Prevalence and antimicrobial resistance of Salmonella in chicken carcasses at retail in 15 Brazilian cities

Medeiros, Marcelo Augusto Nunes; Oliveira, Diana; Rodrigues, Dália dos Prazeres; Freitas, Daniel Roberto Coradi de

doi:10.1590/s1020-49892011001200010

Cited by 131 publications

(122 citation statements)

References 21 publications

(24 reference statements)

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“…Interestingly, the overall incidence level of Salmonella (17%) from broilers was close to that (21.99%) reported earlier in Bangladesh [22]. Many studies showed different prevalence rates of Salmonella isolates in broilers worldwide: in Brazil, a low rate of 2.7% was reported [23], while in China, a high rate of 52.2% was reported [3]. These differences in prevalence rates may reflect considerable disparity in the sampling scheme, sample types, Salmonella detection protocol, and geographic location.…”

Section: Discussionsupporting

confidence: 79%

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Ammar

Mohamed

El-Hamid

et al. 2016

J Infect Dev Ctries

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Introduction: Salmonella serovars are one of the primary foodborne pathogens. Poultry consumption is responsible for the majority of disease cases worldwide. The prevalence of virulence determinants among Salmonella serovars appears to be lacking in Egypt. Therefore, this study investigated the occurrence, antibiotic resistance patterns, and virulence gene profiling of Salmonella serovars in broilers. Methodology: Three hundred samples from broiler chickens were examined for the presence of Salmonella by standard microbiological techniques. All Salmonella isolates were tested for their sensitivity against ten antibiotics and subjected to virulence genotyping by polymerase chain reaction (PCR). Results: The overall isolation percentage of Salmonella was 17%. Seven different serovars were found, with the main one being Salmonella Typhimurium (52.94%). Salmonella isolates were sensitive to most of the tested antibiotics, but they exhibited absolute resistance against amoxicillin/clavulanic acid. Nine Salmonella strains (52.94%) were resistant to at least three antibiotics. Further PCR investigations into 17 Salmonella strains revealed different distribution patterns of eight virulence determinants among the isolates. The invA gene was the most prevalent one (100%), followed by hilA (88.24%), stn (58.82%), and fliC genes (52.94%), while each of sopB and pefA genes had a similar prevalence (41.18%), and sefC and spvC genes had the lowest prevalence (11.76 and 5.88%, respectively). PCR genotyping allowed grouping of Salmonella strains into ten genetic profiles. Conclusions: These results will help in understanding the spread of virulence genotypes and antibiotic resistance among Salmonella serovars in broilers.

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Section: Discussionsupporting

confidence: 79%

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Ammar

Mohamed

El-Hamid

et al. 2016

J Infect Dev Ctries

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“…Also, the result of Cardoso et al (2006) mentions, besides ciprofloxacin, the susceptibility to gentamicin, norfloxacin and sulfazotrin of strains isolated from chicken carcasses in the state of Rio Grande do Sul is similar to our results. Medeiros et al (2011), researching the resistance of 18 Salmonella serotypes isolated from frozen broiler carcasses in 15 Brazilian cities against antimicrobials, found that strains of S. enteritidis were resistant to all antibiotics tested, at various levels, including antimicrobials used in this study. Also, the highest levels of bacterial resistance to antimicrobial agents were assigned to Streptomycin, florfenicol, sulfonamide, nalidixic acid, ampicillin, ceftiofur, aztreonam, cefoxitine, cephalotin, gentamicin and tetracycline, partially corroborating with our results.…”

Section: Discussionmentioning

confidence: 72%

Occurrence and antimicrobial resistance of Salmonella serotypes isolates recovered from poultry of Western Paran, Brazil

Scur¹,

Pinto²,

Bona³

et al. 2014

Afr. J. Agric. Res.

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This study was conducted to determine the occurrence and antimicrobial resistance of Salmonella serotypes isolates from feces, feed and broiler litter in poultry broilers in western Paraná between 2006 and 2010. The antigenic characterization and identification of serotypes confirmed 118 samples of Salmonella spp., belonging to 38 different serotypes. The most common serotypes identified were S. enteritidis (16.1%), S. heidelberg (5.9%), S. typhimurium (5.9%), S. hadar (5.0%), S. albany (4.2%), S. enterica (4.2) and S. saintpaul (4.2%). The serotypes of Salmonella spp. isolates were examined for resistance against 13 antibiotics using the disc diffusion method. Thirty-six samples (30.5%) were susceptible to all antimicrobials, and among the 82 resistant strains, there were 25 different resistance patterns. Serotypes' multi-resistant to streptomycin, nalidixic acid and tetracycline (20.7%), nalidixic acid (13.4%) and nalidixic acid and tetracycline (11.0%) were found at higher frequency. The results revealed high prevalence of Salmonella spp. mainly S. enteritidis (16.1%) in aviary environment and percentage of strains' resistant to conventional antibiotics.

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“…Moreover, these SH isolates showed MDRI above 0.3, indicating a multidrug resistance profile and a great potential for horizontal transmission of resistance genes to other hosts. High IRMA indicates a risk to public health and may hinder the treatment of animal and human diseases, warning for the need of rational use of these drugs [10].…”

Section: Discussionmentioning

confidence: 99%

Salmonella Heidelberg Isolated from Poultry Shows a Novel Resistance Profile

Neves

Stefani

Pick

et al. 2016

Acta Scientiae. Vet.

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Background:The development of antimicrobial resistance in bacteria is a serious public health issue worldwide. Salmonella spp. is considered a leader cause of gastrointestinal disease in animals and humans, and poultry products have been reported as an important reservoir of the bacterium. S. Heidelberg became lately one of the most prevalent serovars found in several countries. However, hardly any information is available about the epidemiology and the resistance profile of it. Therefore, the objective of this study was to determine the antibiotic resistance profile of S. Heidelberg (SH) and to compare to S. Enteritidis (SE) and S. Typhimurium (ST) isolated from the Southern part of Brazil. Materials, Methods & Results:A total of 162 Salmonella isolates of poultry origin serotyped as SH (54), SE (54), and ST (54) were submitted to the disk-diffusion test with disks containing 10 antibiotics of 7 different classes, routinely used in veterinary and human medicine such as: enrofloxacin, ciprofloxacin, norfloxacin, gentamicin, ceftiofur, ceftriaxone, nalidixic acid, tetracycline, sulfamethoxazole-trimethoprim, and chloramphenicol. In addition, the Multi-drug Resistance Pattern (MDRP) and the Multiple-Drug Resistance Index (MDRI) were determined. The Chi-square (χ 2 ) test with 1% of significance level was used to statistically evaluate the results. All isolates were susceptible to norfloxacin, enrofloxacin, and ciprofloxacin. The majority of the isolates were resistant to the quinolone class (68%), more specifically to nalidixic acid, which is considered a synthetic quinolone, followed by penicillin (47%) and cephalosporin (16%). Overall, SH isolates showed higher resistance compared to ST and SE (18,16.5, and 9.6%, respectively). SH isolated from field samples, mainly drag swabs, showed higher resistance levels (24.2%) than those isolated from slaughterhouses (5.6%). SH showed the highest percentage of resistance to ceftiofur (31.5%), ceftriaxone (9.3%), and tetracycline (64.8%) in comparison to the other two serotypes. Most of the SH isolates were resistant to at least two (66.7%), three or more antibiotics (33.3%). A different scenario was observed for ST and SE, where 25.9 and 9.3% were susceptible to at least one drug, respectively. The most common pattern of resistance (MDRP) was C (gentamicin -nalidixic acid -tetracycline) for 14 SH isolates, and A (ceftiofur -nalidixic acid -tetracycline) for 12 SH isolates. MDRI indicated that 22.8% of all isolates were multidrug resistant. SH was the isolate with the largest variety of resistance patterns compared to ST and SE, where 11.7% of the isolates were resistant to more than three antibiotics. In addition, SH showed the greatest MDRI (0.25) ranging from 0.2 to 0.5. Discussion: SH was resistant to almost all antibiotics tested and showed multi-drug resistant profile, therefores, it showed a potential for horizontal transmission of resistance genes. Additionally, SH showed a higher resistance profile for ceftiofur, an important antibiotic used in poultry, w...

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Prevalence and antimicrobial resistance of Salmonella in chicken carcasses at retail in 15 Brazilian cities

Cited by 131 publications

References 21 publications

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Occurrence and antimicrobial resistance of Salmonella serotypes isolates recovered from poultry of Western Paran, Brazil

Salmonella Heidelberg Isolated from Poultry Shows a Novel Resistance Profile

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