High Mobility Group Box 1 in Pig Amniotic Membrane Experimentally Infected with E. coli O55

Šplı́chal, Igor; Šplı́chalová, Alla

doi:10.3390/biom11081146

Cited by 4 publications

(2 citation statements)

References 83 publications

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“…DAMPs and PAMPs bind to pattern recognition receptors on immune cells and tissue to induce an inflammatory immune response. Their DAMP of interest was the High Mobility Group Box 1 (HMGB1) nuclear protein [121]. Amniotic membrane expression of HMGB1 was downregulated during intra-amniotic infection, whereas its release in amniotic fluid was increased.…”

Section: Targeting Indicators Of Maternal Immune Responsementioning

confidence: 99%

Does Precision-Based Medicine Hold the Promise of a New Approach to Predicting and Treating Spontaneous Preterm Birth?

Khan,

Singh,

Yovera Leyva

et al. 2024

IJTM

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Background: Preterm birth (PTB) is a leading cause of childhood disability, and it has become a key public health priority recognized by the World Health Organization and the United Nations. Objectives: This review will: (1) summarize current practice in the diagnosis and management of PTB, (2) outline developments in precision-based medicine for diagnostics to improve the care provided to pregnant women at risk of PTB, and (3) discuss the implications of current research in personalized medicine and the potential of future advances to influence the clinical care of women at risk of PTB. Methodology: This is a narrative literature review. Relevant journal articles were identified following searches of computerized databases. Key Results: Current and emerging technologies for the utility of personalized medicine in the context of PTB have the potential for applications in: (1) direct diagnostics to identify and target infection as one of the main known causes of PTB, (2) identifying novel maternal and fetal biomarkers, (3) the use of artificial intelligence and computational modeling, and (4) combining methods to enhance diagnosis and treatment. Conclusions: In this paper, we show how current research has moved in the direction of the targeted use of biomarkers in the context of PTB, with many novel approaches.

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Section: Targeting Indicators Of Maternal Immune Responsementioning

confidence: 99%

Does Precision-Based Medicine Hold the Promise of a New Approach to Predicting and Treating Spontaneous Preterm Birth?

Khan,

Singh,

Yovera Leyva

et al. 2024

IJTM

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show abstract

“…Currently, animal models for studying IAI mainly consist of rodents, sheep, pigs, and other animals [15][16][17]. However, due to the highly diverse nature of placental species, evidence from animal models derived from human placental diseases is limited [18].…”

Section: Disscussionmentioning

confidence: 99%

Anti-inflammatory effects of Placenta-derived exosomal miR-24-1-5p through targeting TNFAIP8 and its implication in enhancing placental endotoxin tolerance in a model of lipopolysaccharide (LPS)-induced placental inflammation

Zhang

Chen

et al. 2023

Preprint

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Objective and design: Intra-amniotic infection (IAI) represents a potentially catastrophic complication during parturition, with potential for significant maternal and neonatal consequences. As a crucial immunological organ at the maternal-fetal interface, the immunoregulatory capacities of the placenta and the underlying molecular dynamics at play in the pathogenesis of IAI continue to be unclear. Recent studies have found that placenta-derived exosomes (Pd-Exos) and their contained microRNAs (miRNAs) may be involved in immune regulation during pregnancy. This study aimed to investigate the immunoregulatory function of the placenta in connection with the progression of IAI, as well as to elucidate its potential molecular mechanisms. Material or subjects: An IAI model was developed by co-culturing full-term placental explants obtained from elective cesarean sections with lipopolysaccharide (LPS). Using RT-qPCR and ELISA, the dynamic expression patterns of inflammatory factors within the placental explants were identified at varying time points post-LPS exposure. Pd-Exos were then isolated from the culture supernatant of placental explants and subjected to miRNA sequencing to pinpoint exosomal miRNAs integral to the immunoregulatory functions of the placenta. Subsequently, the role of a differentially expressed exosomal miRNA was validated. Results In the early phase of LPS stimulation, extensive pro-inflammatory responses were observed in placental explants, evidenced by the augmented expression of TNF-α and IL-1β. However, as the LPS stimulation progressed to the latter stages, a decrease in the pro-inflammatory response was noted, alongside a gradually surging anti-inflammatory response, signified by a diminishing ratio between pro-inflammatory and anti-inflammatory indicators (TNF-α/IL-10 and IL-1β/IL-1Ra). Following prolonged LPS stimulation of placental explants, the expression of miR-24-1-5p was upregulated in Pd-Exos. Upon internalization by receptor cells (THP-1 cells and Swan 71 cells), miR-24-1-5p can inhibit the expression of its target gene Tumor necrosis factor alpha-induced protein 8 (TNFAIP8), thereby suppressing the expression of downstream inflammatory factors TNF-α and IL-1β. Conclusion Prolonged exposure to the LPS in human term placental tissues induced endotoxin tolerance. Additionally, the placenta-derived exosomal miR-24-1-5p down-regulated the expression of the inflammatory markers TNF-α and IL-1β by inhibiting the functionality of TNFAIP8, thereby contributing to the placental endotoxin tolerance.

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RAGE against the Machine: Can Increasing Our Understanding of RAGE Help Us to Battle SARS-CoV-2 Infection in Pregnancy?

Kurashima

Kendal-Wright

2022

IJMS

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The receptor of advanced glycation end products (RAGE) is a receptor that is thought to be a key driver of inflammation in pregnancy, SARS-CoV-2, and also in the comorbidities that are known to aggravate these afflictions. In addition to this, vulnerable populations are particularly susceptible to the negative health outcomes when these afflictions are experienced in concert. RAGE binds a number of ligands produced by tissue damage and cellular stress, and its activation triggers the proinflammatory transcription factor Nuclear Factor Kappa B (NF-κB), with the subsequent generation of key proinflammatory cytokines. While this is important for fetal membrane weakening, RAGE is also activated at the end of pregnancy in the uterus, placenta, and cervix. The comorbidities of hypertension, cardiovascular disease, diabetes, and obesity are known to lead to poor pregnancy outcomes, and particularly in populations such as Native Hawaiians and Pacific Islanders. They have also been linked to RAGE activation when individuals are infected with SARS-CoV-2. Therefore, we propose that increasing our understanding of this receptor system will help us to understand how these various afflictions converge, how forms of RAGE could be used as a biomarker, and if its manipulation could be used to develop future therapeutic targets to help those at risk.

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High Mobility Group Box 1 in Pig Amniotic Membrane Experimentally Infected with E. coli O55

Cited by 4 publications

References 83 publications

Does Precision-Based Medicine Hold the Promise of a New Approach to Predicting and Treating Spontaneous Preterm Birth?

Does Precision-Based Medicine Hold the Promise of a New Approach to Predicting and Treating Spontaneous Preterm Birth?

Anti-inflammatory effects of Placenta-derived exosomal miR-24-1-5p through targeting TNFAIP8 and its implication in enhancing placental endotoxin tolerance in a model of lipopolysaccharide (LPS)-induced placental inflammation

RAGE against the Machine: Can Increasing Our Understanding of RAGE Help Us to Battle SARS-CoV-2 Infection in Pregnancy?

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