Prevalence and genome characterization of porcine rotavirus A in southern Mozambique

Boene, Simone; João, Eva D.; Strydom, Amy; Munlela, Benilde; Chissaque, Assucênio; Bauhofer, Adilson Fernando Loforte; Nabetse, Elvino; Latifo, Dalilo; Cala, Aida; Mapaco, Lourenço; Chilaúle, Jorfélia; O’Neill, Hester G.; Deus, Nilsa de

doi:10.1016/j.meegid.2020.104637

Cited by 11 publications

(15 citation statements)

References 33 publications

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“…Recently, the G3P[9] rotavirus strain was detected in a two-year-old female cat with severe diarrhea in Bangkok in 2021 [ 54 ]. Overall, the prevalence and predominant strains of rotavirus A in domestic animals in Thailand align with the findings from other studies [ 55 , 56 , 57 ].…”

Section: Molecular Epidemiology and Genetic Diversitysupporting

confidence: 88%

Genetic Diversity of Rotaviruses Circulating in Pediatric Patients and Domestic Animals in Thailand

2023

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Rotavirus A is a highly contagious virus that causes acute gastroenteritis in humans and a wide variety of animals. In this review, we summarized the information on rotavirus described in the studies in the last decade (2008 to 2021) in Thailand, including the prevalence, seasonality, genetic diversity, and interspecies transmission. The overall prevalence of rotavirus infection in humans ranged from 15–33%. Rotavirus infection was detected throughout the year and most frequently in the dry and cold months, typically in March. The diversity of rotavirus genotypes varied year to year and from region to region. From 2008 to 2016, rotavirus G1P[8] was detected as the most predominant genotype in Thailand. After 2016, G1P[8] decreased significantly and other genotypes including G3P[8], G8P[8], and G9P[8] were increasingly detected from 2016 to 2020. Several uncommon rotavirus strains such as G1P[6], G4P[6], and G3P[10] have also been occasionally detected. In addition, most studies on rotavirus A infection in animals in Thailand from 2011 to 2021 reported the detection of rotavirus A in piglets and canine species. It was reported that rotavirus could cross the host species barrier between humans and animals through interspecies transmission and genetic reassortment mechanisms. The surveillance of rotavirus infection is crucial to identify the trend of rotavirus infection and the emergence of novel rotavirus genotypes in this country. The data provide information on rotavirus infection and the diversity of rotavirus genotypes circulating in the pre-vaccination period, and the data will be useful for the evaluation of the effectiveness of rotavirus vaccine implementation in Thailand.

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Section: Molecular Epidemiology and Genetic Diversitysupporting

confidence: 88%

Genetic Diversity of Rotaviruses Circulating in Pediatric Patients and Domestic Animals in Thailand

2023

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“…The few animals in the households we sampled may have limited the spread of rotavirus, thus contributing to the low rotavirus prevalence observed. A study in Mozambique also found a low prevalence of rotavirus in the pigs from small holdings [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals

Bwogi

Karamagi

Byarugaba

et al. 2023

Viruses

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Rotavirus genotypes are species specific. However, interspecies transmission is reported to result in the emergence of new genotypes. A cross-sectional study of 242 households with 281 cattle, 418 goats, 438 pigs, and 258 humans in Uganda was undertaken between 2013 and 2014. The study aimed to determine the prevalence and genotypes of rotaviruses across co-habiting host species, as well as potential cross-species transmission. Rotavirus infection in humans and animals was determined using NSP3 targeted RT-PCR and ProSpecT Rotavirus ELISA tests, respectively. Genotyping of rotavirus-positive samples was by G- and P-genotype specific primers in nested RT-PCR assays while genotyping of VP4 and VP7 proteins for the non-typeable human positive sample was done by Sanger sequencing. Mixed effect logistic regression was used to determine the factors associated with rotavirus infection in animals. The prevalence of rotavirus was 4.1% (95% CI: 3.0–5.5%) among the domestic animals and 0.8% (95% CI: 0.4–1.5%) in humans. The genotypes in human samples were G9P[8] and P[4]. In animals, six G-genotypes, G3(2.5%), G8(10%), G9(10%), G11(26.8%), G10(35%), and G12(42.5%), and nine P-genotypes, P[1](2.4%), P[4](4.9%), P[5](7.3%), P[6](14.6%), P[7](7.3%), P[8](9.8%), P[9](9.8%), P[10](12.2%), and P[11](17.1%), were identified. Animals aged 2 to 18 months were less likely to have rotavirus infection in comparison with animals below 2 months of age. No inter-host species transmission was identified.

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“…Globally, we face a prevalence of emerging RVA strains with seemingly increased interspecies transmission potential, such as G9P[13] [ 51 , 52 , 53 , 54 , 55 ]. Numerous studies evaluating RVA pathogenesis, immune response, epidemiology, and interventions often yield inconsistent results because they are not designed to account for the drastic differences of the host response to genetically diverse RVAs.…”

Section: Discussionmentioning

confidence: 99%

Host Cell Response to Rotavirus Infection with Emphasis on Virus–Glycan Interactions, Cholesterol Metabolism, and Innate Immunity

Raque

Raev

Guo

et al. 2023

Viruses

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Although rotavirus A (RVA) is the primary cause of acute viral gastroenteritis in children and young animals, mechanisms of its replication and pathogenesis remain poorly understood. We previously demonstrated that the neuraminidase-mediated removal of terminal sialic acids (SAs) significantly enhanced RVA-G9P[13] replication, while inhibiting RVA-G5P[7] replication. In this study, we compared the transcriptome responses of porcine ileal enteroids (PIEs) to G5P[7] vs. G9P[13] infections, with emphasis on the genes associated with immune response, cholesterol metabolism, and host cell attachment. The analysis demonstrated that G9P[13] infection led to a robust modulation of gene expression (4093 significantly modulated genes vs. 488 genes modulated by G5P[7]) and a significant modulation of glycosyltransferase-encoding genes. The two strains differentially affected signaling pathways related to immune response, with G9P[13] mostly upregulating and G5P[7] inhibiting them. Both RVAs modulated the expression of genes encoding for cholesterol transporters. G9P[13], but not G5P[7], significantly affected the ceramide synthesis pathway known to affect both cholesterol and glycan metabolism. Thus, our results highlight the unique mechanisms regulating cellular response to infection caused by emerging/re-emerging and historical RVA strains relevant to RVA-receptor interactions, metabolic pathways, and immune signaling pathways that are critical in the design of effective control strategies.

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Prevalence and genome characterization of porcine rotavirus A in southern Mozambique

Cited by 11 publications

References 33 publications

Genetic Diversity of Rotaviruses Circulating in Pediatric Patients and Domestic Animals in Thailand

Genetic Diversity of Rotaviruses Circulating in Pediatric Patients and Domestic Animals in Thailand

Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals

Host Cell Response to Rotavirus Infection with Emphasis on Virus–Glycan Interactions, Cholesterol Metabolism, and Innate Immunity

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