Prevalence and antimicrobial susceptibility of gram-negative bacteria isolated from bovine mastitis between 2003 and 2008 in Korea

Nam, Hyang-Mi; Lim, Suk-Kyung; Kang, Hyun-Mi; Kim, J.M.; Moon, Jin-San; Jang, Keum-Chan; Joo, Yi-Seok; Jung, Suk-Chan

doi:10.3168/jds.2008-1739

Cited by 58 publications

(51 citation statements)

References 20 publications

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“…Similar results were found in cows with clinical mastitis and milk samples with mastitis in Korea (Nam et al 2009, Gurung et al 2013. The association of this bacterium with subclinical mastitis in buffaloes presents a new challenge for the treatment and control of disease.…”

Section: Discussionsupporting

confidence: 78%

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Vásquez-García

Silva²,

Almeida-Queiroz³

et al. 2017

Pesq. Vet. Bras.

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ABSTRACT.-Vásquez-García A., Silva T.S., Almeida-Queiroz S.R., Godoy S.H.S., Escherichia coli (4.5%), Serratia marcescens (4.0%), Stenotrophomonas maltophilia (4.0%), and low incidence: Klebsiella rhinoscleromatis (0.5%), Klebsiella ozaenae (0.5%), Tatumella ptyseos (0.5%), Enterobacter cloacae (0.5%). The molecular analysis indicated that samples positive by culture method of the genera Staphylococcus, Streptococcus and E. coli were positive by PCR. Para S. aureus and S. epidermidis the highest percentages of observed sensitivity were gentamicin (100%) and vancomycin (100%); for the genus Streptococcus to gentamicin and oxacillin and E. coli to Ampicilin. These findings may help in the control and treatment of subclinical mastitis in buffaloes and contribute to improving the efficiency and quality of the milk produced.

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Section: Discussionsupporting

confidence: 78%

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Vásquez-García

Silva²,

Almeida-Queiroz³

et al. 2017

Pesq. Vet. Bras.

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“…The herd prevalence of S. maltophilia was three (0·3%) of the approximately 1000 dairy herds in the Nemuro Subprefecture. In contrast, the incidence of S. maltophilia isolation was 17 (0·083%) per 20 386 mastitis milk samples in South Korea (Nam et al. 2009).…”

Section: Results Of Eric2‐pcr Typing and Mic (μG Ml−1) Values Of Sixmentioning

confidence: 98%

“…2010). However, S. maltophilia is rarely isolated from bovine mastitis (Nam et al. 2009), and its involvement in bovine mastitis remains unclear.…”

Section: Results Of Eric2‐pcr Typing and Mic (μG Ml−1) Values Of Sixmentioning

confidence: 99%

Antimicrobial susceptibility and genetic relatedness of bovine Stenotrophomonas maltophilia isolates from a mastitis outbreak

Ohnishi

Sawada

Marumo

et al. 2012

Letters in Applied Microbiology

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Aims: To evaluate the antimicrobial susceptibility and genetic relatedness of 11 Stenotrophomonas maltophilia isolates from an outbreak of bovine clinical mastitis in one herd and two isolates from two separate mastitis cases in two other herds. Methods and Results: Thirteen S. maltophilia isolates were obtained from milk samples from 11 cows from three dairy herds in Japan during 2008. We tested their susceptibility to 14 antimicrobials by broth microdilution and identified their genotypes by enterobacterial repetitive intergenic consensus 2 (ERIC2)‐PCR. Every cow had acute mild mastitis (slightly watery foremilk with flakes) without systemic symptoms and all resolved within 3–5 weeks of diagnosis. Eleven of the 13 isolates derived from nine cows in one herd over a 7‐month period exhibited a closely related ERIC2 type (A). The remaining two isolates derived from two cows from two other herds exhibited two distinct ERIC2 types (B and C). Most of the 13 isolates exhibited susceptibility to trimethoprim‐sulfamethoxazole, chloramphenicol, minocycline and levofloxacin; however, they were resistant to four β‐lactams, kanamycin, gentamicin and oxytetracycline. They were intermediate to enrofloxacin. Conclusions: Eleven closely related S. maltophilia isolates were involved in a herd outbreak of mastitis to some extent. Bovine S. maltophilia isolates exhibited resistance to many classes of antimicrobials. Significance and impact of study: This is a rare report of a herd outbreak of bovine mastitis involving closely related S. maltophilia isolates.

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“…Also, Sphingomonas paucimobilis has been linked to a variety of nosocomial infections in humans [61], [62]. S. paucimobilis and S. maltophilia were both detected in a dairy study performed over the course of six years that examined gram-negative bacteria in milk samples with elevated somatic cell counts [63].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Community Profiling of Milk Samples as a Means to Understand Culture-Negative Bovine Clinical Mastitis

et al. 2013

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Inflammation and infection of bovine mammary glands, commonly known as mastitis, imposes significant losses each year in the dairy industry worldwide. While several different bacterial species have been identified as causative agents of mastitis, many clinical mastitis cases remain culture negative, even after enrichment for bacterial growth. To understand the basis for this increasingly common phenomenon, the composition of bacterial communities from milk samples was analyzed using culture independent pyrosequencing of amplicons of 16S ribosomal RNA genes (16S rDNA). Comparisons were made of the microbial community composition of culture negative milk samples from mastitic quarters with that of non-mastitic quarters from the same animals. Genomic DNA from culture-negative clinical and healthy quarter sample pairs was isolated, and amplicon libraries were prepared using indexed primers specific to the V1–V2 region of bacterial 16S rRNA genes and sequenced using the Roche 454 GS FLX with titanium chemistry. Evaluation of the taxonomic composition of these samples revealed significant differences in the microbiota in milk from mastitic and healthy quarters. Statistical analysis identified seven bacterial genera that may be mainly responsible for the observed microbial community differences between mastitic and healthy quarters. Collectively, these results provide evidence that cases of culture negative mastitis can be associated with bacterial species that may be present below culture detection thresholds used here. The application of culture-independent bacterial community profiling represents a powerful approach to understand long-standing questions in animal health and disease.

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Prevalence and antimicrobial susceptibility of gram-negative bacteria isolated from bovine mastitis between 2003 and 2008 in Korea

Cited by 58 publications

References 20 publications

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Antimicrobial susceptibility and genetic relatedness of bovine Stenotrophomonas maltophilia isolates from a mastitis outbreak

Bacterial Community Profiling of Milk Samples as a Means to Understand Culture-Negative Bovine Clinical Mastitis

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