Prevalence and Diversity of Salmonella Serotypes in Ecuadorian Broilers at Slaughter Age

Vinueza-Burgos, Christian; Cevallos, María; Ron, Lenin; Bertrand, Sophie; Zutter, Lieven De

doi:10.1371/journal.pone.0159567

Cited by 66 publications

(63 citation statements)

References 53 publications

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“…The prevalence of S. Infantis in poultry was found 22Á6% which is in accordance with the previous report from the Republic of Srpska where the authors found 26Á8% prevalence of S. Infantis followed by S. Enteritidis (5Á5%) among 108 Salmonella isolates (Kalaba et al 2017). But this prevalence is lower than the 83Á9% found in another study from Ecuador followed by S. Enteritidis (14Á5%) and S. Corvallis (1Á6%) (Vinueza-Burgos et al 2016). The differences between reports may be linked to various factors such as geographic location, sample selection criteria, the season of the study and hygienic environments of different farms.…”

Section: Discussionsupporting

confidence: 89%

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Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

et al. 2019

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Aims The aim of this study was the molecular identification of Salmonella enterica serovar Infantis (S. Infantis) isolated from poultry samples and their antimicrobial resistance and virulence profiling. Methods and Results A total of 149 isolates, belonging to genus Salmonella, originally isolated from 340 suspected poultry post mortem specimens reported by us earlier were preliminary identified as Salmonella by biochemical methods and confirmed by polymerase chain reaction targeting genus‐specific gene invA. Targeting serovar‐specific gene fragment (fljB) resulted in confirmation of 54 isolates as S. Infantis which were further confirmed by sequencing of 16S RNA and fljB genes. Swimming and swarming motilities were detected in 98·1 and 11·1% isolates respectively. Phenotypic disc diffusion assay against 23 antimicrobial agents showed the highest resistance against pefloxacin (PEF) (94·4%), chloramphenicol (83·3%) and imipenem (77·7%) while 5·3% isolates showed extended‐spectrum β‐lactamase production. Fifty‐nine genes reported for antimicrobial resistance and 12 for conferring virulence were targeted. The most prevalent resistance gene for aminoglycosides was aadA (42·3%), for quinolone resistance determining region parE (62·5%), for penicillin's Int1 (62·9%), for chloramphenicol cat3 (66·1%) and for beta‐lactams blaTEM‐1 (44·4%). Among efflux pump coding genes, armA showed highest (74·2%) prevalence and for virulence, a high prevalence of SopE (89·2%) showed the zoonotic potential of the isolates. The activity of efflux pumps was detected through Ethidium Bromide‐agar method. Conclusions Poultry could act as reservoirs of multidrug resistance Salmonella. Significance and Impact of the Study We firstly report the prevalence and molecular characterization of virulence/drug resistance in S. Infantis from this region and the results may contribute to designing precisely targeted therapy. This study has also highlighted the possible emergence of S. Infantis with zoonotic potential.

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Section: Discussionsupporting

confidence: 89%

“…Corvallis (1·6%) (Vinueza‐Burgos et al . ). The differences between reports may be linked to various factors such as geographic location, sample selection criteria, the season of the study and hygienic environments of different farms.…”

Section: Discussionmentioning

confidence: 97%

Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

et al. 2019

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“…enterica depend on warm-blooded animals and humans for habitat (Brenner, Villar, Angulo, Tauxe, & Waminathan, 2000;Grimont & Weill, 2007). Although almost all species of domesticated animals harbour Salmonella, poultry under intensive production systems are represented more frequently (Akbarmehr, 2011;Paião et al, 2013;Vinueza-Burgos, Cevallos, Ron-Garrido, Bertrand, & Zutter, 2016).…”

Section: Salmon Ell a Infec Ti On In Dome S Tic Animal Smentioning

confidence: 99%

Infection, colonization and shedding of Campylobacter and Salmonella in animals and their contribution to human disease: A review

Rukambile

Sintchenko

Muscatello

et al. 2019

Zoonoses and Public Health

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Livestock meat and offal contribute significantly to human nutrition as sources of high‐quality protein and micronutrients. Livestock products are increasingly in demand, particularly in low‐ and middle‐income settings where economies are growing and meat is increasingly seen as an affordable and desirable food item. Demand is also driving intensification of livestock keeping and processing. An unintended consequence of intensification is increased exposure to zoonotic agents, and a contemporary emerging problem is infection with Campylobacter and Salmonella spp. from livestock (avian and mammalian), which can lead to disease, malabsorption and undernutrition through acute and chronic diarrhoea. This can occur at the farm, in households or through the food chain. Direct infection occurs when handling livestock and through bacteria shed into the environment, on food preparation surfaces or around the house and surroundings. This manuscript critically reviews Campylobacter and Salmonella infections in animals, examines the factors affecting colonization and faecal shedding of bacteria of these two genera as well as risk factors for human acquisition of the infection from infected animals or environment and analyses priority areas for preventive actions with a focus on resource‐poor settings.

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“…suggests that cross contamination between farms is a plausible hypothesis. This idea is 322 supported by other studies that found that Salmonella and Campylobacter isolated from 323 different poultry farms are clonally related [67]. In Ecuador, climatic and social factors 324 lead to most poultry houses having an open configuration in which implementation of 325 rigorous biosecurity is difficult.…”

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Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador

Vinueza-Burgos

Ortega-Paredes

Narváez

et al. 2018

Preprint

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Antimicrobial resistance (AR) is a worldwide concern. Up to a 160% increase in antibiotic usage in food animals is expected in Latin American countries. The poultry industry is an increasingly important segment of food production and contributor to AR. The objective of this study was to evaluate the prevalence, AR patterns and the characterization of relevant resistance genes in Extended Spectrum β-lactamases (ESBL) and AmpC E. coli from large poultry farms in Ecuador. Sampling was performed from June 2013 to July 2014 in 6 slaughterhouses that slaughter broilers from 115 farms totaling 384 flocks. Each sample of collected caeca was streaked onto TBX agar supplemented with cefotaxime (3 mg/l). In total, 176 isolates were analyzed for antimicrobial resistance patterns by the disk diffusion method and for blaCTX-M, blaTEM, blaCMY, blaSHV, blaKPC, and mcr-1 by PCR and sequencing. ESBL and AmpC E. coli were found in 362 flocks (94.3%) from 112 farms (97.4%). We found that 98.3% of the isolates were multi-resistant to antibiotics. Low resistance was observed for ertapenem and nitrofurantoin. The most prevalent ESBL genes were the blaCTX-M (90.9%) blaCTX-M-65, blaCTX-M-55 and blaCTX-M-3 alleles. Most of the AmpC strains presented the blaCMY-2 gene. Three isolates showed the mcr-1 gene. Poultry production systems represent a hotspot for antimicrobial resistance in Ecuador, possibly mediated by the extensive use of antibiotics. Monitoring this sector in national and regional plans of antimicrobial resistance surveillance should therefore be considered.

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Prevalence and Diversity of Salmonella Serotypes in Ecuadorian Broilers at Slaughter Age

Cited by 66 publications

References 53 publications

Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

Detection and characterization of multidrug‐resistant Salmonella enterica serovar Infantis as an emerging threat in poultry farms of Faisalabad, Pakistan

Infection, colonization and shedding of Campylobacter and Salmonella in animals and their contribution to human disease: A review

Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador

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