Pressurized planar electrochromatography as the mode for determination of solvent composition-retention relationships in reversed-phase systems

Dzido, Tadeusz H.; Płocharz, Paweł W.; Klimek-Turek, Anna; Torbicz, Andrzej; Buszewski, Bogusław

doi:10.1556/jpc.21.2008.4.13

Cited by 25 publications

(9 citation statements)

References 13 publications

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“…Despite these advantages, the potential of this method is not currently used in routine analysis, inter alia due to difficulties with the two stages of the PPEC process that precede the development of electrochromatograms: application of samples on the chromatographic plate and prewetting of an adsorbent layer of the chromatographic plate with a mobile phase solution. Our group has designed pre-wetting reservoirs with partitions, which protected the substance zone applied on the adsorbent layer against elution and dispersion during the prewetting process [6, 10, 11, 13]. Disadvantages of this approach are the risk of losing some of the sample and the need for too many manual operations [13].…”

Section: Introductionmentioning

confidence: 99%

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

et al. 2013

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Pressurized planar electrochromatography (PPEC) is a separating technique in which an electric field is applied to force the mobile phase movement through a porous media (electroosmotic effect). High separation efficiency, fast separations and changes in separation selectivity in comparison to liquid chromatography, especially thin layer chromatography (planar chromatography, TLC), are features of this technique. Constructional methodological challenges to PPEC are obstacles to its development and application in laboratory practice. In this article, an attempt to overcome the challenges related to device construction and sample application/injection is described. The introduced device enables both prewetting of the adsorbent layer and electrochromatogram development with a single PPEC device. It also enables simultaneous application/injection of six samples on a chromatographic plate in a stream of the mobile phase (on-line application/injection). In addition, the PPEC chamber was equipped with a thermostat. The device is characterized by an impressive throughput in comparison to the other planar technique, TLC/HPTLC. Although the developed device still needs improvement, it is, in our opinion, a considerable step toward possible automation of this planar separation technique.

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Section: Introductionmentioning

confidence: 99%

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

et al. 2013

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“…The ad van tages such as high per form ance, short sepa ra tion time, and sepa ra tion se lectiv ity dif fer ent than that of liq uid chro ma tog ra phy (LC) make this tech nique very at trac tive for ap pli ca tion in labo ra tory prac tice [17]. In ad di tion, PPEC ex peri ment pro ceeds in a closed sys tem, so no va por phase takes part in the sepa ra tion pro cess [2,3,5,6,7]. Be cause of the features men tioned, PPEC might also be highly suit able for the phar ma ceu ti cal and bio medi cal analy sis [2,8,9,12,19,20].…”

Section: Introductionmentioning

confidence: 99%

Effect of mobile phase buffer pH on separation selectivity of some isoquinoline alkaloids in reversed-phase systems of Pressuriz

Kopciał¹,

Polak²,

Pietraś³

et al. 2015

Curr Issues Pharm Med Sci

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Separation of some isoquinoline alkaloids (narcotine, chelidonine, dihydrocodeine, cinchonine, berberine, cinchonidine, papaverine, apomorphine) has been investigated with pressurized planar electrochromatography (PPEC) and high-performance thin-layer chromatography (HPTLC) in reversed-phase systems. The mobile phase consisted of acetonitrile and aqueous buffer (disodium phosphate and citric acid). The influence of the mobile phase buffer pH on migration distance (PPEC) and retardation factor (HPTLC) of the solutes has been investigated and compared. The results show different separation selectivity in both PPEC and HPTLC systems especially at pH range of buffer solution of the mobile phase that facilitates ionization of the solutes investigated.

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“…First of all the flat profile of the EOF makes the electrochromatographic separations more efficient compared to conventional TLC. Height of theoretical plates obtained in PPEC systems is very low [ 14 , 19 , 21 , 23 ]. Also short time of separation process of solutes with PPEC technique is concerned with EOF velocity of the mobile phase (it is independent on particle diameter of the stationary phase of PPEC systems).…”

Section: Introductionmentioning

confidence: 99%

Influence of the Modifier Type and its Concentration on Electroosmotic Flow of the Mobile Phase in Pressurized Planar Electrochromatography

et al. 2014

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The aim of this work was to find a relationship between electroosmotic flow (EOF) velocity of the mobile phase in pressurized planar electrochromatography (PPEC) and physicochemical properties like zeta potential, dielectric constant, and viscosity of the mobile phase as well as its composition. The study included different types of organic modifiers (acetonitrile, methanol, ethanol, acetone, formamide, N-methylformamide and N,N-dimethylformamide) in the full concentration range. In all experiments, chromatographic glass plates HPTLC RP-18 W from Merck (Darmstadt) were used as a stationary phase. During the study we found that there is no linear correlation between EOF velocity of the mobile phase and single variables such as zeta potential or dielectric constant or viscosity. However, there is quite strong linear correlation between EOF velocity of the mobile phase and variable obtained by multiplying zeta potential of the stationary phase–mobile phase interface, by dielectric constant of the mobile phase solution and dividing by viscosity of the mobile phase. Therefore, it could be concluded that the PPEC system fulfilled the Helmholtz–Smoluchowski equation.

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Pressurized planar electrochromatography as the mode for determination of solvent composition-retention relationships in reversed-phase systems

Cited by 25 publications

References 13 publications

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

Effect of mobile phase buffer pH on separation selectivity of some isoquinoline alkaloids in reversed-phase systems of Pressuriz

Influence of the Modifier Type and its Concentration on Electroosmotic Flow of the Mobile Phase in Pressurized Planar Electrochromatography

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