“…There is a growing interest in the development of in vitro models that can accurately simulate the natural regeneration process of neurons following a PNI. Monolayer in vitro systems have been developed by co‐culturing neurons with Schwann cells to study the influence of cell‐to‐cell interactions (Clarke et al, ), however, more complex 3D co‐culture systems have been developed to mimic the natural nerve environment more closely such as; explant cultures (Yip et al, ; Miller et al, ; Vikman et al, ; Lesuisse and Martin, ) self‐assembled aggregate cultures (Knoops et al, ; Becq et al, ; Jerregard, ; Lillesaar et al, ; Ruscheweyh and Sandkuhler, ; Edoff and Jerregard, ; Lee et al, ), and scaffold based cell cultures (Borkenhagen et al, ; Dubey et al, ; Backstrom et al, ; Balgude et al, ; Yu and Bellamkonda, ; Georgiou et al, ).…”