Prevalence and antimicrobial resistance of Campylobacter isolated from retail raw chicken and beef meat, Tehran, Iran

Taremi, Mahnaz; Dallal, Mohammad Mehdi Soltan; Gachkar, Latif; MoezArdalan, Sanaz; Zolfagharian, Koorosh; Zali, Mohammad Reza

doi:10.1016/j.ijfoodmicro.2005.12.010

Cited by 43 publications

(55 citation statements)

References 26 publications

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“…Such reports, from both developed and developing countries, reveal that poultry meat is the food vehicle most frequently contaminated with Campylobacter spp. [27]. These findings, along with the findings in this study are suggestive of high prevalence of Campylobacter at flocks level as previously reported by Stern et al [28] and Arsenault et al [29] who observed a positive correlation between the contamination of carcasses and the high positivity rates for Campylobacter of flocks at the farm level.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Contamination of Frozen Broiler Chicken Meats with Antimicrobial Resistant Thermophilic Campylobacter in Morogoro, Tanzania

Msetule¹,

Evg²,

Si³

et al. 2017

BJSTR

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Purpose: Majority of the human bacterial gastroenteritis cases in both developed and developing countries in the world are caused by thermo tolerant Campylobacter spp, with C. jejuni and C. coli being more involved. These bacteria live in poultry and other animal's intestinal tracts without causing disease symptoms. Improper handling of carcasses during slaughter and evisceration increases the chance of contaminating the outer skin. Poor meat handling in the kitchen and poor storage during refrigeration causes cross contamination. Methods:This cross-sectional study examined the occurrence of antimicrobial resistant thermophilic Campylobacter in frozen chicken meats in Morogoro Municipality. A total of 272 frozen broiler chicken carcasses were obtained from supermarkets (n=90), meat shops (n=90) and restaurants (n=92). Each carcass was divided into three parts i.e. breast, thighs and wing+neck, rinsed in peptone water. The rinses were then enriched in 5ml of Bolton broth in microerophilic atmosphere at 42ºC for 24hrs. Then the broth was streaked on Modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) plates followed by incubation at 42ºC for 48 hrs. Presumptive colonies of Campylobacter spp. were sub cultured onto Mueller Hinton Agar and incubated at 42ºC for 48 hrs to obtain pure colonies. Pure Campylobacter colonies were then subjected to preliminary identification using biochemical tests and further confirmed by polymerase chain reaction (PCR). Antimicrobial Susceptibility testing was performed on C. Jejuni isolates by disc diffusion method on Mueller-Hinton agar supplemented with 5% of horse blood. The isolates were tested for resistance against ten antimicrobials namely tetracycline, gentamycin, ciprofloxacin, azithromycin, erythromycin, norfloxacin, chloramphenical, amoxicilin, nalidixic acid and cephalocin.Results: Occurrence of thermophilic Campylobacter spp. in the sampled birds was at a tune of 61.0%. Based on sampling locations the levels of contaminated chicken carcasses were 82.6%, 62.0% and 37.8% for restaurants, shops and supermarkets, respectively. Majority of isolates (72.4%) were Campylobacter jejuni and the remaining proportion (27.6%) was accounted for by C. coli. Frequencies of contamination were comparable between wing+neck and thighs. Wing+neck and thighs had significantly higher frequencies of contamination than breasts (P<0.05). Antibiotic resistance test results for C. jejuni indicated that higher levels (>80%) of resistance were observed for cephalothin, chloramphenical, nalidixic acid, amoxicillin and tetracycline. Lower levels of resistance (<15%) were observed for erythromycin, norfloxacin, azithromycin, ciprofloxacin and gentamicin. Conclusion:This study confirms that in our setting a high frequency of commercial broiler chickens are positive for antimicrobial resistant Campylobacter at the time of slaughter. This phenomenon derives in high contamination of carcasses during the slaughter process thereby constituting a substantial public health hazard. Freezing of carcass...

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Section: Discussionsupporting

confidence: 91%

“…The obtained prevalence of frozen chicken contamination in this study (61.0%) falls within this stated range. More or less similar contamination prevalence's have been reported in Brazil 62.2% [34] and in Iran 63.0%; [27]. Low prevalence's of retail poultry contamination with Campylobacter spp.…”

Section: Discussionsupporting

confidence: 65%

Contamination of Frozen Broiler Chicken Meats with Antimicrobial Resistant Thermophilic Campylobacter in Morogoro, Tanzania

Msetule¹,

Evg²,

Si³

et al. 2017

BJSTR

View full text Add to dashboard Cite

show abstract

“…Where they are reported, rates of resistance to antibiotics of bacteria originating from meat were high in developing countries (2,3,16,37,54), possibly as the result of the inappropriate or uncontrolled use of antibiotics in farming practices. Therefore, the study of antibiotic resistance in developing countries is important as the information could enhance prudent use of antibiotics in food production.…”

mentioning

confidence: 99%

Antibiotic Resistance in Food-Borne Bacterial Contaminants in Vietnam

Van

Moutafis

Tran

et al. 2007

Appl Environ Microbiol

109

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This study was conducted to examine the rate of contamination and the molecular characteristics of enteric bacteria isolated from a selection of food sources in Vietnam. One hundred eighty raw food samples were tested; 60.8% of meat samples and 18.0% of shellfish samples were contaminated with Salmonella spp., and more than 90% of all food sources contained Escherichia coli. The isolates were screened for antibiotic resistance against 15 antibiotics, and 50.5% of Salmonella isolates and 83.8% of E. coli isolates were resistant to at least one antibiotic. Isolates were examined for the presence of mobile genetic elements conferring antibiotic resistance. Fifty-seven percent of E. coli and 13% of Salmonella isolates were found to contain integrons, and some isolates contained two integrons. Sequencing results revealed that the integrons harbored various gene cassettes, including aadA1, aadA2, and aadA5 (resistance to streptomycin and spectinomycin), aacA4 (resistance to aminoglycosides), the dihydrofolate reductase gene cassettes dhfrXII, dfrA1, and dhfrA17 (trimethoprim resistance), the beta-lactamase gene bla PSE1 (ampicillin resistance), and catB3 (chloramphenicol resistance). Plasmids were also detected in all 23 antibiotic-resistant Salmonella isolates and in 33 E. coli isolates. Thirty-five percent of the Salmonella isolates and 76% of the E. coli isolates contained plasmids of more than 95 kb, and some of the isolates contained two large plasmids. Conjugation experiments showed the successful transfer of all or part of the antibiotic resistance phenotypes among the Salmonella and E. coli food isolates. Our results show that enteric bacteria in raw food samples from Vietnam contain a pool of mobile genetic elements and that the transfer of antibiotic resistance can readily occur between similar bacteria.

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“…The rates of isolation of Campylobacter contamination are higher in countries with traditional "pluck shop" based wet markets: 63% in Iran (Taremi et al, 2006), 68.3% in Korea (Han et al, 2007) and 83.9% in Trinidad. Studies in Malaysia have shown the overall rate of contamination for Campylobacter in modern processing plants and in traditional wet markets were 61.1 and 85.6%, respectively (Rejab et al, 2012).…”

Section: Prevalence Of Campylobacter In Retail Poultry Meat From "Plumentioning

confidence: 98%

Prevalence, seasonality and antibiotic susceptibility of thermophilic Campylobacters in ceca and carcasses of poultry birds in the live-bird market

Parkar¹,

Sachdev²,

deSouza³

et al. 2013

Afr. J. Microbiol. Res.

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Prevalence and antimicrobial resistance of Campylobacter isolated from retail raw chicken and beef meat, Tehran, Iran

Cited by 43 publications

References 26 publications

Contamination of Frozen Broiler Chicken Meats with Antimicrobial Resistant Thermophilic Campylobacter in Morogoro, Tanzania

Contamination of Frozen Broiler Chicken Meats with Antimicrobial Resistant Thermophilic Campylobacter in Morogoro, Tanzania

Antibiotic Resistance in Food-Borne Bacterial Contaminants in Vietnam

Prevalence, seasonality and antibiotic susceptibility of thermophilic Campylobacters in ceca and carcasses of poultry birds in the live-bird market

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