Prevalence and associated factors for HIV-1 transmitted drug resistance in voluntary clients for counseling and testing in Southern Taiwan

Weng, Ya Wei; Tsai, Hung Chin; Lee, Susan Shin Jung; Wu, Kang; Sy, Cheng Len; Chen, Jui Kuang; Chen, Yao Shen

doi:10.1016/j.jmii.2014.08.002

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…The mutations, M184V and K103N are commonly detected as multi-drug resistance mutations leading to the virological failure of a first-line NRTIs-and NNRTI-based regimens [16,28]. The appearance pattern of these drug resistance mutations was similar with those in previous studies in Indonesia, Malaysia, Taiwan, Turkey, and USA [17,[18][19][20][21][22][23][24][25][26][27][28][29][30][31]. M184V is a common mutation in treated populations; however, it is very rare among…”

Section: Discussionsupporting

confidence: 69%

Genotypic characterization of human immunodeficiency virus type 1 isolated in Bali, Indonesia in 2016

Khairunisa¹,

Masyeni²,

Witaningrum³

et al. 2018

HIV & AIDS Review

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Introduction: Bali is a famous tourism destination in Indonesia, and many international travelers from several foreign countries visit Bali. A large number of human immunodeficiency type 1 (HIV-1) infections was found; however, the genotypes of HIV-1 strains circulating in Bali has not yet been elucidated in detail. In addition, the information on HIV drug resistance (HIVDR) in Bali is limited. Material and methods: HIV-1 pol gene encoding 31 proteases (PR gene) and 37 reverse transcriptase (RT gene) as well as 47 gag and 33 env genes were successfully amplified from DNA samples extracted from peripheral blood mononuclear cells samples of 51 antiretroviral therapy (ART)-experienced and 7 ART-naïve HIV-1-infected individuals residing in Gianyar and Denpasar, Bali. Genotyping analyses were then performed. The assessment of drug resistance mutations was based on the guidelines published by the International Antiviral Society-United States of America (IAS-USA). Results: The major HIV-1 subtype detected in this study was CRF01_AE (94.5%). Recombinant viruses containing CRF01_AE and subtype B genes (5.5%) were also detected. Drug resistance-associated major mutations were detected in 8/31 (25.8%) of RT genes but not in PR genes in integrated proviral DNA derived from ART-experienced individuals. However, drug resistance-associated minor mutations were frequently detected in PR genes derived from most samples. Conclusions: This study revealed that CRF01_AE was the predominant HIV-1 subtype in Bali, Indonesia. In addition, the emergence of drug resistance mutations was evident in proviral DNA derived from ART-experienced individuals, indicating that continuous surveillance is needed in order to monitor the emergence of HIVDR and new viral subtypes in Indonesia.

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Section: Discussionsupporting

confidence: 69%

Genotypic characterization of human immunodeficiency virus type 1 isolated in Bali, Indonesia in 2016

Khairunisa¹,

Masyeni²,

Witaningrum³

et al. 2018

HIV & AIDS Review

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“…However, routine drug resistance testing has not been available to clinicians. According to a previous epidemiological study, the TDR rate is 8.0–11.1% in Taiwan [12–14]. HIV-infected Taiwanese patients receive free HIV care based on the national treatment guidelines.…”

Section: Introductionmentioning

confidence: 99%

Trend of HIV transmitted drug resistance before and after implementation of HAART regimen restriction in the treatment of HIV-1 infected patients in southern Taiwan

et al. 2019

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Background The use of fixed combination antiretroviral therapy with a low genetic barrier for the treatment of patients infected with human immunodeficiency virus (HIV) may affect the local HIV transmitted drug resistance (TDR) pattern. The present study aimed to investigate changes in the prevalence of HIV TDR following the implementation of a fixed regimen of HIV treatment in Taiwan in 2012. Methods TDR was measured in antiretroviral treatment-naïve HIV-1-infected individuals who participated in voluntary counseling and testing between 2007 and 2015 in southern Taiwan. Antiretroviral resistance mutations were interpreted using the HIVdb program from the Stanford University HIV Drug Resistance Database. Results Sequences were obtained from 377 consecutive individuals between 2007 and 2015. The overall prevalence rates of TDR HIV among the study population from 2007 to 2011 and 2012–2015 were 10.6 and 7.9%, respectively. Among the detected mutations, K103 N and V179D + K103R were more frequently observed after 2012. Four HIV-infected patients with K103 N variants were detected after 2012, and 4 of the 5 patients with V179D + K103R variants were found after 2012. No significant differences were observed in the TDRs among nucleoside reverse transcriptase inhibitors (NRTIs), non-NRTIs (NNRTIs), protease inhibitors, multiple drug resistance, and any drug resistance between period 1 (2007–2011) and period 2 (2012–2015). Conclusions A fixed treatment regimen with zidovudine/lamivudine + efavirenz or nevirapine as first-line therapy for treatment-naïve patients infected with HIV did not significantly increase the TDR during the 4-year follow-up period. Due to the increase in NNRTI resistance associated with mutations after 2012, a longer follow-up period and larger sample size are needed in future studies.

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“…Surveillance of TDR in Taiwan has revealed the impact of improved antiretroviral therapy (ART) regimens, although routine pre-treatment genotypic drug resistance testing (GRT) in ART-naïve patients is not conducted in Taiwan due to financial constraints. Previous studies have reported prevalence rates of TDR for protease (PR)/reverse transcriptase (RT) ( pol gene) of 8–11.1% [ 2 , 3 ] in northern Taiwan and 10.6% in southern Taiwan [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

HIV-1 integrase strand-transfer inhibitor resistance in southern Taiwan

Tsai

Chen

et al. 2018

Oncotarget

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The use of antiretroviral therapy has reduced rates of mortality and morbidity in patients with human immunodeficiency virus/acquired immune deficiency syndrome(HIV/AIDS). However, transmission of drug-resistant strains poses a challenge to control the spread of HIV-1. Primary resistance to integrase strand-transfer inhibitors (INSTIs) is rare despite their increased use. The prevalence of transmitted drug resistance (TDR) to INSTIs was 0.9% in northern Taiwan. This study was to analyse the prevalence and risk factors of TDR to INSTIs in southern Taiwan. In this study, we enrolled antiretroviral treatment-naïve HIV-1-infected subjects who underwent voluntary counselling and testing from 2013 to 2016 in southern Taiwan. Genotypic drug resistance, coreceptor tropism (CRT) and INSTI resistance were determined. Logistic regression was used to analyse the risk factors for INSTI polymorphic substitution. Sequences were obtained from 184 consecutive individuals, of whom 96.7% were men who have sex with men and 3.3% were heterosexual. Of the patients, 10% (19/183) had hepatitis B and 33.3% (61/183) had syphilis infection. Subtype B HIV-1 strains were found in 96.1% of the patients. Fifteen patients (8.4%, 15/178) harboured nucleoside reverse transcriptase inhibitors, non-nucleoside reverse transcriptase inhibitors or protease inhibitors resistance. CCR-5 coreceptors were used by 71.4% (130/184) of the patients. None of the patients had INSTI resistance-associated mutations, however 16 patients had INSTI polymorphic substitutions, and they were associated with a higher HIV viral load (p = 0.03, OR 2.4, CI 1.1–5.3) and syphilis infection (p = 0.03, OR 3.7, CI 1.1–12.0). In conclusion, no signature INSTI resistance-associated mutations were detected in our cohort. Continued monitoring of TDR to INSTI is needed due to the increased use of INSTIs.

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Prevalence and associated factors for HIV-1 transmitted drug resistance in voluntary clients for counseling and testing in Southern Taiwan

Abstract: In Southern Taiwan, the HIV TDR rate was 10.6% among those receiving VCT. Our findings suggest that sexual behavior may play an important role in HIV drug-resistant strain transmission.

Cited by 12 publications

References 27 publications

Genotypic characterization of human immunodeficiency virus type 1 isolated in Bali, Indonesia in 2016

Genotypic characterization of human immunodeficiency virus type 1 isolated in Bali, Indonesia in 2016

Trend of HIV transmitted drug resistance before and after implementation of HAART regimen restriction in the treatment of HIV-1 infected patients in southern Taiwan

HIV-1 integrase strand-transfer inhibitor resistance in southern Taiwan

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