) and declined in older pigs. Infections with EV-G1 and EV-G6 were most frequent, whilst less commonly detected types included EV-G3, EV-G4 and EV-G8-EV-G11, and five new types (EV-G12-EV-G16). In contrast, kobuvirus infection frequency was significantly higher in diarrhoeic pigs (40.9 versus 27.6 %; P50.01). Kobuviruses also showed contrasting epizootiologies and age associations; a higher prevalence was found in boars (42 %) compared with domestic pigs (29 %), with the highest infection frequency amongst pigs .52 weeks old. Although genetically diverse, all kobuviruses identified belonged to the species Aichivirus C. In summary, this study confirms infection with EV-G was endemic in Vietnamese domestic pigs and exhibits high genetic diversity and extensive inter-type recombination.

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“…PKV is a non-enveloped, singlestranded, positive-sense RNA virus that was fi rst detected in Hungary in early 2007 (Reuter et al, 2008), and has now been detected worldwide (Khamrin et al, 2010;Sisay et al, 2013;Yu et al, 2009). While the pathogenesis of PKV is relatively unknown, Park et al suggested that PKV could be the etiological agent of gastroenteritis in pigs based on statistical analysis (Park et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus

Zp¹,

Yang²,

Wd³

et al. 2016

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Summary. -Piglet diarrhea epidemics result in major economic losses for the swine industry. Four viruses are closely linked to porcine diarrhea: porcine kobuvirus (PKV), porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), and porcine rotavirus (PRoV). We have conducted an epidemiology study to determine the frequency of infection and co-infection with these viruses in China, and characterized the genetic variation of the isolated PEDV and PKV strains. Stool and intestinal samples (n = 314) were collected from piglets with diarrhea in China from years 2012 to 2014. RT-PCR was used to detect PKV, PEDV, TGEV, and PRoV. Phylogenetic relationships between reference strains and the isolated PEDV and PKV strains were determined based on the M and 3D gene sequence. Th e rates of infection with PKV, PEDV, TGEV and PRoV were 29.9%, 24.2%, 1.91%, and 0.31%, respectively. Co-infections with PKV and the other three viruses were very common. Co-infection of PKV and PEDV was detected in 15.0% (47/314) of the samples. Phylogenetic analysis of the PKV 3D gene indicated that there were some phylogenetic diff erences in the PKV strains across regions within China. However, according to the PEDV M gene, strains clustered into three groups and the primary group was distinct from the vaccine strain CV777. Th is study provides insights in to the prevalence of diarrhea viruses and their prevention and control in China.Keywords: porcine diarrhea viruses; co-infection; porcine kobuvirus; porcine epidemic diarrhea virus; porcine gastroenteritis; porcine rotavirus * Corresponding authors: E-mail: wenjiejin1@163.com, aijian@ yzu.edu.cn; phone: +8613815809571.# Th ese authors contributed equally to this work. Abbreviations: PKV = porcine kobuvirus; PEDV = porcine epidemic diarrhea virus; TGEV = porcine transmissible gastroenteritis virus; ProV = porcine rotavirus

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Prevalence and molecular characterization of porcine enteric caliciviruses and first detection of porcine kobuviruses in US swine

Cited by 31 publications

References 32 publications

Comprehensive Review of Human Sapoviruses

Comprehensive Review of Human Sapoviruses

Large-scale screening and characterization of enteroviruses and kobuviruses infecting pigs in Vietnam

The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus

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