Effects of soybean isoflavone on intestinal antioxidant capacity and cytokines in young piglets fed oxidized fish oil

Abstract

Abstract:To investigate the effect of glycitein, a synthetic soybean isoflavone (ISF), on the intestinal antioxidant capacity, morphology, and cytokine content in young piglets fed oxidized fish oil, 72 4-d-old male piglets were assigned to three treatments. The control group was fed a basal diet containing fresh fish oil, and the other two groups received the same diet except for the substitution with the same dosage of oxidized fish oil alone or with ISF (oxidized fish oil plus ISF). After 21 d of feeding, supplementation of oxidized fish oil increased the levels of malondialdehyde (MDA), oxidized glutathione (GSSG), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), nuclear factor κ B (NF-κB), inducible nitric oxide synthase (iNOS), NO, and Caspase-3 in jejunal mucosa, and decreased the villous height in duodenum and the levels of secretory immunoglobulin A (sIgA) and IL-4 in the jejunal mucosa compared with supplementation with fresh oil. The addition of oxidized fish oil plus ISF partially alleviated this negative effect. The addition of oxidized fish oil plus ISF increased the villous height and levels of sIgA and IL-4 in jejunal mucosa, but decreased the levels of IL-1β and IL-2 in jejunal mucosa (P<0.05) compared with oxidized fish oil. Collectively, these results show that dietary supplementation of ISF could partly alleviate the negative effect of oxidized fish oil by improving the intestinal morphology as well as the antioxidant capacity and immune function in young piglets.

Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs

“…In addition to morphology measurements, jejunal antioxidant capacity and tight junction were also analysed to explore intestinal health of growing pigs . Under oxidative stress conditions, reactive oxygen species synthesis can trigger greater prevalence of pathogenic microorganisms as a result of compromised immune cell functions, which could subsequently lead to defects in gastrointestinal barrier function .…”

“…In addition to morphology measurements, jejunal antioxidant capacity and tight junction were also analysed to explore intestinal health of growing pigs. 18,39,40 Under oxidative stress conditions, reactive oxygen species synthesis can trigger greater prevalence of pathogenic microorganisms as a result of compromised immune cell functions, which could subsequently lead to defects in gastrointestinal barrier function. 41,42 The present study showed that there were no differences in activity of antioxidant enzyme and protein expression of occludin, claudin1 and claudin3 among pigs consuming colistin sulfate or lysozyme diets.…”

Duration of the flaxseed diet promotes deposition of n-3 fatty acids in the meat and skin of Peking ducks

“…The homogenates were centrifuged (4,000 g for 5 min at 4°C) and the supernatants were used to determine the indices of oxidative stress. The oxidative indicators, SOD and MDA, in the breast muscle, liver, and jejunal mucosa were quantified using assay kits (Nanjing Jiancheng Bioengineering Institute, China) following the manufacturer’s instructions (31).…”

Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus

“…Huang et al [19] reported that SI improved the immune function in young piglets fed oxidized fish oil. Moreover, Lv et al [42] reported that genistein (GEN boosted the anti-viral capacity of broilers chickens.…”

“…Isoflavones, including genistein, daidzein, and glycitein, are similar in structure to 17-β-estradiol. Soy isoflavones are used as a supplement to improve growth performance, antioxidant activity, and immune function [14,15,16,17,18,19]. These reasons caused us to hypothesize that supplemental soy isoflavones may improve the performance and immune function of broilers chickens infected with IBDV.…”