Pretreatment and fractionation of lignocellulosic biomass for production of biofuel and value-added products

Yoo, Chang Geun

doi:10.31274/etd-180810-2355

Cited by 4 publications

(8 citation statements)

References 95 publications

(86 reference statements)

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“…Endo-cellulases attack the β-1,4-glycosidic bonds randomly, targeting the amorphous region along the cellulose structure and produce either cellobiose or glucose. On the other hand, exo-cellulases act on the linkages from the non-reducing end of cellulose releasing cellobiose as the main reaction product [36]. Similar patterns were observed for the hemicellulose-derived monosaccharides in this study.…”

Section: Enzymatic Hydrolysis Of Non-treated and Thermally Pre-treatesupporting

confidence: 84%

“…According to the manufacturer's datasheet (Sigma Aldrich), cellulase contained mainly endo-and exo-cellulases, thus their documented synergistic activities led to the effective hydrolysis of cellulose and β-glucans [35,36]. Endo-cellulases attack the β-1,4-glycosidic bonds randomly, targeting the amorphous region along the cellulose structure and produce either cellobiose or glucose.…”

Section: Enzymatic Hydrolysis Of Non-treated and Thermally Pre-treatementioning

confidence: 99%

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Rapeseed meal hydrolysate as substrate for microbial astaxanthin production

Harith

Charalampopoulos

Chatzifragkou

2019

Biochemical Engineering Journal

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Rapeseed meal, a by-product of oil processing industry, was evaluated as a substrate for astaxanthin production by the yeast Xanthophyllomyces dendrorhous DSMZ 5626. Four commercial enzymes were tested at different concentrations (1-15 %, v/v) for their ability to break down the cellulosic and hemicellulosic compounds of rapeseed meal into fermentable sugars. Viscozyme® L and cellulase demonstrated the highest glucose recovery yields (47-52%, w/w for 15 % (v/v) of enzyme loading) with 7-11 g/l of net glucose released in the hydrolysates. Pectinase and Accellerase® hydrolysates supported the best cell growth and astaxanthin production in batch shake flask cultures, with maximum biomass of 26 g/l and 15 g/l, respectively, and astaxanthin yields (YP/X) of 258 to 332 µg per g of biomass. In batch bioreactor trials, pectinase hydrolysates resulted in high biomass (42 g/l) and astaxanthin production (11 mg/l) aided by the presence of glycerol (originating from the enzyme formulation) which served as additional energy and carbon source. Finally, simple glass beads disruption lead into satisfactory astaxanthin extraction (95 %, w/w) in acetone. The findings of this study generate knowledge towards scale-up potential of microbial astaxanthin production using rapeseed meal hydrolysate as fermentation feedstock.

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Section: Enzymatic Hydrolysis Of Non-treated and Thermally Pre-treatesupporting

confidence: 84%

Section: Enzymatic Hydrolysis Of Non-treated and Thermally Pre-treatementioning

confidence: 99%

Rapeseed meal hydrolysate as substrate for microbial astaxanthin production

Harith

Charalampopoulos

Chatzifragkou

2019

Biochemical Engineering Journal

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“…The ultra‐dissolution and release of hemicellulose in ZnCl 2 .4H 2 O (>95% of total hemicellulosic content ) (Table ) was a profound observation. Yoo et al reported up to 96% solvation of hemicellulosic content in corn stover dissolved in ZnCl 2 .4H 2 O, relating the high yield to the efficiency of the solvent's ability to fractionate biomass …”

Section: Resultsmentioning

confidence: 99%

Effect of water of crystallization on the dissolution efficiency of molten zinc chloride hydrate salts during the pre‐treatment of corncob biomass

Awosusi

Adeleke

Ayeni

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND: Agro-wastes albeit abundant and cheap, require pre-treatment steps tailored to overcome the characteristic recalcitrance of lignocellulosic biomass. In this study, zinc chloride hydrate salts were studied for their biomass deconstruction potential. The role of salts' hydration and to what degree it affects the dissolution efficiency was evaluated. Milled corn cobs from local farmlands were dissolved in the following solvent systems; ZnCl 2 .3H 2 O, ZnCl 2 .4H 2 O, ZnCl 2 .5H 2 O and ZnCl 2 .7H 2 O at 70 ∘ C for 60 mins at a biomass to solvent ratio of 1:12. RESULTS: The difference between the least and most hydrated solvent systems in terms of total released sugars as a percentageof recoverable sugar is 70.1%. The ZnCl 2 .4H 2 O solvent system extracted up to 49.1% of glucose and 95% of xylose. There were significant differences in the crystallinity indices and surface morphologies between the untreated and treated biomass. CONCLUSION: The findings as documented contribute to current insights on the mechanisms of disintegration of lignocellulosic biomass in molten hydrate salts. The extraction of sugar components of biomass by ZnCl 2 .4H 2 O solvation was about 43% glucan and 99% xylan.

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“…Komponen terbesar penyusun TKKS terdiri atas 45,95% selulosa, 16,49% hemiselulosa dan 22,48% lignin (Wardani, 2014). Menurut Yoo (2012) dan North (2014) setiap komponen tersebut dapat diubah menjadi produk yang lebih bernilai guna dan bernilai jual. Selulosa yang merupakan komponen terbesar, dapat diolah menjadi sumber energi/bahan bakar cair berupa bioetanol, asam organik, pelarut, pelumas dan polimer.…”

Section: Pendahuluanunclassified

“…Hemiselulosa mengandung xilan yang terdiri atas unit-unit xilosa, dapat diolah menjadi etanol, xilitol (pemanis buatan), resin, dan nylon. Sementara itu, lignin dapat diolah menjadi bahan dasar kimia organik, batu bara dan bahan bakar padat (North, 2014;Yoo, 2012).…”

Section: Pendahuluanunclassified

Proses Fraksionasi Biomassa dari Tandan Kosong Kelapa Sawit melalui Metode Organosolv Etanol dengan Penambahan Katalis

2020

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Tandan kosong kelapa sawit (TKKS) merupakan salah satu biomassa lignoselulosa dengan komposisi utama terdiri atas selulosa, hemiselulosa, dan lignin. Pemisahan komponen lignoselulosa dapat dilakukan dengan fraksionasi melalui metode organosolv. Penelitian ini bertujuan untuk mendapatkan kondisi operasi dari proses fraksionasi biomassa lignoselulosa TKKS dengan metode organosolv berkatalis NH4OH. Penelitian dirancang menggunakan Metode Placket-Burman dengan variasi rasio fraksi berat, rasio etanol:air, temperatur, konsentrasi katalis, dan waktu. Hasil penelitian menunjukkan bahwa fraksionasi dapat dilakukan dengan baik pada rasio fraksi berat biomassa 10%, perbandingan etanol:air sebanyak 1:1, temperatur 160°C, dan konsentrasi katalis 5% NH4OH, selama 120 menit. Kondisi tersebut dapat menghasilkan perolehan selulosa 98,07%, perolehan hemiselulosa 98,71% dan penghilangan lignin 60,28%. Penggunaan kembali pelarut dilakukan dengan memisahkan lignin melalui presipitasi H2SO4 1M pada pH 2. Penggunaan pelarut untuk kedua kali dapat menghasilkan perolehan selulosa 98,03%, perolehan hemiselulosa 65,61% dan penghilangan lignin 59,81%. Sementara itu, dari perhitungan neraca massa, dapat dilihat bahwa proses tersebut dapat dilakukan karena kehilangan pelarut dan padatan relatif sedikit. Kehilangan padatan berkisar pada 19,06% dari umpan TKKS, sedangkan pelarut yang dapat diambil adalah sekitar 59,34% dari umpan etanol.

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Pretreatment and fractionation of lignocellulosic biomass for production of biofuel and value-added products

Abstract: Introduction Materials and Methods Results and Discussion Conclusions Acknowledgements

Cited by 4 publications

References 95 publications

Rapeseed meal hydrolysate as substrate for microbial astaxanthin production

Rapeseed meal hydrolysate as substrate for microbial astaxanthin production

Effect of water of crystallization on the dissolution efficiency of molten zinc chloride hydrate salts during the pre‐treatment of corncob biomass

Proses Fraksionasi Biomassa dari Tandan Kosong Kelapa Sawit melalui Metode Organosolv Etanol dengan Penambahan Katalis

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