Prevalence and distribution of six bee viruses in Korean Apis cerana populations

Choe, Se Eun; Nguyen, Lien T.; Noh, Jin Hyeong; Koh, Hong Bum; Jean, Young-Hwa; Kweon, Chang-Hee; Kang, Seung Won

doi:10.1016/j.jip.2012.01.003

Cited by 47 publications

(38 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results reported by Bailey et al [4], Tentcheva et al [12], Gauthier et al [6] and Runckel et al [51] show that ABPV is known to persist in apparently healthy colonies. The proportion of 61.8% samples positive to ABPV found in this study is in accordance with the estimates obtained in France (58%) [12], Austria (68%) [13], Hungary (37%) [15], Germany (73%) [33] and Slovenia (40%) [47], but disagrees with fi ndings of researchers in Denmark (10.56%) [38], China (6%) [18], South Korea (0%) [52] and Uruguay (9%) [50]. High prevalence of ABPV in Serbian samples could also be explained by the mite-virus relationship, sampling period and apitechnical measures mentioned earlier.…”

Section: Discussionsupporting

confidence: 86%

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Simeunović¹,

Stevanović²,

Vidanović³

et al. 2014

Acta Veterinaria

View full text Add to dashboard Cite

In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.

show abstract

Section: Discussionsupporting

confidence: 86%

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Simeunović¹,

Stevanović²,

Vidanović³

et al. 2014

Acta Veterinaria

View full text Add to dashboard Cite

show abstract

“…This phenomenon has also been reported in many countries including France, Hungary, USA, Austria, Southwest England and South Korea [7]. The dynamics of these coexisting virus populations within these colonies is of great interest and it would be useful to determine how they fluctuate with respect to each other [28]. Therefore, the incidence of dual and triple infections confirmed in this study is unsurprising.…”

Section: Discussionsupporting

confidence: 69%

Prevalence of Four Common Bee RNA Viruses in Eastern Bee Populations in Yunnan Province, China

Wang¹,

Bi²

2015

J Veterinar Sci Technol

View full text Add to dashboard Cite

Deformed wing virus (DWV), black queen cell virus (BQCV), sacbrood virus (SBV), Kashmir bee virus (KBV), acute bee paralysis virus (ABPV) and chronic bee paralysis virus (CBPV) are the most common RNA viruses in bee population worldwide. To determine the prevalence of these viruses in Apis cerana in Yunnan Province, China, 270 adult Eastern worker bee (A. cerana) samples have been collected from nine major bee breeding regions in Yunnan province for screening. Our data showed that BQCV, DWV, KBV and SBV are widely prevalent in Yunnan Province. The total prevalence rates of KBV, DWV, SBV and BQCV in Yunnan province were 3%, 14.8%, 24.1% and 36.3%, respectively. BQCV was detected in all selected regions except Jinghong City. The prevalence of KBV in Tengchong County is the first evidence, to our knowledge, showing that Eastern bees could be infected by KBV. The highest infection rate of SBV was found in Kunming city (56.7%). Sequence analysis demonstrated high nucleotide homology between the isolated BQCV, DWV, KBV and SBV strains. Phylogenetic analysis showed that except SBV (YN07), Yunnan isolates can be likely clustered into independent branches, which indicates the possibility of geographic origins.

show abstract

“…With this caveat in mind, in this study, the frequency of infection by Nosema was slightly lower in larvae than in adults, A. apis infection was more prevalent in larvae and pupae than in adults and infection by P. larvae was the highest in the pupae. Elsewhere, as already mentioned earlier, SBV infection frequencies in A. cerana in China was higher in adults (86%) and then larvae (79%) than in pupae (33%) (Ai et al, 2012), whilst in South Korea the SBV infection frequency in A. cerana was also higher in larvae (60.5%) than in adults (30.7%) (Choe et al, 2012). Furthermore, pupae of A. cerana were highly susceptible to infection by P. larvae vegetative cells, whilst 1-d-old larvae were the most susceptible to infection by P. larvae spores (Chen et al, 2000).…”

Section: Discussionsupporting

confidence: 68%

“…For example, the low prevalence (0.90%) of detected SBV infections in A. mellifera in Germany (Hedtke et al, 2011), and the very high SBV infection frequencies in A. cerana in China (Ai et al, 2012), at 86% for adults, 33% for pupae and 79% for larvae, and in South Korea at 60.5% in larvae and 30.7% in adults (Choe et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Pathogen detection and gut bacteria identification in Apis cerana indica in Thailand

Nonthapa¹,

Chanchao²

2015

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Pathogen infection of honeybees can lead to economic losses in apiculture. The earlier the pathogen contamination can be found the better it will likely be for the treatment of the infected colony and prevention of the spread of the pathogen within and between colonies. A total of 50 colonies of Apis cerana were sampled in Samut-Songkhram (five colonies) and Chumphon (45 colonies) provinces in the central and the south of Thailand, respectively. Diagnostic multiplex polymerase chain reaction (PCR) revealed that 20, 6, 4, 20 and 0% of the samples were infected by Paenibacillus larvae, Nosema ceranae, Nosema apis, Ascosphaera apis and Sacbrood virus (Morator aetatulus), respectively. Positive amplified PCR products of target genes were sequenced. A phylogenetic tree was constructed by the neighbor-joining (NJ) distance based, using the Phylogenetic Analysis Using Parsimony (PAUP 4.0b10) program. The phylogenetic relationship of each pathogen, based on the partial sequence of the 16S rRNA of P. larvae, N. ceranae and N. apis, and of the 5.8S rRNA of A. apis, revealed a significant difference from the non-Thai isolates of these pathogens, but no significant geographical isolation between the different Thai apiaries, although it separated some into closely related clusters. In order to reduce the use of antibiotics in an apiary, bacteria in the gut of healthy bees were focused. Interestingly, Bifidobacterium species, Lactobacillus species, Bacillus species, Lactobacillus spp. and other lactic acid bacteria, were isolated from larvae and adult workers, but gave conflicting preliminary identities based on their biochemistry-morphology versus sequence analysis of a partial fragment (1.4 kb) of their 16S rRNA.

show abstract

Prevalence and distribution of six bee viruses in Korean Apis cerana populations

Cited by 47 publications

References 20 publications

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Prevalence of Four Common Bee RNA Viruses in Eastern Bee Populations in Yunnan Province, China

Pathogen detection and gut bacteria identification in Apis cerana indica in Thailand

Contact Info

Product

Resources

About