Preserved Bone Allografts in Reconstructive Orthopaedics

Komender, J; KOMENDER, A.; Malczewska, Hanna; Marczyński, Wojciech

doi:10.1142/9789812776976_0012

Cited by 11 publications

(13 citation statements)

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“…Although these remain widely available and offer no donor site morbidity, there are notable risks and important shortcomings associated with their use. 5,6,7,8 Allogenic grafts carry the risk of resorption, immune rejection, and disease transmission. Synthetic grafts may be complicated by infection and extrusion.…”

Section: Fundamentals Of Tissue Engineeringmentioning

confidence: 99%

5 Fundamentals of Tissue Engineering

Papel¹,

Frodel²,

Holt³

et al. 2016

Facial Plastic and Reconstructive Surgery

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Section: Fundamentals Of Tissue Engineeringmentioning

confidence: 99%

5 Fundamentals of Tissue Engineering

Papel¹,

Frodel²,

Holt³

et al. 2016

Facial Plastic and Reconstructive Surgery

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“…The limited amount of tissue possible to obtain in children, surgical procedures conducted during its collection and, additionally -in case of bone graft -the risk of no graft adhesion with the host's tissue and its resorption, emphasise the imperfection of this method (Rotter et al, 2005). Despite common use of allogeneic cartilage and bone in regeneration of defects, the limiting factor may constitute their availability and possibility of losing them (Komender et al, 2001;Więcko et al, 1980). Chondrocytes amplified in vitro and used in tissue engineering may undergo differentiation (Barbero et al, 2003).…”

Section: Xenogeneic Engraftment Of Antlerogenic Cells and Tissue Regementioning

confidence: 99%

Characteristics of Antlerogenic Stem Cells and Their Potential Application

Cegielski¹,

Iżykowska²,

Dziewiszek³

et al. 2010

Tissue Engineering

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“…The repair of craniofacial cartilaginous defects created by trauma, previous surgical resection, and congenital deformities requires analogous reconstructive material to obtain optimal results. Unfortunately, the use of synthetic grafts may be complicated by infection and extrusion, whereas allogenic grafts carry the risk of immune rejection and disease transmission . Autologous cartilage grafts as an alternative can be harvested from the nasal septum, auricle, and rib.…”

Section: Introductionmentioning

confidence: 99%

Effect of hyaluronidase on tissue-engineered human septal cartilage

et al. 2016

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Objectives Structural properties of tissue-engineered cartilage can be optimized by altering its collagen to sulfated glycosaminoglycan (sGAG) ratio with hyaluronidase. The objective was to determine if treatment of neocartilage constructs with hyaluronidase leads to increased collagen:sGAG ratios, as seen in native tissue, and improved tensile properties. Study Design Prospective, basic science Methods Engineered human septal cartilage from 12 patients were treated with hyaluronidase prior to culture. Control and treated constructs were analyzed at 3, 6, or 9 weeks for their biochemical, biomechanical, and histological properties. Results Levels of sGAG were significantly reduced in treated compared to control constructs at 3, 6 and 9 weeks. Treated constructs had higher collagen:sGAG ratios when compared with control constructs at 3, 6, and 9 weeks. Treated constructs had greater tensile strength, strain at failure as well as increased stiffness as measured by the equilibrium and ramp tensile moduli when compared with the untreated control constructs. Continued time in culture improved tensile strength in both treated and control constructs. Conclusions Hyaluronidase treatment of engineered septal cartilage decreased total sGAG content without inhibiting expansive growth of the constructs. Decreased sGAG in treated constructs resulted in increased collagen to sGAG ratios and was associated with an increase in tensile strength and stiffness. With additional culture time, sGAG increased modestly in depleted constructs, and some initial gains in tensile properties were dampened. Alterations in the dosage of hyalurondiase during neocartilage fabrication can create constructs that have improved biomechanical properties for eventual surgical implantation. Level of Evidence NA

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Preserved Bone Allografts in Reconstructive Orthopaedics

Cited by 11 publications

References 0 publications

5 Fundamentals of Tissue Engineering

5 Fundamentals of Tissue Engineering

Characteristics of Antlerogenic Stem Cells and Their Potential Application

Effect of hyaluronidase on tissue-engineered human septal cartilage

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