Preservation of mithun (Bos frontalis) semen at refrigeration temperature

Karunakaran, M.; Dhali, A.; Mech, A.; Khate, K.; Rajkhowa, C.; Mishra, Dipendra

doi:10.1016/j.anireprosci.2006.09.011

Cited by 19 publications

(27 citation statements)

References 10 publications

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“…The colour of semen was creamy white in freezable ejaculates and watery white in non-freezable ejaculates (Table 1). Similar observation was reported in earlier report in Mithun species (Karunakaran et al, 2007). The volume of ejaculate was significantly (p< 0.05) higher in freezable than in non-freezable ejaculates (Table 1).…”

Section: Resultssupporting

confidence: 80%

“…Mass activity of the ejaculates was positively correlated (p< 0.05) with sperm motility, liveability, intactness of acrosome, nuclear integrity and vanguard distance travelled by sperm and negatively correlated (p< 0.05) with total morphological abnormality in freezable ejaculates (Table 2) whereas in non-freezable ejaculates it was positively correlated (p< 0.05) with sperm viability and integrity of plasma membrane (Table 3). The present observation was similar with report submitted by Karunakaran et al (2007) and Bhattacharyya et al (2009). However, Bhattacharyya et al (2005) reported lower than present value.…”

Section: Resultssupporting

confidence: 78%

“…The volume of ejaculate was significantly (p< 0.05) higher in freezable than in non-freezable ejaculates (Table 1). Volume of ejaculate in the present study was higher than earlier reports by Bhattacharyya et al (2005) and Karunakaran et al (2007). But volume was lower than semen was collected through artificial vagina method of semen collection in Mithun (Bhattacharyya et al, 2009;Kumar and Bhattacharyya, 2009;Mondal et al, 2010).…”

Section: Resultscontrasting

confidence: 56%

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Semen Quality Parameters of Freezable and Non-Freezable Ejaculates of Mithun (Bos frontalis) Bulls

Perumal¹

2015

Adv. Anim. Vet. Sci.

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INTRODUCTIONM ithun (Bos frontalis) is a "mountain cattle" living as a semi-wild free-range in the North-Eastern Hill (NEH) region of India and responsible for the socio-cultural and economic status of the tribal population (Simoons, 1984). Latest livestock census of India (2007) indicated Mithun density and population is decreasing and is mainly due to lack of proper breeding management in the tribal regions. So proper and scientific interventions are needed from all quarters to conserve and preserve the Mithun population to enhance the socio-economic-cultural status of the tribes. Natural breeding is followed in Mithun, Research ArticleAbstract | A study was designed to measure the semen quality parameters (SQP) such as volume, colour, concentration, mass activity, percent of sperm motility, viability, total morphological abnormality, intactness of acrosome, plasma membrane, nucleus, vanguard distance travelled by sperm in bovine cervical mucus and hydrogen ion concentration in freezable and non-freezable ejaculates of Mithun. Fifty ejaculates were collected from ten matured Mithun bulls and split to freezable and non-freezable ejaculates based on the post thaw motility (40% or more considered as freezable ejaculates). The result has shown that SQP differed significantly (P< 0.05) between the freezable and non-freezable ejaculates and freezable ejaculates have significantly (P< 0.05) higher value than the non-freezable ejaculates. However, morphological abnormality and pH were significantly (P< 0.05) higher in non-freezable than in freezable ejaculates. Mass activity was positively (P< 0.05) correlated with motility, liveability, integrity of acrosome, plasma membrane, nucleus and BCMPT and negatively correlated (P< 0.05) with morphological abnormality. Concluded that SQPs were significantly higher in freezable than in non-freezable ejaculates and indicates freezable sperm has higher structural stability than the non-freezable sperm caused freezable sperm.

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Section: Resultssupporting

confidence: 80%

Section: Resultssupporting

confidence: 78%

Section: Resultscontrasting

confidence: 56%

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Semen Quality Parameters of Freezable and Non-Freezable Ejaculates of Mithun (Bos frontalis) Bulls

Perumal¹

2015

Adv. Anim. Vet. Sci.

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“…In the current investigation, the qualitative parameters of the semen samples were comparable with the previous studies in mithun (Bhattacharya et al, 2005;Karunakaran et al, 2006). In our study, the percentage of progressively motile spermatozoa in cryopreserved samples was found to be comparatively lower than cattle bull.…”

Section: Discussionsupporting

confidence: 79%

“…cryopreserved gaur semen is capable of establishing pregnancy in cow through AI (Sukwongs et al, 1998). Previous reports indicate that the different qualitative parameters of mithun semen are within the normal range and may be compared with cattle (Bhattacharya et al, 2005;Karunakaran et al, 2006). The recent initiatives to popularize mithun as an economic beef animal emphasize the introduction of control breeding and breed improvement programme in this species.…”

Section: Introductionmentioning

confidence: 99%

Birth of the first mithun (Bos frontalis) calf through artificial insemination

Dhali

Karunakaran

Mech

et al. 2008

Animal

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The study describes the standardization of a suitable semen cryopreservation protocol for the first time in mithun (Bos frontalis) and birth of the first mithun calf through artificial insemination. The semen samples were collected from adult bulls through the rectal massage method and cryopreserved in liquid nitrogen using tris-egg yolk-glycerol diluent. The diluted semen samples were packaged in 0.50 ml straws and kept at 58C for 4 h for equilibration. Following the equilibration, the straws were frozen into liquid nitrogen vapour for 10 min and then plunged into liquid nitrogen for storage. It was observed that the progressive motility (%) decreased significantly (P , 0.01) in cryopreserved semen (43.3 6 4.1) compared with fresh samples (76.6 6 3.3). The percentages of live spermatozoa (P , 0.01) and spermatozoa with intact acrosome (P , 0.05) also decreased significantly in cryopreserved semen (54.0 6 3.3 and 64.6 6 5.3) compared with fresh samples (79.3 6 2.6 and 85.3 6 1.8). Simultaneously, the total morphological abnormality (%) was found to be significantly (P , 0.01) higher in cryopreserved samples (15.46 6 2.68) than in fresh semen (3.85 6 0.63). A total of three mithun cows were inseminated using the cryopreserved semen. All the cows conceived following insemination and gave birth to healthy calves. The study revealed that mithun semen can be cryopreserved efficiently using tris-egg yolk-glycerol diluent, which can be further used for artificial insemination.

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