Prevalence and Activity of Epstein-Barr Virus and Human Cytomegalovirus in Symptomatic and Asymptomatic Apical Periodontitis Lesions

Hernádi, Katinka; Szalmás, Anita; Mogyorósi, Richard; Czompa, Levente; Veress, György; Csoma, Eszter; Márton, Ildikó; Kónya, József

doi:10.1016/j.joen.2010.06.008

Cited by 39 publications

(63 citation statements)

References 23 publications

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“…In elderly patients, the onset of primary oral lymphoma/LPD may be related to chronic inflammation that has been regarded as ''periodontitis''. In fact, EBV has been reportedly detected in apical and marginal periodontitis [21][22][23][24][25][26][27]. Most EBV-related oral tumors involve the tongue and the parotid gland, but some are located in the periodontium [1,7,8,10,11,20,27], as was the case in the present patient.…”

Section: Resultssupporting

confidence: 69%

A Case of Age-Related Epstein–Barr Virus (EBV)-Associated B Cell Lymphoproliferative Disorder, so-called Polymorphous Subtype, of the Mandible, with a Review of the Literature

Kikuchi

Fukunaga²,

Inoue

et al. 2012

Head and Neck Pathol

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Epstein-Barr virus (EBV) is known to be associated with the development of lymphomas in immunocompromised patients. Recently, age-related immune impairment has been recognized as a predisposing factor in the development of EBV-driven lymphoproliferative disorders (LPDs) in elderly patients without any known immunodeficiency or prior lymphoma. In approximately 70 % of reported cases, the affected sites have been extranodal, such as the skin, lung, tonsil and stomach. However, age-related EBV-associated B cell (EBV ? B cell) LPD is extremely rare in the oral cavity. Here we report a 71-year-old Japanese man who developed an EBV ? B cell LPD resembling classical Hodgkin lymphoma (CHL)-so-called polymorphous subtype-of the mandible. Histopathologically, infiltration of large atypical lymphoid cells including Hodgkin or Reed-Sternberg-like cells into granulation tissue with marked necrosis was found in the mandibular bone. Immunohistochemical analysis revealed that the large atypical Hodgkin or ReedSternberg-like cells were CD3-, CD15-, CD20?, CD30? and Epstein-Barr virus (EBV)-latent infection membrane protein-1 (LMP-1)?. In situ hybridization (ISH) demonstrated EBV-encoded small RNA (EBER) ? in numerous Hodgkin or Reed-Sternberg-like cells. EBNA-2 was detected by polymerase chain reaction (PCR) using an extract from the formalin-fixed, paraffin-embedded specimen. To our knowledge, this is the first reported case of the polymorphous subtype of age-related EBV ? B cell LPD affecting the mandible.

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Section: Resultssupporting

confidence: 69%

A Case of Age-Related Epstein–Barr Virus (EBV)-Associated B Cell Lymphoproliferative Disorder, so-called Polymorphous Subtype, of the Mandible, with a Review of the Literature

Kikuchi

Fukunaga²,

Inoue

et al. 2012

Head and Neck Pathol

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“…22 EBNA-2 mRNA is also associated with the symptoms of apical periodontitis. 23 The broad spectrum of EBNA-2 functions involves virus-host interactions, including cell signaling molecules, adapters, genes involved in cell cycle regulation, and leukocyte chemotaxins. 24 In light of the potential damage Epstein-Barr virus can cause in those living with HIV, we describe the results of screening for EBV and its subtypes, variables associated with EBV-HIV coinfection and EBNA-2 expression in an HIV-infected population.…”

Section: Introductionmentioning

confidence: 99%

Epstein-Barr virus in oral mucosa from human immunodeficiency virus positive patients

Santos

Azevedo

Silva

et al. 2014

Rev. Assoc. Med. Bras.

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Objective: the detection rate of Epstein-Barr virus (EBV) is higher in people living with human immunodeficiency virus (HIV). In an attempt to contribute to our epidemiological understanding of this coinfection and to investigate the activity of EBV in normal oral mucosa, we performed a cross-sectional study with HIV-positive patients. Methods: oral smears from 145 HIV-positive patients were collected between March 2010 and March 2011. Nested polymerase chain reaction (PCR) and reverse transcriptase-PCR (RT-PCR) were used to genotype EBV and to detect EBNA-2 expression, respectively. Results: EBV DNA was detected in 48.3% of the study participants, of whom 32.85% were EBV-1 and 45.71% were EBV-2 carriers. Additionally, 14.28% were coinfected with both types. EBNA-2 mRNA was expressed in 45.7% of the EBV--positive samples, including 20.0% with EBV-1 only, 20.0% with EBV-2 only and 1.4% with both genotypes. Immune status affected the overall EBV infection, and EBV-2 positivity was significantly correlated with sexual lifestyle of the participants. EBV co-infection with both viral types was dependent upon HIV viral load and the activity of the EBNA-2 gene. Conclusion: we report a high prevalence of active EBV in the oral mucosa of asymptomatic HIV-seropositive individuals. This study addresses the need for monitoring and treatment of HIV-infected patients with EBV reactivation.

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“…In another study, Ferreira et al [18] found EBV in 6% of purulent exudates from acute apical abscesses, whereas they did not identify HCMV in abscess samples using single and nested PCR. The discrepancies in the findings among our study and the three studies mentioned above might arise for several reasons, including differences in the primers used, methods used for DNA extraction, and host and environmental factors such as genetic background, ethnicity, and socioeconomic status [8,[20][21][22][23]. We used same sampling techniques applied by Chen et al [1].…”

Section: Discussionmentioning

confidence: 95%

Herpesviral and “Red Complex” Bacterial Analysis of Acute Apical Abscesses

Ozbek¹

2015

J Dent Probl Solut

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Introduction: To investigate the possible association between presence of Epstein-Barr virus, human cytomegalovirus and three endodontic bacterial pathogens (Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia) "red complex" in samples from patients with acute apical abscesses using real-time PCR.Methods: Periapical samples were collected from 18 acute apical abscess specimens and 6 control samples. The apical area of each study tooth was examined in periapical radiographs obtained using a long cone paralleling technique. The study included only single-rooted teeth that had carious lesions, necrotic pulps, and radiographic evidence of periradicular bone loss. No apparent communication from the abscess to the oral cavity or skin surface was observed. DNA extracts from purulent exudate aspirates of acute apical abscess and six control samples were evaluated for the presence of microbial and viral loads using real-time polymerase chain reaction (PCR) following the kit protocols recommended by the manufacturers. The chi squared test was used for statistical analysis.Results: HCMV, EBV, T. denticola, P. gingivalis, and T. forsythia were detected in 16.7 %, 5.6 %, 44.4 %, 27.7 %, and 22.2 % of the samples, respectively. As for the healthy pulps used as noninflamed controls, no control specimens contained bacterial or viral DNA. The pair HCMV/ T. denticola and HCMV/ T. forsythia was detected in one case. The association between HCMV, T denticola and T. forsythia was not statistically significant. Conclusion:Within the limitations of the present study, our PCR based findings revealed that in "red complex" bacteria, T. denticola and T. forsythia tended to occur in co-infection with HCMV.

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Prevalence and Activity of Epstein-Barr Virus and Human Cytomegalovirus in Symptomatic and Asymptomatic Apical Periodontitis Lesions

Cited by 39 publications

References 23 publications

A Case of Age-Related Epstein–Barr Virus (EBV)-Associated B Cell Lymphoproliferative Disorder, so-called Polymorphous Subtype, of the Mandible, with a Review of the Literature

A Case of Age-Related Epstein–Barr Virus (EBV)-Associated B Cell Lymphoproliferative Disorder, so-called Polymorphous Subtype, of the Mandible, with a Review of the Literature

Epstein-Barr virus in oral mucosa from human immunodeficiency virus positive patients

Herpesviral and “Red Complex” Bacterial Analysis of Acute Apical Abscesses

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