Antimicrobial susceptibility and genetic relatedness of bovine Stenotrophomonas maltophilia isolates from a mastitis outbreak

Ohnishi, Mamoru; Sawada, Takuo; Marumo, Kenji; Harada, Kazuki; Hirose, Kazuhiko; Shimizu, Akira; Hayashimoto, Mizuki; Sato, Reiichiro; Uchida, Naoki; Kato, Harubumi

doi:10.1111/j.1472-765x.2012.03246.x

Cited by 27 publications

(19 citation statements)

References 12 publications

(38 reference statements)

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“…Both have been previously detected in dairy environments in France [52]. Stenotrophomonas maltophilia has been reported in association with an outbreak of clinical mastitis in cattle in Japan [53]. In that study the bacteria were readily cultured from milk using a method very similar to that used for this work, suggesting that the Stenotrophomonas sequences identified here may be from a species other than S. maltophilia .…”

Section: Discussionmentioning

confidence: 52%

Bacterial Community Profiling of Milk Samples as a Means to Understand Culture-Negative Bovine Clinical Mastitis

et al. 2013

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Inflammation and infection of bovine mammary glands, commonly known as mastitis, imposes significant losses each year in the dairy industry worldwide. While several different bacterial species have been identified as causative agents of mastitis, many clinical mastitis cases remain culture negative, even after enrichment for bacterial growth. To understand the basis for this increasingly common phenomenon, the composition of bacterial communities from milk samples was analyzed using culture independent pyrosequencing of amplicons of 16S ribosomal RNA genes (16S rDNA). Comparisons were made of the microbial community composition of culture negative milk samples from mastitic quarters with that of non-mastitic quarters from the same animals. Genomic DNA from culture-negative clinical and healthy quarter sample pairs was isolated, and amplicon libraries were prepared using indexed primers specific to the V1–V2 region of bacterial 16S rRNA genes and sequenced using the Roche 454 GS FLX with titanium chemistry. Evaluation of the taxonomic composition of these samples revealed significant differences in the microbiota in milk from mastitic and healthy quarters. Statistical analysis identified seven bacterial genera that may be mainly responsible for the observed microbial community differences between mastitic and healthy quarters. Collectively, these results provide evidence that cases of culture negative mastitis can be associated with bacterial species that may be present below culture detection thresholds used here. The application of culture-independent bacterial community profiling represents a powerful approach to understand long-standing questions in animal health and disease.

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Section: Discussionmentioning

confidence: 52%

Bacterial Community Profiling of Milk Samples as a Means to Understand Culture-Negative Bovine Clinical Mastitis

et al. 2013

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“…Microbiota dominance and decreased heterogeneity are proposed characteristics of mastitis-associated dysbiosis (Oikonomou et al, 2014). Stenotrophomonas spp., in particular S. maltophilia, have been occasionally isolated from mastitic quarters (Nam et al, 2009;Ohnishi et al, 2012). Predominance of Stenotrophomonas has been also reported within the milk microbiota of culture-negative mastitis samples (Kuehn et al, 2013).…”

Section: Shifts In Diversity and Composition Of Udder Microbiotamentioning

confidence: 95%

Composition of the teat canal and intramammary microbiota of dairy cows subjected to antimicrobial dry cow therapy and internal teat sealant

Derakhshani

Plaizier

Buck

et al. 2018

Journal of Dairy Science

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Antimicrobial dry cow therapy (DCT) is an important component of mastitis control programs aimed to eliminate existing intramammary infections and prevent the development of new ones during the dry period. However, to what extent the microbiota profiles of different niches of the udder change during the dry period and following administration of DCT remains poorly understood. Therefore, the main objective of the present study was to qualitatively evaluate dynamics of the microbiota of teat canal (TC) and mammary secretions (i.e., milk and colostrum) of healthy udder quarters subjected to DCT using a long-acting antimicrobial product, containing penicillin G and novobiocin, in combination with internal teat sealant. To this end, TC swabs (n = 58) and their corresponding milk (n = 29) and colostrum samples (n = 29) were collected at the time of drying off and immediately after calving from clinically healthy udder quarters of Holstein dairy cows from a commercial dairy farm. All samples were subjected to DNA extraction and high-throughput sequencing of the V1-V2 hypervariable regions of bacterial 16S rRNA genes. Overall, shifts were more pronounced within the microbiota of mammary secretions than the TC. In particular, microbiota of colostrum samples collected immediately after calving were less species-rich compared with the pre-DCT milk samples. Proportions of several bacterial genera belonging to the phylum Proteobacteria, including Pseudomonas, Stenotrophomonas, and unclassified Alcaligenaceae, were enriched within the microbiota of colostrum samples, whereas Firmicutes genera, including Butyrivibrio, unclassified Clostridiaceae, and unclassified Bacillales, were overrepresented in pre-DCT milk microbiota. Apart from shifts in the proportion of main bacterial genera and phyla, qualitative analysis revealed a high degree of commonality between pre-DCT and postpartum microbiota of both niches of the udder. Most importantly, a considerable number of bacterial genera and species commonly regarded as mastitis pathogens or opportunists (or both), including Staphylococcus spp., unclassified Enterobacteriaceae, and Corynebacterium spp., were shared between pre-DCT and postpartum microbiota of mammary secretions. Percentage of shared bacterial genera and species was even higher between pre-DCT and postpartum microbiota of TC samples, suggesting that the DCT approach of the present study had limited success in eliminating a considerable proportion of bacteria during the dry period.

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“…A previous study indicated that the closely related S. maltophilia isolates were involved in a herd outbreak of mild mastitis in cattle in Japan. 26 Stenotrophomonas maltophilia has also been found to be environmental global emerging Gram-negative bacterial pathogen that can cause various serious infections in humans. 27 Owing to their multi-antibiotic resistance, 28 infections or diseases caused by S. maltophilia would be exceptionally difficult to treat.…”

Section: Influence Of Hc Feeding On Changes In the Composition Of Milmentioning

confidence: 99%

Characterization of bacterial community of raw milk from dairy cows during subacute ruminal acidosis challenge by high-throughput sequencing

Zhang

Huo

Zhu

et al. 2014

J. Sci. Food Agric.

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Antimicrobial susceptibility and genetic relatedness of bovine Stenotrophomonas maltophilia isolates from a mastitis outbreak

Cited by 27 publications

References 12 publications

Bacterial Community Profiling of Milk Samples as a Means to Understand Culture-Negative Bovine Clinical Mastitis

Bacterial Community Profiling of Milk Samples as a Means to Understand Culture-Negative Bovine Clinical Mastitis

Composition of the teat canal and intramammary microbiota of dairy cows subjected to antimicrobial dry cow therapy and internal teat sealant

Characterization of bacterial community of raw milk from dairy cows during subacute ruminal acidosis challenge by high-throughput sequencing

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