Prevalence and genotypes of Toxoplasma gondii in pork from retail meat stores in Eastern China

Wang, Hua; Wang, Teng; Luo, Qingli; Huo, Xingxing; Wang, Lin; Liu, Tingting; Xu, Xiaojun; Wang, Yong; Lü, Fangli; Lun, Zhao‐Rong; Yu, Li; Shen, Ji‐Long

doi:10.1016/j.ijfoodmicro.2012.06.011

Cited by 56 publications

(30 citation statements)

References 40 publications

(46 reference statements)

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“…In this study, ToxoDB#9 was identified to be prevalent in this region. In previous studies, this same genotype was frequently identified in cats in many regions in China (Dubey et al 2007;Chen et al 2011;Qian et al 2012;Wang et al 2013;Tian et al 2014), and it was also found in humans, pigs, goats, and other species Wang et al 2012;Cong et al 2015;Miao et al 2015). Hence, ToxoDB#9 is considered to be a preponderant lineage prevalent in China.…”

Section: Discussionmentioning

confidence: 69%

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

et al. 2015

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This study was conducted to estimate the prevalence of Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections among stray and pet cats in Lanzhou, northwest China, and to identify the influence of age, gender, and regions on seropositivity. T. gondii antibodies were examined in cat sera by the modified agglutination test (MAT). The circulating antigens of D. immitis and FeLV and specific antibodies to FIV were examined using kits commercially available. The overall prevalence of T. gondii, FIV, FeLV, and D. immitis was 19.34, 9.12, 11.33, and 3.04 %, respectively. For the genetic characterization of T. gondii genotypes in cats, genomic DNA was extracted from the seropositive cats and the T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genotyped using multilocus PCR-RFLP. Two T. gondii genotypes (ToxoDB#9 and ToxoDB#1) were identified. Results of the multivariate logistic regression analysis showed that older cats are more likely to be seropositive than juveniles for T. gondii, FIV, FeLV, and D. immitis. This is the first report of T. gondii genotypes in cats in northwest China. Moreover, the present study is the first study of retrovirus and D. immitis seroprevalence in cats in China. The results revealed that T. gondii, FIV, and FeLV infections are common in stray and pet cats in northwest China.

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Section: Discussionmentioning

confidence: 69%

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

et al. 2015

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“…Also, once T. gondii cysts have formed in the brain and muscles, toxoplasmosis does not usually show any clinical signs or visible damage in immune competent animals, a situation that may lead to underestimates of the disease prevalence. Indeed, a study from Anhui province, eastern China, found that a significant proportion (18.03%) of commercially available pork is contaminated with T. gondii based on the real-time PCR results [14]. …”

Section: Discussionmentioning

confidence: 99%

“…Despite the large literary data on toxoplasmosis worldwide, studies on the occurrence and genetic characterization of T. gondii isolates from pigs in China are rather scarce [10–16], although pork is one of the most popular meats, representing around 65% of meat consumption in this country [14]. Henan, central China, is one of the biggest producers of pork products in China.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Swine Toxoplasmosis by PCR and Genotyping of Toxoplasma gondii from pigs in Henan, Central China

et al. 2017

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Background Toxoplasma gondii, a widely prevalent protozoan parasite, causes serious toxoplasmosis infections in humans and other animals. Among livestock, pigs are susceptible to T. gondii infection. Despite Henan being one of the biggest pig-raising provinces in China, little information exists on the epidemiology of toxoplasmosis in this location. Therefore, we molecularly characterized DNA samples from pigs in Henan. A total of 1647 samples, including 952 from dead piglets, 478 from seriously sick fattening pigs and 217 from abortion sows, were collected from different animal hospitals or pig farms from 10 different cities in Henan (2006–2008). Each pig corresponded to a separate pig farm. DNA was extracted from 3 to 5 g of the most severely affected pig tissue (liver, spleen, lung, hilar lymph nodes and amniotic fluid) after postmortem examination. The presence of the T. gondii B1 gene was detected using nested polymerase chain reactions (PCR). Genotyping was performed directly on DNA from the PCR-positive tissue samples using 11 PCR restriction fragment length polymorphism markers (SAG1, 5′- and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22–8, c29–2, and Apico).ResultsOf all samples, thirty-four were positive for the T. gondii B1 gene (2.06%, 95% CI: 1.86%–2.26%) from four cities, including 31 from NanYang city, one (PgXY 1) from Xinyang City, one (PgZZ 1) from Zhengzhou City and one (PgZK1) from Zhoukou City. The prevalence was found to be highest in piglets than in fattening pigs and sows. And the difference was statistically significant (P<0.01). The following 32 samples were genotyped with complete data: 13 hilar lymph node tissue samples, seven liver tissue samples, seven lung tissue samples, four spleen tissue samples, and one amniotic fluid sample. Only one genotype, belonging to ToxoDB Genotype #9, was identified.ConclusionsThis is the first large-scale survey molecularly characterizing T. gondii from pigs in Henan. The results of the present study revealed that T. gondii infection is present in swine in Henan and is a potential source of foodborne toxoplasmosis in the investigated areas. Implementation of effective control measures for T. gondii to reduce the chance of zoonotic toxoplasmosis spreading from pig farms may be warranted. The results show that the ToxoDB #9 genotype may be the dominant T. gondii lineage in mainland China. These findings strengthen the limited Chinese T. gondii epidemiology database.

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“…There are no details concerning the other two human isolates. Thirteen isolates from pigs in China have been genotyped and reported in literature Wang et al, 2012aWang et al, ,b,c, 2013aLi et al, 2015), revealing genotype #9 for 11 strains and #10 for two strains. In addition, genotype #3 (1 strain) was reported in a sheep ) and #225 (1 strain) in a chicken (Wang et al, 2013a).…”

Section: Discussionmentioning

confidence: 99%

Isolation and genetic characterization of viable Toxoplasma gondii from tissues and feces of cats from the central region of China

Yang

Ying

Verma

et al. 2015

Veterinary Parasitology

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Prevalence and genotypes of Toxoplasma gondii in pork from retail meat stores in Eastern China

Cited by 56 publications

References 40 publications

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

Diagnosis of Swine Toxoplasmosis by PCR and Genotyping of Toxoplasma gondii from pigs in Henan, Central China

Isolation and genetic characterization of viable Toxoplasma gondii from tissues and feces of cats from the central region of China

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