Pretreatment with mineralocorticoid receptor blocker reduces intestinal injury induced by ischemia and reperfusion: involvement of inhibition of inflammatory response, oxidative stress, nuclear factor κB, and inducible nitric oxide synthase

Özaçmak, Hale Sayan; Özaçmak, Veysel Haktan; Barut, Figen; Araslı, Mehmet; Uçan, Bülent Hamdi

doi:10.1016/j.jss.2014.04.040

Cited by 30 publications

(28 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pretreatment with Sp provided a significant reduction in lipid peroxidation of the lung tissue, preventing the development of lung injury. In accordance with our findings, a considerable reduction in oxidative stress was also observed previous studies in renal [21] and intestinal I/R injury [26], both of which were treated with Sp. Moreover, it has been demonstrated that the administration of aldosterone induces ROS generation by activating the enzyme NADPH oxidase in cultured ventricular myocytes and mesangial cells in rats [21].…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Reduction of Acute Lung Injury by Administration of Spironolactone After Intestinal Ischemia and Reperfusion in Rats

Barut¹,

Özaçmak²,

TURAN³

et al. 2016

CIM

Self Cite

View full text Add to dashboard Cite

Purpose: Multiple organ failure, including acute lung injury, is a common complication of intestinal ischemia and reperfusion (I/R) injury and contributes to its high mortality rate. Activated polymorphonuclear neutrophils and reactive oxygen species contribute to the lung injury caused by intestinal I/R. Mineralokortikoid receptor antagonist spironolactone has a protective effect against I/R injury in animal models of retina, kidney, heart, and brain. The aim of the present study is to investigate the effect of aldosteron receptor blocker spironolactone on lung injury induced by intestinal I/R.Methods: Wistar albino rats were divided into four groups: (1) sham control; (2) intestinal I/R (30 min of ischemia by superior mesenteric artery occlusion followed by 3 h of reperfusion); (3) spironolactone pretreatment (20 mg/kg) + I/R; and, (4) spironolactone pretreatment without I/R. Spironolactone was given orally 3 days prior to intestinal I/R. A marker for lipid peroxidation (malondialdehyde; MDA), an indicator or oxidation state (reduced glutathione; GSH), an index of polymorphonuclear neutrophil sequestration (myeloperoxidase; MPO), inducible nitric oxide synthase (iNOS) immunoreactivity, and the histopathology of the lung tissue were analyzed.Results: Spironolactone pretreatment markedly reduced intestinal I/R-induced lung injury as indicated by histology and MDA and MPO levels. Moreover, the pretreatment decreased the iNOS immunoreactivity. Conclusion:The present study strongly suggests that spironolactone pretreatment decreased neutrophil infiltration, iNOS induction, oxidative stress, and histopathological injury in an experimental model of intestinal I/R induced-lung injury of rats.

show abstract

Section: Discussionsupporting

confidence: 93%

“…Several reports also indicate that Sp can reduce oxidative stress and inflammatory response [25]. Our previous study demonstrated that Sp, a aldosterone receptor antagonist, significantly decreases intestinal ischemic damage [26]; however, the effect of Sp on intestinal I/R-induced lung injury is unknown.…”

mentioning

confidence: 96%

Reduction of Acute Lung Injury by Administration of Spironolactone After Intestinal Ischemia and Reperfusion in Rats

Barut¹,

Özaçmak²,

TURAN³

et al. 2016

CIM

Self Cite

View full text Add to dashboard Cite

show abstract

“…The novelty of the present study is that the concentrations of iNOS and Caspase-3 were increased by oxidized fish oil but the increase was partially alleviated by supplementation of ISF. Caspase-3 also is activated by oxidative stress and helps to increase the inflammatory response (Ozacmak et al, 2014), which is consistent with our result. In addition, the concentration of NF-κB in this present study increased significantly in the young piglets fed oxidized fish oil diets, consistent with the hypothesis that oxidative stress in the intestinal mucosa increases ROS and then increases expression of proinflammatory cytokines via activating NF-κB (Aw, 1999).…”

Section: Discussionsupporting

confidence: 93%

Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil

Huang

Jiang

et al. 2016

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:To investigate the effect of glycitein, a synthetic soybean isoflavone (ISF), on the intestinal antioxidant capacity, morphology, and cytokine content in young piglets fed oxidized fish oil, 72 4-d-old male piglets were assigned to three treatments. The control group was fed a basal diet containing fresh fish oil, and the other two groups received the same diet except for the substitution with the same dosage of oxidized fish oil alone or with ISF (oxidized fish oil plus ISF). After 21 d of feeding, supplementation of oxidized fish oil increased the levels of malondialdehyde (MDA), oxidized glutathione (GSSG), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), nuclear factor κ B (NF-κB), inducible nitric oxide synthase (iNOS), NO, and Caspase-3 in jejunal mucosa, and decreased the villous height in duodenum and the levels of secretory immunoglobulin A (sIgA) and IL-4 in the jejunal mucosa compared with supplementation with fresh oil. The addition of oxidized fish oil plus ISF partially alleviated this negative effect. The addition of oxidized fish oil plus ISF increased the villous height and levels of sIgA and IL-4 in jejunal mucosa, but decreased the levels of IL-1β and IL-2 in jejunal mucosa (P<0.05) compared with oxidized fish oil. Collectively, these results show that dietary supplementation of ISF could partly alleviate the negative effect of oxidized fish oil by improving the intestinal morphology as well as the antioxidant capacity and immune function in young piglets.

show abstract

“…Activation of the MR in the vasculature and inflammatory cells has a large impact on kidney disease development and associated comorbidities by promoting increased oxidative injury, vasoactive imbalance, endothelium activation, vascular calcification and increased pro-inflammatory infiltrate (Figure 1). These effects are not restricted to the kidney but may also affect other organs, such as the heart, 34,38,85 intestine, 86 brain, 37,87,88 vessels, 26,27,89 eye 90,91 and skin. 92,93 (For F I G U R E 1 Pathological mechanisms of MR activation in endothelial cells, smooth muscle cells, T cells and macrophages that promote kidney injury and contribute to various forms of kidney disease a review see 94 ).…”

Section: Discussionmentioning

confidence: 99%

Vascular and inflammatory mineralocorticoid receptors in kidney disease

Barrera‐Chimal

Jaisser

2019

Acta Physiologica

View full text Add to dashboard Cite

Mineralocorticoid receptor (MR) activation in the kidney can occur outside the aldosterone-sensitive distal nephron in sites including the endothelium, smooth muscle and inflammatory cells. MR activation in these cells has deleterious effects on kidney structure and function by promoting oxidative injury, endothelial dysfunction and stiffness, vascular remodelling and calcification, decreased relaxation and activation of T cells and pro-inflammatory macrophages. Here, we review the data showing the cellular consequences of MR activation in endothelial, smooth muscle and inflammatory cells and how this affects the kidney in pathological situations. The evidence demonstrating a benefit of pharmacological or genetic MR inhibition in various models of kidney disease is also discussed.

show abstract

Pretreatment with mineralocorticoid receptor blocker reduces intestinal injury induced by ischemia and reperfusion: involvement of inhibition of inflammatory response, oxidative stress, nuclear factor κB, and inducible nitric oxide synthase

Cited by 30 publications

References 51 publications

Reduction of Acute Lung Injury by Administration of Spironolactone After Intestinal Ischemia and Reperfusion in Rats

Reduction of Acute Lung Injury by Administration of Spironolactone After Intestinal Ischemia and Reperfusion in Rats

Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil

Vascular and inflammatory mineralocorticoid receptors in kidney disease

Contact Info

Product

Resources

About