Preservation of the naïve features of mesenchymal stromal cells in vitro: Comparison of cell- and bone-derived decellularized extracellular matrix

Abstract

The fate and behavior of bone marrow mesenchymal stem/stromal cells (BM-MSC) is bidirectionally influenced by their microenvironment, the stem cell niche, where a magnitude of biochemical and physical cues communicate in an extremely orchestrated way. It is known that simplified 2D in vitro systems for BM-MSC culture do not represent their naïve physiological environment. Here, we developed four different 2D cell-based decellularized matrices (dECM) and a 3D decellularized human trabecular-bone scaffold (dBone) to evaluate BM-MSC behavior. The obtained cell-derived matrices provided a reliable tool for cell shape-based analyses of typical features associated with osteogenic differentiation at high-throughput level. On the other hand, exploratory proteomics analysis identified native bone-specific proteins selectively expressed in dBone but not in dECM models. Together with its architectural complexity, the physico-chemical properties of dBone triggered the upregulation of stemness associated genes and niche-related protein expression, proving in vitro conservation of the naïve features of BM-MSC.

Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization

“…They are the simplest models, easy to setup, and effective for mechanistic studies. However, the results from monolayer culture models may not represent the features of complex 3D cell-cell and cell-matrix interactions 35 and their impact on the tissue response to bacterial challenge. Therefore, in this study, we developed a 3D culture model to represent the gingival and periodontal connective tissue elements of the dento-gingival unit, respectively.…”

Advances Focusing on the Application of Decellularized Extracellular Matrix in Periodontal Regeneration

“…The fate and behavior of mesenchymal stem cells are influenced by the stem cell niches ideal biochemical and physical cues. Ana Rita Pereira et al compared the biological behaviors of BMSCs when exposed to C-dECM and T-dECM, and better outcomes were observed in 3D decellularized bone tissue for greater architecture complexity and physicochemical properties [ 52 ]. To sum up, tissue-derived dECM has great potential in the context of endogenous periodontal regeneration, with a better effect on preserving the tissue niche intended for different tissues in periodontal defects.…”

Next generation of neurological therapeutics: Native and bioengineered extracellular vesicles derived from stem cells

“…Firstly, from a pharmaceutical perspective, the diversity of production methods makes it more challenging to control the quality of SC-EVs during the manufacturing process. Secondly, all the subtle variations in parent cell culture conditions (cell passaging, cell inoculation density), time and frequency of EVs collection, different EVs isolation and purification methods, EVs storage conditions will vary the composition of SC-EVs [111] , [112] , [113] . Finally, the uniqueness of EVs size makes the process of sterilization and/or removal of additives more challenging [114] .…”