Preterm birth: Born too soon for the developing airway epithelium?

Looi, Kevin; Evans, Denby J.; Garratt, Luke W.; Ang, Sherlynn; Hillas, Jessica; Kicic, Anthony; Simpson, Shannon J.

doi:10.1016/j.prrv.2018.11.003

Cited by 11 publications

(8 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In neonatal settings prematurity represents the single most important factor influencing mortality and long-term morbidity, including lung conditions associated with aberrant developmental programs 10 , 17 – 19 . The understanding of these programs has been hindered by the paucity of systems to properly assess the intrinsic properties of the epithelial barrier and their response to the extra-uterine environment.…”

Section: Resultsmentioning

confidence: 99%

Prematurity alters the progenitor cell program of the upper respiratory tract of neonates

Shui

Wang

Liu

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The impact of prematurity on human development and neonatal diseases, such as bronchopulmonary dysplasia, has been widely reported. However, little is known about the effects of prematurity on the programs of stem cell self-renewal and differentiation of the upper respiratory epithelium, which is key for adaptation to neonatal life. We developed a minimally invasive methodology for isolation of neonatal basal cells from nasopharyngeal (NP) aspirates and performed functional analysis in organotypic cultures to address this issue. We show that preterm NP progenitors have a markedly distinct molecular signature of abnormal proliferation and mitochondria quality control compared to term progenitors. Preterm progenitors had lower oxygen consumption at baseline and were unable to ramp up consumption to the levels of term cells when challenged. Although they formed a mucociliary epithelium, ciliary function tended to decline in premature cells as they differentiated, compared to term cells. Together, these differences suggested increased sensitivity of preterm progenitors to environmental stressors under non-homeostatic conditions.

show abstract

Section: Resultsmentioning

confidence: 99%

Prematurity alters the progenitor cell program of the upper respiratory tract of neonates

Shui

Wang

Liu

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Intra-amniotic infections (IAI) are one of the reasons for the preterm birth of vulnerable infants [2]. Alleviation of IAI impact can increase the length of gestation and postpone the preterm birth (PTB), which beneficially supports organ development, mainly of the lungs [36]. The possibility of the experimental work in the preterm infant is very limited, e.g., to a passive collection of various data, and experiments with other primates have ethical and financial limitations.…”

Section: Discussionmentioning

confidence: 99%

High Mobility Group Box 1 in Pig Amniotic Membrane Experimentally Infected with E. coli O55

Šplı́chal

Šplı́chalová

2021

Biomolecules

View full text Add to dashboard Cite

Intra-amniotic infections (IAI) are one of the reasons for preterm birth. High mobility group box 1 (HMGB1) is a nuclear protein with various physiological functions, including tissue healing. Its excessive extracellular release potentiates inflammatory reaction and can revert its action from beneficial to detrimental. We infected the amniotic fluid of a pig on the 80th day of gestation with 1 × 104 colony forming units (CFUs) of E. coli O55 for 10 h, and evaluated the appearance of HMGB1, receptor for glycation endproducts (RAGE), and Toll-like receptor (TLR) 4 in the amniotic membrane and fluid. Sham-infected amniotic fluid served as a control. The expression and release of HMGB1 were evaluated by Real-Time PCR, immunofluorescence, immunohistochemistry, and ELISA. The infection downregulated HMGB1 mRNA expression in the amniotic membrane, changed the distribution of HMGB1 protein in the amniotic membrane, and increased its level in amniotic fluid. All RAGE mRNA, protein expression in the amniotic membrane, and soluble RAGE level in the amniotic fluid were downregulated. TLR4 mRNA and protein expression and soluble TLR4 were all upregulated. HMGB1 is a potential target for therapy to suppress the exaggerated inflammatory response. This controlled expression and release can, in some cases, prevent the preterm birth of vulnerable infants. Studies on suitable animal models can contribute to the development of appropriate therapy.

show abstract

“…Current evidence suggests that the airway epithelium is both structurally and functionally abnormal after preterm birth, with reports of epithelial thickening and goblet cell hyperplasia in addition to increased inflammation and apoptosis in the neonatal intensive care unit (NICU). Preterm lungs also have reduced amounts of collagen and elastin, which, associated with abnormalities in surfactant, cause a reduced functional residual capacity 31 …”

Section: Methodsmentioning

confidence: 99%

Pulmonary pneumatoceles in neonates

Rocha

2020

Pediatric Pulmonology

View full text Add to dashboard Cite

Pulmonary pneumatoceles were relatively common in neonates in the pre‐surfactant era. In the current era of surfactant, noninvasive and gentle invasive ventilation there is a paucity of data on clinical characteristics and outcomes of pneumatoceles in neonates. The lesion generally resolves spontaneously, but a few cases follow a complicated course with formation of extensive and expanding lesions. To better understand the pathophysiology, clinical significance, natural history, complications, treatment options and prognosis of pulmonary pneumatoceles in neonates, an extensive research was performed on the databases of medical literature. The information collected in this review is important for the clinicians in decision‐making, especially in the most difficult cases.

show abstract

Preterm birth: Born too soon for the developing airway epithelium?

Cited by 11 publications

References 87 publications

Prematurity alters the progenitor cell program of the upper respiratory tract of neonates

Prematurity alters the progenitor cell program of the upper respiratory tract of neonates

High Mobility Group Box 1 in Pig Amniotic Membrane Experimentally Infected with E. coli O55

Pulmonary pneumatoceles in neonates

Contact Info

Product

Resources

About