Prevalence and antibiotic resistance of Staphylococcus strains isolated from meat products sold in Abidjan streets (Ivory Coast)

Abstract

Our study aimed to carry out the prevalence and antibiotic resistance of the coagulase positive and negative Staphylococcus isolated from meat product sold in streets in Abidjan (Ivory Coast). Two hundred and forty (240) samples from three kind of meat product (beef, pork and chickens) were collected in four popular communes (Abobo, Adjamé, Treichville and Yopougon) of Abidjan. These samples were composed of 80 samples of each kind of meat. After seeding on appropriate medium, suspected Staphylococcus strains were preliminary identified using API Staph protocol. The exact bacteria identity was confirmed by MALDI-TOF mass spectrometry. The Staphylococcus strains susceptibility to 18 antibiotics was determined using the disc diffusion method on Mueller Hinton medium. Out of the 240 tested samples, 96 Staphylococcus strains were isolated and identified. The coagulase positive specie isolated was Staphylococcus aureus with 19/96 (19.79%). Among the 77 coagulase negative strains, S. sciuri (32/77) was the most isolated followed by S. simulans (15/77). The highest resistance level was observed with erythromycin (100% for coagulase positive and 69.5% for coagulase negative). None resistance was observed with imipenem. The observed resistance to antibiotics of Staphylococcus strains suggests that the streets meat products sold at Abidjan are not appropriate and can be able to present a public health danger for the consumer.

Safety of ready‐to‐eat chicken in Burkina Faso: Microbiological quality, antibiotic resistance, and virulence genes in Escherichia coli isolated from chicken samples of Ouagadougou

“…Consequently, the consumption of these contaminated products could be a public health risk for consumers. Previous studies in West Africa have showed contamination of fresh chicken meat and offals by AMBs and TTCs (Attien et al., ; Ilboudo, Savadogo, Samandoulougou, & Abre, ). Otherwise, these studies have shown that the conditions for slaughter, scalding, evisceration, plucking, bleeding, washing, rinsing, preserving, grilling, and selling may be the ways of contamination.…”

“…No Salmonella and Staphylococcus coagulase positive were found in this study. However, others studies notified the presence of these bacteria in fresh poultry meat (Attien et al., ; Kagambèga et al., ; Khallaf et al., ; Nzouankeu, Ngandjio, Ejenguele, Njine, & Wouafo, ).…”

Molecular detection of antibiotic resistance genes in identified of Coagulase Negative Staphylococci from chickens flocks and hatcheries in Egypt.

“…The tested CoNS isolates susceptibility against 13 different available antimicrobial using disk diffusion method (Table14) [10,46]. Results of antibiotic susceptibility showed 100% resistance to Oxytetracycline 30 µg/ml, Trimethoprime + Sulphamethexole 2.25/23.75 µg/ml (SXT) , Calindamycin 2 µg/ml.…”

“…Coagulase-negative staphylococci (CoNS) , including many species such as S. hyicus [3], S. gallinarum [4], S.xylosus and S. epidermidis [5,6], and commonly been isolated from the nares and skin of healthy chickens, and their taxonomic positions were discussed apart from the pathogenicities. CoNS had been isolated from frozen and chilled industrialized, uncooked chicken parts or entire carcasses [7] , raw chicken's meat [8,9] , meat product [10] , cooked chicken products [11] , breast, neck and wing of chickens [12] , chicken carcasses herd-wise pooled neck skin samples [13] as well as poultry bioaerosol [14] .…”

Phytochemical screening and biological activities of Garcinia kola (bark, leaves and seeds) collected in Benin

“…The three fugal strains were part of the microorganisms isolated in a Beninese traditional cheese wagashi by Sessou et al (2012) Attien et al (2013) and stored in the Laboratory of Biology and Molecular Typing in Microbiology (University of Abomey-Calavi, Benin).…”