Prevalence and Characterization of Coagulase Positive Staphylococci from Food Products and Human Specimens in Egypt

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) strains have veterinary and public health importance as they are responsible for a wide range of difficult to treat infections and food poisoning. Two hundred samples (50 samples each of minced meat, beef luncheon, Karish cheese, and human samples (pus swab from open wounds)) were cultured, and MRSA strains were identified using disk diffusion tests and mecA gene-based PCR. A total of 35% (70/200) of the examined samples were confirmed as coagulase-positive S. aureus in minced meat (46%), beef luncheon (44%), Karish cheese (44%), and human samples (22%). The MRSA strains showed resistance to amoxicillin (91.4%), penicillin (97.1%), cefoxitin (85.7%), cephradine (82.9%), tetracycline (57.2%), and erythromycin (52.8%). More than half of the tested S. aureus isolates harbored the mecA gene. The sequence analysis of the mecA gene from the minced meat, Karish cheese, and human samples revealed high genetic similarities between the S. aureus isolates from these sources. In conclusion, our findings indicate a risk for the transmission of the mecA gene of S. aureus across the food chain between humans and animal food products. Further studies should focus on finding additional epidemiological aspects of the MRSA strains in food chain.

Phenotypic characterization of antimicrobial drug resistance of Staphylococcus aureus and S. epidermidis strains isolated from various community infections in Oum El Bouaghi city, Algeria

“…Staphylococcus aureus is one of the most common causes of infections and it's also a normal flora of skin that can enter the body through abrasion, cracks, burn, surgical incisions, cuts and causes pyogenic infections (Sangita et al 2019). This study has revealed that S. aureus is the most predominant micro-organism from pus samples, as earlier studies have indicated (Onemu and Ophori 2013), with the occurrence of 72.7% which is almost similar to the finding in the study conducted in Algeria 64.28 % (Benyagoub et al 2020), but its higher than results obtained in Egypt 38% (Abdeen et al 2021) and in Maroc 19% (Benouda and Elhamzaui 2009). For S. epidermidis the highest occurrence was identified from urine with the rate of 91.4%, probably this is primarily related to the contamination of urine by the commensal flora (Frédéric et al 2008), and according to Longauerova (2006) 2 show the variation of the prevalence of S. epidermidis and S. aureus according to the gender of patients.…”

Occurrence of Multidrug-Resistant Staphylococcus aureus among Humans, Rodents, Chickens, and Household Soils in Karatu, Northern Tanzania

“…Among MDR patterns, CD-E-TE, standing for clindamycin, erythromycin, and tetracycline, was displayed in most of the isolates (34.2 %), and the pattern is identical for humans, chickens, and rodents. Different studies on the resistance profiles of S. aureus have reported similar patterns as well [7,18,32,33,39,40]. Erythromycin and tetracycline are the most commonly used antibiotics in this area, since they are cheap and can be purchased over the counter without a prescription [41].…”

Prevalence and Molecular Characterization of Mycoplasma Species, Pasteurella multocida, and Staphylococcus aureus Isolated from Calves with Respiratory Manifestations

“…A previous study employing the 16S rRNA genes sequencing and microarray analysis revealed six clonal complexes of S. aureus recovered from household cattle and buffaloes in Egypt [54]. Analysis of the mecA gene sequences of S. aureus obtained from different food products and human pus samples reported high nucleotide similarities between the S. aureus strains from different origins [53]. Another genotyping assay using the Staphylococcal protein A (spa) gene sequences of S. aureus strains isolated from cattle and camels in Egypt revealed the presence of eight SPA types; the most common SPA type was t359 that found in five isolates of S. aureus [55].…”

“…Several Egyptian and global studies performed the comparative sequence analysis of S. aureus isolated from bovine subclinical mastitis and various food sources [16,[51][52][53]. A previous study employing the 16S rRNA genes sequencing and microarray analysis revealed six clonal complexes of S. aureus recovered from household cattle and buffaloes in Egypt [54].…”