Pressure related apoptosis in neuronal cell lines

Agar, Ashish; Yip, Sonia S.; Hill, Mark A.; Coroneo, Minas T.

doi:10.1002/(sici)1097-4547(20000515)60:4<495::aid-jnr8>3.0.co;2-s

Cited by 53 publications

(41 citation statements)

References 26 publications

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“…Previous studies in glaucoma have suggested that RGC and axonal loss may be determined by the level of IOP elevation, (14,22,25,30), with evidence that the extent of neuronal apoptosis induced in vitro is determined by the magnitude of the insult (21,29). We next investigated the size of stimulus correlated with the level of apoptosis in our models.…”

Section: Visualization Of Single Nerve Cell Apoptosis In Vivomentioning

confidence: 96%

Real-time imaging of single nerve cell apoptosis in retinal neurodegeneration

Cordeiro

Guo

Luong

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

243

308

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Apoptotic nerve cell death is implicated in the pathogenesis of several devastating neurodegenerative conditions, including glaucoma and Alzheimer's and Parkinson's diseases. We have devised a noninvasive real-time imaging technique using confocal laserscanning ophthalmoscopy to visualize single nerve cell apoptosis in vivo, which allows longitudinal study of disease processes that has not previously been possible. Our method utilizes the unique optical properties of the eye, which allow direct microscopic observation of nerve cells in the retina. We have been able to image changes occurring in nerve cell apoptosis over hours, days, and months and show that effects depend on the magnitude of the initial apoptotic inducer in several models of neurodegenerative disease in rat and primate. This technology enables the direct observation of single nerve cell apoptosis in experimental neurodegeneration, providing the opportunity for detailed investigation of fundamental disease mechanisms and the evaluation of interventions with potential clinical applications, together with the possibility of taking this method through to patients.A poptosis is an orchestrated form of cell ''death by suicide.'' It is essential in both the development and normal maintenance of tissue function. It is also implicated in the pathology of a number of severe neurodegenerative disorders such as glaucoma, motor neuron, and Alzheimer's, Parkinson's, and Huntington's diseases (1). There is evidence that a similar pathogenesis may contribute to neurodegenerative processes in these conditions (2), and that the extent of nerve cell loss is correlated with functional deficit (3-6). If we could directly visualize this process, it would facilitate a much more precise diagnosis and critically enable accurate tracking of the disease state and the action of therapy. However, until now, it has not been possible to detect nerve cell apoptosis in vivo (1, 7-10).Annexin 5 is a protein that, in the presence of Ca 2ϩ , has a high affinity for phosphatidylserine (PS), an anionic phospholipid that is enriched in the inner leaflet of plasma membranes. The externalization of PS from the inner leaflet to the outer layer of the cell membrane is an invariant early feature in the apoptotic process that occurs before DNA fragmentation and nuclear condensation. Because of its properties, FITC-annexin 5 has become widely used in the cytological detection of cells undergoing apoptosis (11). More recently, annexin 5 has been shown to be effective in the identification of apoptosis in vivo by using radiological and macroscopic fluorescent techniques (7,8,10,12,13).However, existing in vivo techniques using annexin 5 either have been unable to resolve the process to a single cellular level (7-9) or require an invasive method performed under terminal anesthesia (10). Imaging the eye, compared with the rest of the body, offers a unique opportunity because of the presence of clear optical media allowing direct visualization of labeled disease processes as they occur. This mea...

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Section: Visualization Of Single Nerve Cell Apoptosis In Vivomentioning

confidence: 96%

Real-time imaging of single nerve cell apoptosis in retinal neurodegeneration

Cordeiro

Guo

Luong

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

243

308

View full text Add to dashboard Cite

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“…The desired gas mix can be pressurized to a constant ambient hydrostatic pressure ranging from 0 to 200 mmHg (28). We applied pressure chamber to visualize directly the infl uence of pressure on the viability, motility, morphology, DNA integrity and sperm fertilizing ability with functional-morphological view.…”

Section: Discussionmentioning

confidence: 99%

“…The pressure chamber used in this study is an established model that allows a gas mix pressurized to a constant ambient hydrostatic pressure ranging from 0-200±2.25 mmHg over the pressure period (28). After pressurization, pressure was restored to atmospheric, and the culture plates removed from the pressure chamber for analysis.…”

Section: Hydrostatic Pressure Treatmentmentioning

confidence: 99%

Effects of hydrostatic pressure on mouse sperm

Karimi¹,

Kamangar²,

Azadbakht³

et al. 2014

BLL

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Abstract:Objectives: The objective of this study was to investigate the abnormalities in sperm after exposure to hydrostatic pressure. Background: Hydrostatic pressure acting on the cells is one of the fundamental environmental mechanical forces. Disorders of relationship between the cells and this mechanical force, such as when pressure varies beyond physiological limits, can lead to disease or pathological states. Sperm exposed to different range of hydrostatic pressure within male reproductive system and after entering the female reproductive system. Methods: Sexually mature male NMRI mice, 8-12 weeks-old were sperm donors. Sperms were separated from the caudal epididymis and maintained in Ham's F-10 culture medium supplemented with 10 % FBS and divided into control and treatments. Sperm suspensions in the treatments were placed within pressure chamber and were subjected to increased hydrostatic pressure of 25, 50 and 100 mmHg (treatment I, II and III) above atmospheric pressure for 2 and 4 h. Sperm viability, motility, morphology, DNA integrity and fertilizing ability were assessed and compared with control. Results: Results showed that hydrostatic pressure dependent on ranges and time manner reduced sperm quality due to adverse effect on viability, motility , morphology, DNA integrity and fertilizing ability in all of treatments, especially after 4h (p<0.05). Conclusion: Our data revealed hydrostatic pressure reduces sperm quality as a consequence of adverse effects on sperm parameters and may cause male infertility or subfertility (Tab. 5, Ref. 5). Text in PDF www.elis.sk.

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“…The unpressurized control cell groups were treated identically within a chamber, but with no pressure applied. The cell culture pressure chamber used in this study is an established model (Agar et al, 2000;Agar et al, 2006) that allows a gas mix to be pressurized to a constant ambient hydrostatic pressure ranging from 0-200 ± 2.25 mmHg over the pressure period. After pressurization, the pressure was restored to the atmospheric value, and the culture plates were removed from the pressure chamber for analysis.…”

Section: Experimental Protocolmentioning

confidence: 99%

“…Namely biological and pharmacological techniques (1), mechanical cues (2), and external mechanical and electrical forces (3) have been used to regulate mMSCs differentiation (Titushkin et al, 2010). Brown (2000) suggested that a significant factor in nerve regeneration is the mechanical environment.…”

Section: Introductionmentioning

confidence: 99%

The effect of hydrostatic pressure on staurosporine-induced neural differentiation in mouse bone marrow‑derived mesenchymal stem cells

Javanmard¹,

Azadbakht²,

Pourmoradi³

2016

BLL

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In this study, the role of hydrostatic pressure on staurosporine-induced neural differentiation in mouse bone marrow mesenchymal stem cells were investigated. The cells were cultured in treatment medium containing 100 nM of staurosporine for 4 hours; then the cells were affected by hydrostatic pressure (0, 25,50, 100 mmHg). The percentage of cell viability by trypan blue staining and the percentage of cell death by Hoechst/PI differential staining were assessed. We obtained the total neurite length. Expression of β-tubulin III and GFAP (Glial fi brillary acidic protein) proteins were also analyzed by immunocytochemistry. The percentage of cell viability in treatments decreased relative to the increase in hydrostatic pressure and time (p < 0.05). The results revealed that total neurite length increased in cells treated with 25 mmHg hydrostatic pressure and decreased in those treated with 100 mmHg hydrostatic pressure (p < 0.05). Low hydrostatic pressure as a mechanical force can improve neural differentiation in mMSCs (mouse mesenchymal stem cells) (Tab. 3, Fig. 7, Ref. 30). Text in PDF www.elis.sk.

show abstract

Pressure related apoptosis in neuronal cell lines

Cited by 53 publications

References 26 publications

Real-time imaging of single nerve cell apoptosis in retinal neurodegeneration

Real-time imaging of single nerve cell apoptosis in retinal neurodegeneration

Effects of hydrostatic pressure on mouse sperm

The effect of hydrostatic pressure on staurosporine-induced neural differentiation in mouse bone marrow‑derived mesenchymal stem cells

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