Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh

Abstract

The objectives of this study were to isolate the associated Salmonella spp. from cloacal swabs of broiler and their antibiogram studies. A total of 80 cloacal swabs comprising of 50 samples of apparently healthy broiler and 30 samples of diarrheic broiler were collected from different poultry farms at Sylhet, Bangladesh during Ja nuary to June 2013. The samples were subjected for isolation and identification of Salmonella spp. through a series of conventional bacteriological studies like study of morphology, staining properties, and biochemical characteristics. In results, 48% (n= 24/50) swab samples of healthy broiler and 66.7% (n= 20/30) diarrheic broiler were found to be associated with Salmonella spp. Among the 44 positive Salmonella isolates 47.73% (n=21) were Salmonella pullorum, 36.36% (n=16) isolates were Salmonella gallinarum and the rest 15.91% (n=7) isolates were Salmonella typhimurium. Sensitivity test was conducted against 10 commonly used antibiotics, of which Penicillin-G, Erythromycin, Ampicillin, and Bacitracin were found to be resistant, and Ceftriaxone, Gentamycin, and Choloramphenicol showed considerably better sensitivity as compared to others. It is concluded that Salmonella spp. are present in broiler, and the bacteria can be clinically controlled by using Ceftriaxone, Gentamycin, and Choloramphenicol.

Isolation, Characterization and Pathogenicity of the Most Common Bacteria Associated with Gut Health in Egyptian Broiler Chicken Flocks

“…However, Egyptian studies of Sedeik et al (2019) and El-Sheikh et al (2019) showed isolation of Salmonellae from broiler chicks in rates of 7.5% and 16%, respectively. Regarding the colonial morphology and biochemical tests results of Salmonella, parallel results were also described by Akeila et al (2013), Islam et al (2016), Al-Mamun et al (2017 and Mridha et al (2020). Moreover, the serological results of the isolated Salmonellae showed that S. enteritidis and S. kentucky were the most among the isolates.…”

Virulence and antimicrobial resistance profile of non-typhoidal Salmonella enterica serovars recovered from poultry processing environments at wet markets in Dhaka, Bangladesh

“…The studies conducted in Bangladesh, S . Typhimurium was found to be 15.91% in broiler production systems [ 80 ]; 85% in broiler farm samples [ 31 ] and 5% in commercial layer farm settings [ 81 ]. S .…”

Isolation and Serological Identification of Current Salmonella Species Recovered from Broiler Chickens in Egypt

“…Many cultures of Salmonella spp. may also produce large colonies with glossy black centers or may appear as almost completely black (Sujatha et al 2003;Ramya et al 2012;Rabie et al 2012;Islam et al 2016). Our results revealed that a suspected 49 Salmonella isolates out of 500 collected samples were detected by morphological and biochemical examination (Table 1).…”