Prevalence and antimicrobial resistance of Listeria species and subtyping and virulence factors of Listeria monocytogenes from retail meat

Abstract

In this study, of 124 samples of ground beef and chicken meat, 101 (81.5%) were positive for Listeria spp. Listeria innocua (35.5%) and Listeria monocytogenes (26.6%) were the most frequently isolated species. All 33 (100%) L. monocytogenes isolates were positive for the virulence genes hlyA, actA, inlA, inlB, inlC, inlJ, prfA, plcA, and iap. Serotype 1/2a, which is one of the listeriosis‐associated serotypes, was the most predominant serotype (57.6%), followed by 1/2c (39.4%) and 3c (3%). In polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis of inlA for subtyping of the L. monocytogenes strains, the AluI and MluCI enzymes generated two different profiles. Almost all chicken strains were grouped in similar PCR‐RFLP profiles with both restriction enzymes. Most Listeria isolates were resistant to ceftriaxone (79.7%), fusidic acid (63.3%), and clindamycin (47.7%). Resistance to penicillin and ampicillin occurred in 1.6 and 17.9% of the isolates, respectively. The percentage of resistance to three or more agents was 48.1%. Practical application The presence of Listeria spp., especially L. monocytogenes, in foods is inevitable because of the ubiquitous nature of Listeria spp. Illnesses resulting from the consumption of contaminated food severely affect sensitive groups, especially pregnant women, unborn fetuses and newborns as a result of transmission across the placenta or during delivery. Immunosuppressed adults are also sensitive to infection. Listeria grows well at cold temperatures, so storage of contaminated food in the refrigerator can also increase the risk of infections for consumers. In particular, limiting the exposure of pregnant women and immunosuppressed patients to potential sources of Listeria such as raw and undercooked meats is recommended.

Detection of Listeria species, factors associated, and antibiogram of Listeria monocytogenes in beef at abattoirs, butchers, and restaurants of Ambo and Holeta Towns, Ethiopia

“…In the present study, Listeria species was isolated from 28.44% of raw beef samples from abattoir, butcher, and restaurants which agrees with the reports from Addis Ababa (27.5%) [3]. Other studies have reported signi cantly higher isolation rates of Listeria species in raw meat such as 95% in Brazil [26], 81.5% in Turkey [27], 58% in Nigeria [28], 54.1% in Turkey [29], 51.3% in Ethiopia [21], and 50% in Jordan [30].…”

“…However, the current nding was higher when compared to the 1.29% isolation rate from raw cow and goat meats [31], 1.6% from minced beef [20] and 2.5% from bovine carcasses in Poland [32]. The present nding of L. monocytogenes (4.4%) was lower when compared to the 15.6% [33] in Nordic countries,15.4% in Bangkok [34], 25.5% in Turkey [27]. The relatively low prevalence of L. monocytogenes in present study might be attributed to the difference in the study season, geographic conditions, and sample size [35,36].…”

Antimicrobial Susceptibility and Multilocus Sequence Typing ofListeria monocytogenesIsolated Over 11 Years from Food, Humans, and the Environment in Italy

“…However, Godreuil et al (2003) reported that only five of 488 clinical isolates of L. monocytogenes were susceptible to chloramphenicol and vancomycin (Walsh et al, 2001). Although L. monocytogenes was noted to be relatively susceptible to a wide range of antimicrobials as few as 20 years ago, a number of more recent reports suggests that the rate of antimicrobial resistance in L. monocytogenes is increasing from 1% to 10% up to 50% (Vela et al, 2001;Walsh et al, 2001;Godreuil et al, 2003;Barbosa et al, 2013;Gómez et al, 2014;Thønnings et al, 2016;Arslan et al, 2019). Accordingly, in the present study, a median increase per year of the percentage of antimicrobial resistant or reduced susceptible isolates to oxacillin, clindamycin, and ciprofloxacin was observed in the period 2007-2009 in comparison to previous years (1998-2006).…”

Organik Tavuk Etlerinden İzole Edilen Listeria monocytogenes İzolatlarının Serotip ve Antibiyotik Direnç Profilinin Belirlenmesi

“…In other studies, Carvalho et al [29] reported mostly 1/2a (94.6%) in chicken meat and chicken-meat processing environment, Oliveira et al [30] identified 87% of the L. monocytogenes isolates as 1/2a in samples of chicken carcasses, and Zeinali et al [31] determined that 52.77% of L. monocytogenes 1/2a serotype were predominant in chicken carcasses, followed by 4a and 4c serotypes (27.77%) in İran. Arslan and Baytur [32] revealed 57.6% of L. monocytogenes strains isolated from chicken meat were 1/2a. In contrast, Zeinali et al [33] and Maung et al [34] reported 1/2b dominance in samples of chicken meats.…”