Prevalence and antimicrobial susceptibility pattern of methicillin-resistant, vancomycin-resistant, and Panton-Valentine leukocidin positive Staphylococcus aureus in a tertiary care hospital Dhaka, Bangladesh

Islam, Tashmin Afroz Binte; Shamsuzzaman, SM

doi:10.1016/j.tcmj.2014.12.001

Cited by 16 publications

(13 citation statements)

References 23 publications

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“…The overall prevalence rate of Staphylococcus aureus was 28.95% which is commensurate to previous reports [13], but higher than the rates observed in India [14,15], Bangladesh [16], and Yaoundé [7]. In contrast, higher rates of prevalence were reported in Nigeria [17], Ethiopia [18,19,20,21], Congo [22], and Iran [23,24].…”

Section: Discussionsupporting

confidence: 78%

Prevalence and Antibiotic Susceptibility Patterns of Methicillin Resistant Staphylococcus Aureus in Patients Attending the Laquintinie Hospital Douala, Cameroon

Bissong¹,

Wirgham²,

Enekegbe³

et al. 2016

EJCBS

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Methicillin Resistant Staphylococcus aureus (MRSA) is a major health problem worldwide. Diseases caused by these microorganisms most often are difficult to treat consequently, present with severe outcomes. Despite the increase association of MRSA in most hospital acquired infections, surveillance systems to control the spread of these infections are lacking especially in the developing countries. The present study is part of a major study developed to trail the incidence of multidrug resistant pathogens in clinical settings using available resources, with the aim to gather relevant data for the management and control of nosocomial infections. The specific objective of the current study is therefore to determine the prevalence and antimicrobial susceptibility of MRSA isolates from one of the largest hospitals in the country. This was a hospital based cross sectional study involving 114 participants, conducted between March and June 2016. Various clinical specimens were inoculated on to blood agar and mannitol salt agar and incubated at 35-37°C aerobically for 18-24 hours for the isolation of S. aureus. Isolates were identified using the catalase and coagulase tests. Sensitivity testing was done using the Kirby Bauer disk diffusion method. Data were analysed using SPSS version 20.0. Out of 114 samples analysed, S. aureus was isolated from 33 (28.95%) while MRSA was detected in 15 (13.16%). Antimicrobial susceptibility testing revealed high resistance (80-100%) to penicillins and no resistance to amikacin, lincomycin and ofloxacin. Multidrug resistance to at least three antibiotics was also observed among MRSA isolates. MRSA and multidrug resistant S. aureus are common in the study site. Therefore, there is a need for the improvement of surveillance systems to monitor and curb the spread of these resistant pathogens.

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Section: Discussionsupporting

confidence: 78%

Prevalence and Antibiotic Susceptibility Patterns of Methicillin Resistant Staphylococcus Aureus in Patients Attending the Laquintinie Hospital Douala, Cameroon

Bissong¹,

Wirgham²,

Enekegbe³

et al. 2016

EJCBS

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“…Thus, lack of policies on the regulation of drug acquisition and use of cocktail of drug preparations may be contributing to the antimicrobial resistance observed in this study and other studies in Africa and Asia. Similar reports of resistance to these antibiotics have been previously reported in both human and veterinary settings as well as from retail meat products in developing countries like Ghana [48,80,81], South Africa [82] and Bangladesh [83].…”

Section: Source (N)supporting

confidence: 82%

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers

et al. 2020

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The epidemiology of Staphylococcus aureus in food animals, associated products, and their zoonotic potential in Nigeria are poorly understood. This study aimed to provide data on the prevalence, genetic characteristics and antimicrobial resistance of S. aureus isolated from chicken and pig carcasses, and persons in contact with the carcasses at slaughterhouses in Nigeria. Surface swabs were collected randomly from 600 chicken and 600 pig carcasses. Nasal swabs were collected from 45 workers in chicken slaughterhouses and 45 pig slaughterhouse workers. S. aureus isolates were analyzed by spa typing. They were also examined for presence of the Panton-Valentine Leucocidin (PVL) and mecA genes, as well as for antimicrobial resistance phenotype. Overall, 53 S. aureus isolates were recovered (28 from chicken carcasses, 17 from pig carcasses, 5 from chicken carcass handlers and 3 from pig carcass handlers). Among the isolates, 19 (35.8%) were PVL-positive and 12 (22.6%) carried the mecA gene. The 53 isolates belonged to 19 spa types. The Based Upon Repeat Pattern (BURP) algorithm separated the isolates into 2 spa-clonal complexes (spa-CC) and 9 singletons including 2 novel spa types (t18345 and t18346). The clonal complexes (CC) detected were CC1, CC5, CC8, CC15, CC88 and CC152. CC15-related isolates represented by spa type t084 (32.1%) and CC5 represented by spa type t311 (35.3%) predominated among isolates from chicken carcasses/ handlers, and pig carcasses/ handlers, respectively. Multidrug resistance exhibited by all the CC except CC8, was observed among isolates from chicken carcasses (64.3%), pig carcasses (41.2%), handlers of chicken meat (40.0%) and handlers of pork (33.3%). All the CC showed varying degrees of resistance to tetracycline while CC15 and CC5 exhibited the highest resistance to sulphamethoxazole/trimethoprim and erythromycin, respectively. The predominant antimicrobial resistance

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“…All isolates showing resistance to oxacillin were subjected to polymerase chain reaction for the detection of mecA gene specific for MRSA molecular detection as described previously by [26]; mecA gene was amplified using the following primer sequence: mecA-F:50-AAAATCGATGGTAAAGGTTGGC-30 and mecA-R:50-AGTTCTGCAGTACCGGATTTTGC-30. From the previously identified MRSA BHI slants, a loopful from each culture was inoculated into BHI broth followed by incubation at 37 °C for 24 h. The genomic DNA was extracted using DNA Purification Kit (Qiagen) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

MIC and MBC of Honey and Gold Nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) coagulase-positive S. aureus isolated from contagious bovine clinical mastitis

Omara

2017

Journal of Genetic Engineering and Biotechnology

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Staphylococcus aureus is one of the major causative agents of the bovine clinical mastitis. This study aimed to isolate and identify S. aureus from cases of bovine clinical mastitis followed by phenotypic detection of MRSA and VRSA. The genotypic detection of MRSA was done through PCR detection of the resistance mecA gene. Furthermore, this study aimed to investigate the in vitro MIC and MBC of the Dodonaea angustifolia plant extract, Honey, and AuNPs against the clinically isolated MRSA and VRSA. Of 93 mastitis milk samples examined, 54 (58.1%) S. aureus were isolated and identified {CP S. aureus = 46 (85.2%) and CN S. aureus = 8 (14.8%)}. The whole MRSA, VRSA, MSSA, and VSSA detected were 19 (35.2%), 7 (13%), 35 (65%), and 47 (87%) respectively. The mean counts of S. aureus were between 8.6 × 104 ± 3.5 × 105 CFU/ml. The oxacillin and vancomycin MICs against MRSA and VRSA respectively, were >256 µg/ml. AuNPs sized 30 nm produce observable in vitro anti-MRSA and anti-VRSA activities. Imtenan® citrus blossom honey has also antibacterial activities against MRSA and VRSA with general MBC and MIC range values were observed at a concentration of 0.625, 1.25, 2.5, and 5 (%v/v). In the present study, the most significant result obtained when AuNPs was mixed with Imtenan® citrus blossom honey (1:1 = v:v) with the best MBC was observed at the concentration of 0.56 × 109:0.3 (NP/ml: honey %v/v).

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Prevalence and antimicrobial susceptibility pattern of methicillin-resistant, vancomycin-resistant, and Panton-Valentine leukocidin positive Staphylococcus aureus in a tertiary care hospital Dhaka, Bangladesh

Cited by 16 publications

References 23 publications

Prevalence and Antibiotic Susceptibility Patterns of Methicillin Resistant Staphylococcus Aureus in Patients Attending the Laquintinie Hospital Douala, Cameroon

Prevalence and Antibiotic Susceptibility Patterns of Methicillin Resistant Staphylococcus Aureus in Patients Attending the Laquintinie Hospital Douala, Cameroon

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers

MIC and MBC of Honey and Gold Nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) coagulase-positive S. aureus isolated from contagious bovine clinical mastitis

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