Preservation of myocyte structure and mitochondrial integrity in subzero cryopreservation of mammalian hearts for transplantation using antifreeze proteins—an electron microscopy study

Amir, Gabriel; Rubinsky, Boris; Kassif, Yigal; Horowitz, Liana; Smolinsky, Aram; Lavee, Jacob

doi:10.1016/s1010-7940(03)00306-3

Cited by 57 publications

(25 citation statements)

References 16 publications

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“…Success of vitrification method is still disrupted as quality of embryo post warming decreses (Amir et al, 2013). Decreased embryo quality post warming highly influences embryo implantation rate which in turn will decrease gestation rate.…”

Section: Introductionmentioning

confidence: 99%

“…Insulin Transferrin Selenium is a complex protein which is able to stimulate cell growth, prevent cell damage due to role of anti oxidant in it so that it can maintain embryo viability post thawing.According to et al, (2007) and Amir et al, (2013), Insulin Transferrin Selenium is able to increase quality and viability of blastocyst resulted from in vitro culture.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Insulin Transferrin Selenium Administration on Rat's Cultured In Vitro Embryo Post Warming After being Frozen using Vitrification Method

Widjiati¹,

Luqman²,

Hendrawan³

et al. 2018

Proceedings of the 1st International Conference in One Health (ICOH 2017)

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Success of embryo transfer is determined by availability of a plenty of amount of embryo stock. To make embryo stock remain in good quality, it is necessary to have an easy, cheap, simple and effective storage method. One of the storage methods is vitrification.Success of vitrification method is still disrupted due to decreased embryo quality post warming. Decreased embryo viability influences high implantation rate and gestation. Low implantation rate will result in low gestation rate. Therefore, study is needed to optimize vitrification medium so that it will be able to optimize cryoprotectant role to protect embryo due to temperature stressor in vitrification method.Administering Insulin Transferrin Selenium on vitrification medium is able to bind free radicals caused by temperature stressor due to frozen Insulin Transferrin Selenium which is a complex protein that is able to stimulate cell growth, prevent cell damage due to anti oxidant role in it so that it is able to maintain embryo viability post thowing. Insulin Transferrin Selenium is able to increase quality and viability of blastocyst resulted from in vitro culture.The study aims to prove Insulin Transferrin Selenium supplementation on vitrification medium is able to increase viability of rat embryo post warming. Steps of the research cover oocyte collection, embryo vitrification, warming, and in vitro culture. In vitro culture yields morula embryo with excellent quality and fits to be frozen using vitrification method.Insulin Transferin Selenium administration of 5,10 and 15 g/ml is able to increase embryo viability up to 100 %. Frozen embryo post warming and recultured for 5 hours , viability of morula embryo ofthe treatment group administered by Insulin Transferrin Selenium reached 73.4 % -83.4% whereas control group without being administered by InsulinTransferrin Selenium reached only 64.7 %. The research concludes that Insulin Transferrin Selenium.administration is able to increase viability of rat embryo at morula stage.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Insulin Transferrin Selenium Administration on Rat's Cultured In Vitro Embryo Post Warming After being Frozen using Vitrification Method

Widjiati¹,

Luqman²,

Hendrawan³

et al. 2018

Proceedings of the 1st International Conference in One Health (ICOH 2017)

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“…The authors found that AFGPs were deleterious to the isolated rat hearts in a dose-dependent manner exacerbating the damage caused by freezing. Amir et al (2003) reported successful subzero preservation of mammalian hearts using fish AFP I and AFP III in an in vivo heterotopic heart transplantation model. All hearts preserved at subzero temperatures using AFP I or AFP III survived, displaying good to excellent viability scores.…”

Section: Introductionmentioning

confidence: 99%

“…Electron microscopy studies demonstrated that antifreeze proteins prevented the damage caused by freezing, which correlated well with the post-transplant hemodynamic heart performance. This group followed up on this study in which hearts were preserved at −1.1 to −1.3°C for 6 h (Amir et al 2003) with another study in which they evaluated whether lowering the temperature could increase the preservation time of a mammalian (rat) heart and whether prolonged subzero preservation using AFPs may improve the viability of harvested hearts compared to hearts preserved using standard techniques at +4°C (Amir et al 2004). They were able to demonstrate, using an in vitro Langendorff perfusion system, that AFPs prevent freezing and improve survival in prolonged subzero preservation of rat hearts.…”

Section: Introductionmentioning

confidence: 99%

Lessons from nature for preservation of mammalian cells, tissues, and organs

Brockbank

Campbell

Greene

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

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The study of mechanisms by which animals tolerate environmental extremes may provide strategies for preservation of living mammalian materials. Animals employ a variety of compounds to enhance their survival, including production of disaccharides, glycerol, and antifreeze compounds. The cryoprotectant glycerol was discovered before its role in amphibian survival. In the last decade, trehalose has made an impact on freezing and drying methods for mammalian cells. Investigation of disaccharides was stimulated by the variety of organisms that tolerate dehydration stress by accumulation of disaccharides. Several methods have been developed for the loading of trehalose into mammalian cells, including inducing membrane lipid-phase transitions, genetically engineered pores, endocytosis, and prolonged cell culture with trehalose. In contrast, the many antifreeze proteins (AFPs) identified in a variety of organisms have had little impact. The first AFPs to be discovered were found in cold water fish; their AFPs have not found a medical application. Insect AFPs function by similar mechanisms, but they are more active and recombinant AFPs may offer the best opportunity for success in medical applications. For example, in contrast to fish AFPs, transgenic organisms expressing insect AFPs exhibit reduced ice nucleation. However, we must remember that nature's survival strategies may include production of AFPs, antifreeze glycolipids, ice nucleators, polyols, disaccharides, depletion of ice nucleators, and partial desiccation in synchrony with the onset of winter. We anticipate that it is only by combining several natural low temperature survival strategies that the full potential benefits for mammalian cell survival and medical applications can be achieved.

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“…Antifreeze proteins can aid in the cryo-preservation of cells, tissues, organs and embryos, especially in transplant organs (Amir et al, 2003;Xu et al, 2004;Xie et al, 2005). In addition, genetic engineering of the antifreeze protein was applied to improve some plant's antifreezing ability (Worrall et al, 1998;Zhu and Da, 2003).…”

Section: Introductionmentioning

confidence: 99%

Cloning, sequencing and prokaryotic expression of cDNAs for antifreeze protein family from beetle Tenebrio molitor

et al. 2008

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Partial cDNA sequences coding for antifreeze proteins in Tenebrio molitor were obtained by RT-PCR. Sequence analysis revealed nine putative cDNAs with a high degree of homology to Tenebrio molitor antifreeze protein genes published in GenBank. The recombinant pGEX-4T-1-tmafp-XJ430 was introduced into E. coli BL21 to induce a GST fusion protein by IPTG. SDS-PAGE analysis for the fusion protein shows a band of 38 kDa. pCDNA3-tmafp-XJ430 was injected into mice to generate antiserum which was later detected by indirect ELISA. The titer of the antibody was 1:2000. Western blotting analysis shows that the antiserum was specifically against the antifreeze protein. Our results laid the foundation for further studies on the properties and functions of insect antifreeze proteins.

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Preservation of myocyte structure and mitochondrial integrity in subzero cryopreservation of mammalian hearts for transplantation using antifreeze proteins—an electron microscopy study

Abstract: Subzero cryopreservation of mammalian hearts for transplantation using AFP I or AFP III is feasible with preservation of myocyte structure and mitochondrial integrity.

Cited by 57 publications

References 16 publications

Effect of Insulin Transferrin Selenium Administration on Rat's Cultured In Vitro Embryo Post Warming After being Frozen using Vitrification Method

Effect of Insulin Transferrin Selenium Administration on Rat's Cultured In Vitro Embryo Post Warming After being Frozen using Vitrification Method

Lessons from nature for preservation of mammalian cells, tissues, and organs

Cloning, sequencing and prokaryotic expression of cDNAs for antifreeze protein family from beetle Tenebrio molitor

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