Pretreatment with β‐glucan attenuates isoprenaline‐induced myocardial injury in rats

Abstract

New Findings What is the central question of this study?The study was designed to assess whether pretreatment with β‐glucan could exert any protective action against isoprenaline‐induced myocardial injury in rats. What is the main finding and its importance?β‐Glucan pretreatment could reduce myocardial injury by restoring cardiac biomarkers, antioxidant status, apoptosis and histopathological changes. Therefore, β‐glucan might have the potential to be used in the prevention and/or treatment of myocardial infarction. Abstract This study was designed to investigate the cardioprotective effect of pretreatment with β‐glucan, the glucose polymer derived from the yeast Saccharomyces cerevisiae, against isoprenaline (ISO)‐induced myocardial injury in rats by studying biochemical cardiac markers, antioxidant parameters, apoptosis, ECG and histopathological changes. Male Sprague–Dawley rats were randomly divided into four treatment groups, namely control, β‐glucan, isoprenaline and β‐glucan + isoprenaline. The β‐glucan treatment group received β‐glucan (50 mg kg−1 day−1, p.o.) for 10 days. Myocardial injury was induced by ISO administration (100 mg kg−1, s.c.) twice, at an interval of 24 h, on the 9th and 10th days. Isoprenaline administration resulted in a marked increase in heart rate, ST segment elevation, myocardial malondialdehyde content, cardiac marker levels (lactate dehydrogenase, creatine kinase‐MB and high‐sensitivity cardiac troponin T) and apoptotic index, and a significant decrease in R‐wave amplitude and myocardial superoxide dismutase, catalase and glutathione peroxidase activities. In addition, apoptosis, congestion, necrosis, inflammatory cell infiltration and myofibrillar disorganization were observed histologically in myocardial tissue sections. The oral pretreatment with β‐glucan prevented almost all the parameters of isoprenaline‐induced myocardial injury in rats, and these findings were confirmed by the histopathological analysis. These findings provide evidence that β‐glucan could protect rat myocardium against ISO‐induced myocardial injury, and this was attributed to its antioxidant and anti‐apoptotic properties.

Effect of dietary supplementation with yeast Saccharomyces cerevisiae on skin, serum and liver of gilthead seabream (Sparus aurata L)

“…These observations are consistent with the detected reduction in the inflammation process demonstrated by light microscopy in skin from fish fed yeast. Similarly, improvements in oxidative defence (an increase in CAT, SOD and GR activities) were observed during acute oxidative stress in rodents receiving a supplement of β ‐glucan (derived from S. cerevisiae ) (Çetin, 2019) or in the liver antioxidant system of sea bass after S. cerevisiae supplementation (Santacroce et al ., 2012).…”

Effects of Peroxiredoxin 6 and Its Mutants on the Isoproterenol Induced Myocardial Injury in H9C2 Cells and Rats

“…ISO induction is a common method to construct a myocardial injury model. An excessive ISO will increase the oxygen consumption and contraction of myocardium to cause a myocardial injury [ 26 ]. In this study, the cell model and animal model of myocardial injury were established by the induction with ISO, and then the repair function of recombinant protein to the myocardial injury was studied.…”

Beta Glukanin Deneysel Böbrek İskemi Reperfüzyon Hasarina Karşi Koruyucu Etkileri

“…Clinical studies on beta glucan have also been increasing in recent years and it is being used in many supportive treatments Mavi Bulut A et al Beta Glucan and Renal Ischemia [17]. We investigated the possible effects of beta glucan administration as pre-treatment like various studies performed by other researchers [18,19]. Renal ischemia can be observed in clinical situations such as kidney transplantation, partial nephrectomy, sepsis, cardio-pulmonary bypass, various urological procedures and hydronephrosis.…”