Effects of hydrostatic pressure on mouse sperm

Abstract

Abstract:Objectives: The objective of this study was to investigate the abnormalities in sperm after exposure to hydrostatic pressure. Background: Hydrostatic pressure acting on the cells is one of the fundamental environmental mechanical forces. Disorders of relationship between the cells and this mechanical force, such as when pressure varies beyond physiological limits, can lead to disease or pathological states. Sperm exposed to different range of hydrostatic pressure within male reproductive system and after entering the female reproductive system. Methods: Sexually mature male NMRI mice, 8-12 weeks-old were sperm donors. Sperms were separated from the caudal epididymis and maintained in Ham's F-10 culture medium supplemented with 10 % FBS and divided into control and treatments. Sperm suspensions in the treatments were placed within pressure chamber and were subjected to increased hydrostatic pressure of 25, 50 and 100 mmHg (treatment I, II and III) above atmospheric pressure for 2 and 4 h. Sperm viability, motility, morphology, DNA integrity and fertilizing ability were assessed and compared with control. Results: Results showed that hydrostatic pressure dependent on ranges and time manner reduced sperm quality due to adverse effect on viability, motility , morphology, DNA integrity and fertilizing ability in all of treatments, especially after 4h (p<0.05). Conclusion: Our data revealed hydrostatic pressure reduces sperm quality as a consequence of adverse effects on sperm parameters and may cause male infertility or subfertility (Tab. 5, Ref. 5). Text in PDF www.elis.sk.

Effect of Foetal Bovine Serum on sperm motility, acrosome reaction and spermatic interaction to zona pellucida in alpacas (Vicugna pacos)

“…The use of foetal bovine serum in the incubation medium of sperm has not been common; for this reason, little is known about the effect of FBS on sperm functionality. However, some studies have used FBS as a supplement in sperm incubation and in vitro fertilization (Chen et al., ; Funahashi & Day, ,b; Goel et al., ; González‐Fernández et al., ; Karimi et al., ; Keskintepe et al., ; Leibfried‐Rutledge et al., ; Tian et al., ; Yang et al., ).…”

“…However, it has been recognized that the presence of FBS results in higher motility in post-cryopreserved sperm increases the number of embryos and enhances the acrosome reaction in several species (Chen, Doncel, Alvarez, & Acosta, 1995;Dong, Huang, & Tiersch, 2006;Funahashi & Day, 1993a;Garzón et al, 2008;Goel et al, 2016;Karimi, Kamangar, Azadbakht, Amini, & Amiri, 2014;Keskintepe, Luvoni, Rzucidlo, & Brackett, 1996;Koh, Yokoi, Tsuji, Tsuchihashi, & Ohta, 2010;Kwon et al, 2014;Long et al, 1996;Rizal & Sangadji, 2013;Tian et al, 2014;).…”

Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets

“… 13 , 14 However, HP beyond the normal range, also being classified as pathological HP, can lead to decompensated lesions in tissues and organs, such as bladder fibrosis and decreased sperm quality. 15 Osteogenesis and bone density can also be enhanced by HP. 13 Additionally, HP can change the conformation of ion channels and regulate ion transmembrane transportation, 16 thereby affecting pathophysiological processes.…”