Pretreatment and saccharification of rice hulls for the production of fermentable sugars

Wei, Gongyuan; Gao, Wa; Jin, II-Hyuck; Yoo, Seung-Yeop; Lee, Jai‐Heon; Chung, Chong-Pyoung; Lee, Jin Woo

doi:10.1007/s12257-009-0029-8

Cited by 52 publications

(26 citation statements)

References 21 publications

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“…Ang et al (2012) and Wei et al (2009) suggest that pretreatment is a crucial step to improve the efficiency of rice husk bioconversion and to obtain a high yield of fermentable sugars through enzymatic hydrolysis. Although various pretreatment techniques have been developed, such as acid, alkali, organosolvent, and ionic liquids, the high cost of chemicals and their environmental toxicity are major impediments to chemical utilization in biofuel production.…”

Section: Introductionmentioning

confidence: 99%

Influence of High-Pressure Steam Pretreatment on the Structure of Rice Husk and Enzymatic Saccharification in a Two-Step System

et al. 2017

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This study aimed at developing an operational high-pressure steam pretreatment (HPSP) to effectively modify rice husk for enzymatic saccharification. The HPSP was performed at 160 to 200 °C under 0.3 to 2.8 MPa for 2 to 10 min. The efficiency of this method was based on the chemical composition, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and X-ray diffraction (XRD) analyses. Optimum pretreatment conditions (200 °C, 1.85 MPa for 7 min), enzyme concentration at 30 FPU/g and temperature at 60 °C for 48 h of continuous saccharification effectively produced sugar (21.1 g/L = 0.422 g/g dry substrate) at a saccharification degree of 53.87%. Conducting a secondstep enzymatic saccharification resulted in additional sugar production (7.9 g/L = 0.158 g/g substrate) and a 20.44% saccharification degree. In contrast, the two-step saccharification process (48 and 24 h) achieved optimal sugar yield of 0.581 g/g substrate and saccharification degree of 73.5%. Additionally, the process improved the yield of monomeric sugars of glucose (0.465 g/g), xylose (0.010 g/g), and cellobiose (0.063 g/g). Therefore, the combination of the high-pressure steam pretreatment with thermostable cellulase from Bacillus licheniformis 2D55 in a two-step enzymatic saccharification process is an economically viable method in rice husk bioprocessing for sugar production.

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Section: Introductionmentioning

confidence: 99%

Influence of High-Pressure Steam Pretreatment on the Structure of Rice Husk and Enzymatic Saccharification in a Two-Step System

et al. 2017

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“…Rice hulls, as lignocellulosic materials, have been hydrolyzed for production of fermentable sugars by commercial cellulases, in which the major cellulase was carboxymethylcellulase (CMCase) (Wei et al 2009(Wei et al , 2010. In this study, cellulases were used for hydrolysis of the dried fruits of A. grossedentata to develop a safe and economical process for the enhanced recovery yield of ampelopsin .…”

Section: Introductionmentioning

confidence: 99%

Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata

Gao¹,

Lee²,

Li³

et al. 2016

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The commercial method for isolation of ampelopsin, one of the most common flavonoids isolated from the plant species Ampelopsis grossedentata, is a simple hydrothermal extraction at high temperature. To develop an improved process to isolate ampelopsin, the effects of treatment of cellulase on hydrolysis of the dried fruit of A. grossedentata were investigated. The treatment of cellulase was found to decrease the temperature and time for hydrolysis of the dried fruit of A. grossedentata. The conditions of the filter press and continuous flow centrifuge for removal of insoluble materials from the hydrolysate of the dried fruit of A. grossedentata were optimized. The recovery yield of ampelopsin from the dried fruits of A. grossedentata was 39.4%, as determined by HPLC chromatographic analysis. A safe and economical process at low temperature with treatment of cellulase for the isolation of ampelopsin was developed in this study.

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“…The enzymatic saccharification of agricultural by-products for the production of ethanol uses commercial cellulases. Because such enzymes also have the ability to degrade carboxymethylcellulose (CMC), they are sometimes evaluated by their ability to decompose CMC, and they have been called carboxymethylcellulase (CMCase) by some authors (Wei et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Enhanced Production of Cellulase from the Agricultural By-product Rice bran by Escherichia coli JM109/LBH-10 with a Shift in Vessel Pressure of a Pilot-scale Bioreactor

Jong

Lee

2016

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The optimal vessel pressure of the bioreactor for cell growth and the production of cellulase, as well as the effect of a shift in pressure within the reactor on cellulase production were investigated. The optimal vessel pressure for the cell growth of E. coli JM109/LBH-10 was 0.08 MPa, whereas that for the production of cellulase was 0.04 MPa. The maximal production of cellulase by E. coli JM109/LBH-10 with a shift in the vessel pressure from 0.08 to 0.04 MPa after 24 h was 636.8 U/mL, which was 1.2 times higher than that without a shift. The shift in vessel pressure optimized for cell growth to that for the production of cellulase after the mid-term log-phase resulted in higher cell growth and cellulase production. A simple process with a shift in the vessel pressure of bioreactors to enhance the production of cellulase from agricultural by-products has been developed and can be directly applied to the industrial-scale production of cellulases.

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Pretreatment and saccharification of rice hulls for the production of fermentable sugars

Cited by 52 publications

References 21 publications

Influence of High-Pressure Steam Pretreatment on the Structure of Rice Husk and Enzymatic Saccharification in a Two-Step System

Influence of High-Pressure Steam Pretreatment on the Structure of Rice Husk and Enzymatic Saccharification in a Two-Step System

Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata

Enhanced Production of Cellulase from the Agricultural By-product Rice bran by Escherichia coli JM109/LBH-10 with a Shift in Vessel Pressure of a Pilot-scale Bioreactor

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