Pretargeting using peptide nucleic acid

Abstract

can adversely influence localization when these molecules are used.F. Chang, Ph.D.METHODS. As RESULTS.In both the infection and tumor mouse models, increased localization of radiolabel was achieved in animals receiving both injectates compared with control animals receiving only the radiolabeled PNA. In the infection model, the infected to normal thigh radioactivity ratio was 3.5 for the study animals compared with 1.7 for control animals (P Å 0.0001). In the tumor model, these values were 1.7 versus 1.2 (P Å 0.003). CONCLUSIONS.We conclude that PNA may be considered an alternative to (strept)avidin and biotin for pretargeting studies.

Telomerase inhibition, oligonucleotides, and clinical trials

“…PNAs, however, are not well distributed in vivo (Rusckowski et al, 1997). For a PNA-based anti-telomerase therapeutic to succeed, the pharmocokinetics and toxicology of PNA would need to be optimized in tandem with surmounting the considerable challenges presented by telomerase as a target.…”

Metal-containing peptide nucleic acid conjugates

“…137 An interesting alternative approach for the use of radioactive PNA probes for therapeutic purposes deserves to be mentioned in this perspective. In the so-called "pretargeting using PNA" developed by Hnatowich et al, 120,127,128 a single-stranded PNA bound to a protein/antibody -which binds, for example, specifically to receptors over-expressed in cancer cells -is administered first into an animal or human, followed by admission of the complementary single-stranded radiolabeled PNA. This technique provides sufficient time for the protein-PNA conjugate to find its target and, for the excess of the construct, to be cleared from circulation and other tissues.…”

“…This technique provides sufficient time for the protein-PNA conjugate to find its target and, for the excess of the construct, to be cleared from circulation and other tissues. 120 Nonetheless, there have been, to the best of our knowledge, no other reports since the three papers of Hnatowich and co-workers despite the huge potential and fascinating perspectives of this concept.…”

Towards the Preparation of Novel Re/99mTc Tricarbonyl-Containing Peptide Nucleic Acid Bioconjugates

“…19,31,[40][41][42][43][44] For the purpose of this work, we decided to use a di-(2-picolyl)amide (Dpam) derivative (3, Figure 1) as a coordination entity for Re/ 99m…”