Preservation of FGF-2 bioactivity using heparin-based nanoparticles, and their delivery from electrospun chitosan fibers

Volpato, Fabio Zomer; Almodóvar, Jorge; Erickson, Kristin; Popat, Ketul C.; Migliaresi, Claudio; Kipper, Matt J.

doi:10.1016/j.actbio.2011.12.023

Cited by 118 publications

(116 citation statements)

References 29 publications

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“…In such a way, the release of the therapeutics would be attenuated, and the functionality of the surface-immobilized biomolecules could be preserved. 57 This strategy is usually applied to solve the problem of initial burst release as well as short release time. 58 However, when the target biomolecule is chemically immobilized onto the surface, it would hardly be released.…”

Section: Electrospinningmentioning

confidence: 99%

“…So this technique would be more applicable for gene or growth-factor delivery, where a slow and prolonged release of the therapeutic agent is required. 57,[59][60][61][62][63] For biomolecules like DNA, growth factors (GFs), or enzymes that lose bioactivity and degrade within a few days, conjugating the biomolecules to the fiber surfaces and slowly releasing them into the nearby tissue would significantly preserve their functionality.…”

Section: Electrospinningmentioning

confidence: 99%

“…110 Although the controlled release of GFs is achievable, the instability of GFs hinders the successful development of GF-loaded tissue-engineering scaffolds. Various techniques, such as blending, 91,111 specific or nonspecific surface modifications, 57,[60][61][62][63]105 coaxial electrospinning, 56 emulsion electrospinning, 79,88 and combination of electrospinning with other conventional techniques, 112,113 were applied for GF incorporation into nanofibrous scaffolds, yielding varied level of success. Burst release of EGF was obtained from silk nanofibers blended with EGF, due to the hydrophobic nature of EGF.…”

Section: 108mentioning

confidence: 99%

“…91 However, a surface-modified nanofibrous scaffold was more appropriate for GF delivery, mainly due to its morphological and biochemical similarity to native tissues. 57 Among the different substances with capability of conjugation with GFs, heparin remains the perfect choice, since it provides a more mimicked native ECM composition with the ability to efficiently interact with GF via negatively charged sulfate groups on its backbone. 57,63 Moreover, polysaccharides that are found in proteoglycans (eg, aggrecan) of mammalian tissues are able to conjugate to GFs in a similar way to natural ECM.…”

Section: 108mentioning

confidence: 99%

“…57 Among the different substances with capability of conjugation with GFs, heparin remains the perfect choice, since it provides a more mimicked native ECM composition with the ability to efficiently interact with GF via negatively charged sulfate groups on its backbone. 57,63 Moreover, polysaccharides that are found in proteoglycans (eg, aggrecan) of mammalian tissues are able to conjugate to GFs in a similar way to natural ECM. Mimicking the native tissues, attempts have been made to immobilize GF onto scaffolds using polysaccharides and/or heparin.…”

Section: 108mentioning

confidence: 99%

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Advances in drug delivery via electrospun and electrosprayed nanomaterials

Zamani¹,

Prabhakaran²,

Ramakrishna³

2013

IJN

184

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Electrohydrodynamic (EHD) techniques refer to procedures that utilize electrostatic forces to fabricate fibers or particles of different shapes with sizes in the nano-range to a few microns through electrically charged fluid jet. Employing different techniques, such as blending, surface modification, and coaxial process, there is a great possibility of incorporating bioactive such molecules as drugs, DNA, and growth factors into the nanostructures fabricated via EHD techniques. By careful selection of materials and processing conditions, desired encapsulation efficiency as well as preserved bioactivity of the therapeutic agents can be achieved. The drug-loaded nanostructures produced can be applied via different routes, such as implantation, injection, and topical or oral administration for a wide range of disease treatment. Taking advantage of the recent developments in EHD techniques like the coaxial process or multilayered structures, individually controlled delivery of multiple drugs is achievable, which is of great demand in cancer therapy and growth-factor delivery. This review summarizes the most recent techniques and postmodification methods to fabricate electrospun nanofibers and electrosprayed particles for drug-delivery applications.

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Section: Electrospinningmentioning

confidence: 99%