Prevalence and characterization ofSalmonella Gallinarum in the chicken in Korea during 2000 to 2008

Kwon, Yong-Kuk; Kim, A.; Kang, Min-Su; Her, Moon; Jung, Byeong Yeal; Lee, K.-M.; Jeong, Wooseog; An, Byung-Ki; Kwon, Jun-Hun

doi:10.3382/ps.2009-00420

Cited by 50 publications

(32 citation statements)

References 15 publications

(17 reference statements)

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“…The rest 15.91% isolates (n=7) fermented glucose, maltose, rhamnose and dulcitol with or without gas demonstrated provided indication of being S. typhimurium (Table 5). These observations are strongly correlated with the theme of [23,24,27]. A total of 44 (where n=44) isolates were positive for Methyl Red test but negative for VP test indicating characteristics of Salmonella spp.…”

Section: Resultssupporting

confidence: 55%

Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

2016

J App Biol Biotech

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The objectives of this study were to isolate the associated Salmonella spp. from cloacal swabs of broiler and their antibiogram studies. A total of 80 cloacal swabs comprising of 50 samples of apparently healthy broiler and 30 samples of diarrheic broiler were collected from different poultry farms at Sylhet, Bangladesh during Ja nuary to June 2013. The samples were subjected for isolation and identification of Salmonella spp. through a series of conventional bacteriological studies like study of morphology, staining properties, and biochemical characteristics. In results, 48% (n= 24/50) swab samples of healthy broiler and 66.7% (n= 20/30) diarrheic broiler were found to be associated with Salmonella spp. Among the 44 positive Salmonella isolates 47.73% (n=21) were Salmonella pullorum, 36.36% (n=16) isolates were Salmonella gallinarum and the rest 15.91% (n=7) isolates were Salmonella typhimurium. Sensitivity test was conducted against 10 commonly used antibiotics, of which Penicillin-G, Erythromycin, Ampicillin, and Bacitracin were found to be resistant, and Ceftriaxone, Gentamycin, and Choloramphenicol showed considerably better sensitivity as compared to others. It is concluded that Salmonella spp. are present in broiler, and the bacteria can be clinically controlled by using Ceftriaxone, Gentamycin, and Choloramphenicol.

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Section: Resultssupporting

confidence: 55%

Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

2016

J App Biol Biotech

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“…Since most SG strains tested here were isolated in the South and Southeast regions of Brazil, this datum suggests the existence of an endemic clone predominant in these regions. A similar hypothesis was previously proposed by Kwon et al (2010), who applied the PFGE-technique to SG strains isolated in Korea, and could not observe any relationship between year and geographic region of isolation for most SG strains studied.…”

Section: Discussionsupporting

confidence: 52%

ERIC-PCR genotyping of field isolates ofSalmonella entericasubsp.entericaserovar Gallinarum biovars Gallinarum and Pullorum

et al. 2015

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Salmonella Gallinarum (SG) and Salmonella Pullorum (SP) have been classified as biovars belonging to Salmonella enterica subsp. enterica serovar Gallinarum. Genetic diversity among isolates of the same biovar can be detected by DNA fingerprinting techniques which are useful in epidemiological investigations. In this study, we applied the PCR amplification of Enterobacterial Repetitive Intergenic Consensus sequences (ERIC-PCR) to analyse 45 strains of SG and SP, most of which were isolated from diseased poultry of different Brazilian regions over a period of 27 years until 2014. The ERIC-genotypes obtained were used to describe the epidemiological relationship amongst the strains. Our findings showed that there were six ERIC-patterns for SG strains at 80% similarity. In addition, some of the SG isolates recovered from different regions and years clustered with 100% similarity, suggesting that transfer of genotypes between these regions has taken place. The commercial rough vaccine strain 9R showed a unique profile. Meanwhile, more genetic diversity was observed among SP strains where ten ERICpatterns were also formed at 80% similarity.

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“…Although the serovar Gallinarum is an important pathogen in chickens, only limited numbers of subtyping assays, including PFGE and randomly amplified polymorphic DNA, are currently available for investigating field outbreaks. PFGE has been successfully used for subtyping field isolates of the serovar Gallinarum until now (Seo et al, 2006;Kwon et al, 2010). Nevertheless, the assay needs more time and labour compared with the automated MLVA methods used for other Salmonella serovars, and may offer only limited discrimination between possibly unrelated strains in some cases (Kwon et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, PFGE is a gold standard for genotyping the serovar Gallinarum isolates to examine the origin or source of the outbreak. Although PFGE has been used successfully to discriminate the serovar Gallinarum isolates, PFGE may show limited discriminatory power such that the large proportion of isolates may appear identical or differ by few bands (Kwon et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…DNA-based molecular subtyping methods, including pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA, have been commonly used in epidemiological studies of fowl typhoid (Seo et al, 2006;Kwon et al, 2010). Currently, PFGE is a gold standard for genotyping the serovar Gallinarum isolates to examine the origin or source of the outbreak.…”

Section: Introductionmentioning

confidence: 99%

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Multilocus variable-number tandem-repeat analysis for subtypingSalmonella entericaserovar Gallinarum

Kang

Kwon

et al. 2011

Avian Pathology

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Salmonella enterica serovar Gallinarum causes a severe systemic disease, fowl typhoid, primarily in chickens and turkeys, and it remains a disease of worldwide significance. Multilocus variable-number tandem-repeat analysis (MLVA) has proved to be very useful for subtyping other Salmonella serovars. We describe the development of a simple MLVA assay for S. enterica serovar Gallinarum that is comparable with pulsedfield gel electrophoresis (PFGE) in resolution. The genome sequence of S. enterica serovar Gallinarum strain 287/91 was analysed for potential variable-number tandem repeats (VNTRs) and then polymerase chain reaction assays were developed to assess the variability of the loci. Four VNTR markers were selected and used in a multiplex fragment analysis assay. The stability of the VNTR markers was assessed by conducting in vitro passage experiments with two strains (95 clones per strain) over a 30-day period. A MLVA of 68 strains of S. enterica serovar Gallinarum based on the four VNTR loci distinguished 26 allelic profiles. The MLVA assay showed a Simpson's diversity index of 0.918, whereas PFGE analysis produced 23 patterns and had a diversity index of 0.874. Most importantly, the MLVA further discriminated strains having the same PFGE pattern. The MLVA assay is a highly discriminatory genotyping method for S. enterica serovar Gallinarum. Therefore, MLVA can be a useful addition to routine PFGE analysis for molecular epidemiological investigation of fowl typhoid.

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Prevalence and characterization ofSalmonella Gallinarum in the chicken in Korea during 2000 to 2008

Cited by 50 publications

References 15 publications

Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

ERIC-PCR genotyping of field isolates ofSalmonella entericasubsp.entericaserovar Gallinarum biovars Gallinarum and Pullorum

Multilocus variable-number tandem-repeat analysis for subtypingSalmonella entericaserovar Gallinarum

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