A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Simeunović, Predrag; Stevanović, Jevrosima; Vidanović, Dejan; Nišavić, Jakov; Radović, Dejan; Stanišić, Ljubodrag; Stanimirović, Zoran

doi:10.2478/acve-2014-0009

Cited by 16 publications

(18 citation statements)

References 49 publications

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“…In Serbia, the fi rst molecular research into the presence of bee viruses was conducted on 11 apiaries [68] including 55 honey bee colonies from different regions. Real-time RT-PCR detected DWV in all apiaries and ABPV in 10 out of 11.…”

Section: Infestation With Varroa Destructor and Mixed Virus Infectionsmentioning

confidence: 99%

Looking for the causes of and solutions to the issue of honey bee colony losses

et al. 2019

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Colony losses, including those induced by the colony collapse disorder, are an urgent problem of contemporary apiculture which has been capturing the attention of both apiculturists and the research community. CCD is characterized by the absence of adult dead bees in the hive in which few workers and a queen remain, the ratio between the brood quantity and the number of workers is heavily disturbed in favor of the former, and more than enough food is present. Robbing behavior and pests usually attacking the weakened colony do not occur. In the present paper, the causes of the emergence of this problem are discussed, as well as the measures of its prevention. The following factors, which lead to colony losses, are analyzed: shortage of high-quality food (pollen and honey); infestation with parasites, primarily with Varroa destructor, and mixed virus infections; bacterial infections (American and European foulbrood), fungal infections (nosemosis and ascosphaerosis) and trypanosomal infections (lotmariosis); and, finally, general management of the apiary. Certain preventive measures are proposed: (1) providing ample high-quality forage and clean water, (2) avoiding sugarisation, i.e. superfluous use of sugar syrup, (3) meeting the nutritional needs of the colony, (4) when feeding bees, taking care of the timing and the composition of diet, avoiding pure sugar syrup which in excessive quantities may induce energetic and oxidative stress, (5) when there is a shortage of natural feed – honey in the brood chamber – use sugar syrup with natural/artificial supplements to avoid protein starvation, (6) organized control of V. destructor in the colonies is obligatory due to its vector role, and (7) compliance with hygienic and sanitary measures and principles of good apiculture practice and management in apiaries. To conclude, all preventive measures are feasible in compliance with rules and regulations concerning regular spring and autumn bee health monitoring by licensed veterinarians, who can propose adequate treatments if necessary.

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Section: Infestation With Varroa Destructor and Mixed Virus Infectionsmentioning

confidence: 99%

Looking for the causes of and solutions to the issue of honey bee colony losses

et al. 2019

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“…The leading causes of adult bee diseases in honey bees are two microsporidian species of the genus Nosema (kingdom Fungi, class Microsporidia, family Nosematidae) -Nosema apis and N. ceranae along with Varroa destructor mite, honey bee viruses and their interactions [1][2][3]. These parasites cause microsporidiosis (nosemosis), an infection that spreads per os with contaminated food.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effectiveness of Selected Treatments of Nosema Spp. Infection by the Hemocytometric Method and Duplex Pcr

Michalczyk¹,

Sokół²,

Koziatek³

2016

Acta Veterinaria

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Recent years have witnessed an increase in the mortality of honey bees in many regions of the world. The observed decrease in the bee population results from a combination of factors, and microsporidian parasites Nosema apis and N. ceranae are among the main contributors. Those parasites cause a microsporidian infection that shortens the lifespan of bees and reduces the productivity of bee colonies. The aim of this study was to evaluate the effectiveness of Nozevit, Api Herb and ApiX (acetylsalicylic acid + Artemisia absinthium L. extract) in the control of infections caused by Nosema spp. in a fi eld experiment. Two groups of worker bees were evaluated -hive bees and forager bees returning to the hive. The effect of the analyzed therapies on the number of spores and the microsporidia species were analyzed by the hemocytometric method and duplex PCR. A statistical analysis revealed that the applied treatments had reduced the number of spores by 31.15% on average. In hive bees, Nosema spp. infection was most effectively reduced by Nozevit (67.85%) and ApiX (63.36%). Coinfections (N. ceranae and N. apis) were affi rmed in all bee samples before treatments. However, after the treatments, single infection of N. apis and N. ceranae were detected. The tested treatments were more effective in the control of N. apis than N. ceranae.

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“…Studies on bee pathogens causing colony decline in Serbia were mainly focused on Nosema sp. (Stanimirović et al, 2007b;Stevanovic et al, 2011, Stevanović et al, 2013Glavinić et al, 2014;Simeunovic et al, 2014b) and V. destructor (Stanimirović et al, 2002, Stanimirović, Stevanović & Ćirković, 2005Stanimirović et al, 2005;Stevanovic et al, 2008;Stanimirović et al, 2011;Radakovic et al, 2013;Gajic et al, 2013;Glavinić et al, 2014;Stanimirović et al, 2017) with only one study dealing with bee viruses (Simeunović et al, 2014a). Therefore, our work represents an important contribution towards better understanding of bee pathogens in Serbia.…”

Section: Discussionmentioning

confidence: 84%

“…More than 22 honey bee viruses have been identified and described so far (Genersch, 2010;), which exist or co-exist in individual bees or colonies, but may remain unnoticed (Chen & Siede, 2007;Brutscher, McMenamin & Flenniken, 2016). However, several viruses transferred by V. destructor considered to pose increasing risk to colonies' health (Martin et al, 2012) including deformed wing virus (DWV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV) and the sacbrood virus (SBV), all of them seeming to have worldwide occurrence and distribution (Genersch, 2010;Simeunović et al, 2014a;Brutscher, McMenamin & Flenniken, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…In Southeastern Europe, the presence and prevalence of bee viruses have been investigated in Hungary, Slovenia and Croatia (Bakonyi et al, 2002;Forgách et al, 2008;Toplak et al, 2012; Tlak Tlak Gajger, Bičak & Belužić, 2014). In Serbia, four honey bee viruses were reported: ABPV, Egypt bee virus J strain (EBV), cloudy wing virus (CWV) and the black queen cell virus (BQCV) by Kulinčević, Ball & Mladjan (1990), and DWV and ABPV by Simeunović et al (2014a). However, due to the lack of information on the prevalence of SBV and CBPV, as well as the long time which passed since the previous investigations necessity demands newer research.…”

Section: Introductionmentioning

confidence: 99%

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Peer Review #3 of "Honey bee viruses in Serbian colonies of different strength (v0.1)"

2018

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Protection of honey bees is of great economic importance because of their role in pollination. Crucial steps towards this goal are epidemiological surveys of pathogens connected with honey bee losses. In this study deformed wing virus (DWV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV) and sacbrood virus (SBV) were investigated in colonies of different strength located in five regions of Serbia. The relationship between colony strength and virus occurrence/infection intensity were assessed as well as the genetic relationship between virus sequences from Serbia and worldwide. Real-time RT-PCR analyses detected at least one virus in 87.33% of colonies. Single infection was found in 28.67% colonies (21.33%, 4.00%, 2.67% and 0.67% in cases of DWV, ABPV, SBV and CBPV, respectively). In the majority of colonies (58.66%) more than one virus was found. The most prevalent was DWV (74%), followed by ABPV, SBV and CBPV (49.30%, 24.00% and 6.70%, respectively). Except for DWV, the prevalence of the remaining three viruses significantly varied between the regions. No significant differences were found between colony strength and either (i) the prevalence of DWV, ABPV, SBV, CBPV and their combinations, or (ii) DWV infection levels. The sequences of honey bee viruses obtained from bees in Serbia were 93-99% identical with those deposited in GenBank.

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A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Cited by 16 publications

References 49 publications

Looking for the causes of and solutions to the issue of honey bee colony losses

Looking for the causes of and solutions to the issue of honey bee colony losses

Evaluation of the Effectiveness of Selected Treatments of Nosema Spp. Infection by the Hemocytometric Method and Duplex Pcr

Peer Review #3 of "Honey bee viruses in Serbian colonies of different strength (v0.1)"

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