Pretreatment with Interleukin-1 Enhances Survival of Sympathetic Ganglionic Neuron Grafts

Nakao, Naoyuki; Itakura, Toru; Uematsu, Yoshihiko; Nakai, Mitsukazu; Komai, Norihiko; Furukawa, Shoei

doi:10.2176/nmc.34.407

Cited by 16 publications

(10 citation statements)

References 16 publications

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“…Other cytokines used by the immune system, such as interleukin (IL)‐1 and IL‐11, have previously been shown to play a role in the conversion of mesencephalic‐derived progenitors to DA neurons [17]. IL‐1 also exerts trophic effects on DA and other catecholaminergic neurons both in vitro and in vivo [27, 28]. Thus, combined, emerging evidence indicates that signaling molecules typically used postnatally by the immune system, such as interleukins and chemokines, play an essential role during embryonic development of the CNS.…”

Section: Discussionmentioning

confidence: 99%

α-Chemokines Regulate Proliferation, Neurogenesis, and Dopaminergic Differentiation of Ventral Midbrain Precursors and Neurospheres

et al. 2008

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Increasing evidence suggests that ␣-chemokines serve several important functions in the nervous system, including regulation of neuroimmune responses, neurotransmission, neuronal survival, and central nervous system development. In this study, we first examined the function of two ␣-chemokines, chemokine ligand (CXCL) 6 and CXCL8, and their receptors, CXCR1 and CXCR2, in the developing rat ventral midbrain (VM). We found that CXCR2 and CXCL6 are regulated during VM development and that CXCL6 promotes the differentiation of nurr77-related receptor (Nurr1)؉ precursors into dopaminergic (DA) neurons in vitro. Intriguingly, CXCL8, a ligand expressed only in Homo sapiens, enhanced progenitor cell division, neurogenesis, and tyrosine hydroxylasepositive (TH؉) cell number in rodent precursor and neurosphere cultures. CXCL1, the murine ortholog of CXCL8, was developmentally regulated in the VM and exhibited activities similar but not identical to those of CXCL8. TH؉ cells derived from chemokine-treated VM neurospheres coexpressed Nurr1 and VMAT and were functionally active, as shown by calcium (Ca 2؉ ) fluxes in response to AMPA. In conclusion, our data demonstrate that CXCL1, CXCL6, and CXCL8 increase the number of DA neurons in VM precursor and neurosphere cultures by diverse mechanisms. Thus, ␣-chemokines may find an application in the preparation of cells for drug development or Parkinson's disease cell replacement therapy.

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Section: Discussionmentioning

confidence: 99%

α-Chemokines Regulate Proliferation, Neurogenesis, and Dopaminergic Differentiation of Ventral Midbrain Precursors and Neurospheres

et al. 2008

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“…It has been reported that IL-1␤ supports survival of fetal rat dopaminergic neurons (Akaneya et al, 1995) and of neonatal rat superior cervical ganglion explants (Nakao et al, 1994). Because the latter effect could be blocked by application of aNGF, it was probably mediated by nonneuronal ganglionic cells.…”

Section: Co-cultures Influenced By the Cytokines Il-1␤ Il-6 Or Lifmentioning

confidence: 94%

“…To achieve that purpose we used co-cultures of DRG neurons and sFLCs or pFLCs. Most previous studies on cytokine effects on neuron survival or neuritogenesis in vitro have used purely neuronal cultures (Akaneya et al, 1995;Kurek et al, 1996;Barrett et al, 1998;März et al, 1998;Thier et al, 1999) or nerve tissue explants (Shadiack et al, 1993;Nakao et al, 1994;Ludlam et al, 1995;Horie et al, 1997;Ekström et al, 2000). Chalazonitis et al (1992) studied cytokine effects on neurons in a co-culture system similar to the one used here and the effects of LIF have been studied in mast cell/fibroblast co-cultures (Hiragun et al, 2000).…”

Section: Co-cultures Influenced By the Cytokines Il-1␤ Il-6 Or Lifmentioning

confidence: 99%

Effects of IL‐1β, IL‐6 or LIF on rat sensory neurons co‐cultured with fibroblast‐like cells

Edoff

Jerregård

2001

J of Neuroscience Research

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Inflammation may affect the local presence of sensory nerve fibers in situ and inflammatory mediators influence sensory neurons in vitro. In the present study we have investigated effects of the cytokines interleukin-1beta (IL-1beta, interleukin-6 (IL-6), and leukemia inhibitory factor (LIF) on survival of and neurite growth from neonatal rat sensory neurons co-cultured with fibroblast-like cells prepared from neonatal rat skin (sFLCs) or perichondrium (pFLCs). The results showed that both FLC types expressed receptors for all three cytokines. Five ng/ml of either cytokine, but not lower or higher concentrations, supported survival of DRG neurons co-cultured with sFLCs. Neuronal survival was also enhanced by addition of the soluble IL-6 receptor (rsIL-6R) with or without IL-6. In co-cultures with pFLCs neuronal survival was promoted by IL-6, increasing with cytokine concentration. Addition of rsIL-6R without IL-6 did also stimulate neuronal survival. The growth of neurites from DRG neurons co-cultured with sFLCs was stimulated by 0.5 ng/ml LIF, unaffected by 5 ng/ml LIF and inhibited by 50 ng/ml LIF. Considering DRG neurons co-cultured with pFLCs, 50 ng/ml of either of the three cytokines, as well as rsIL-6R conditioned medium, stimulated neurite outgrowth. Some of the cytokine effects observed were reduced by application of antibodies against nerve growth factor (NGF). We conclude that that the cytokines examined affect DRG neurons in terms of survival or neuritogenesis, that the effects are influenced by cytokine concentration and the origin of the FLCs and that some of the effects are indirect, probably being mediated by factors released from FLCs.

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“…The trophic effects of IL-1 on DA and other catecholaminergic neurons also have been documented both in in vitro and in vivo experiments (Akaneta et al 1995;Nakao et al 1994). Thus, similar to their effects on hematopoietic cell development, cytokines such as IL-1 likely play a role in CNS development and differentiation.…”

Section: Discussionmentioning

confidence: 92%

Cytokine Induction of Dopamine Neurons from Progenitor Cells

Carvey¹

2000

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Pretreatment with Interleukin-1 Enhances Survival of Sympathetic Ganglionic Neuron Grafts

Cited by 16 publications

References 16 publications

α-Chemokines Regulate Proliferation, Neurogenesis, and Dopaminergic Differentiation of Ventral Midbrain Precursors and Neurospheres

α-Chemokines Regulate Proliferation, Neurogenesis, and Dopaminergic Differentiation of Ventral Midbrain Precursors and Neurospheres

Effects of IL‐1β, IL‐6 or LIF on rat sensory neurons co‐cultured with fibroblast‐like cells

Cytokine Induction of Dopamine Neurons from Progenitor Cells

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