Preservation of female genetic resources of common carp through oogonial stem cell manipulation

Franěk, Roman; Tichopád, Tomáš; Steinbach, Christoph; Xie, Xun; Lujić, Jelena; Marinović, Zoran; Horváth, Ákos; Kašpar, Vojtěch; Pšenička, Martin

doi:10.1016/j.cryobiol.2019.01.016

Cited by 25 publications

(21 citation statements)

References 37 publications

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“…We can conclude, that previously reported 40-60% transplantation success of testicular [29] and ovarian [30] GSC in common carp is corresponding with numbers of adult germline chimeras producing donor-derived progeny in our study. Therefore, we can retrospectively say, that transplantation success assessed 2-3 months post transplantation probably gives reliable numbers about expected numbers of adult chimeras.…”

Section: Discussionsupporting

confidence: 86%

“…Gold sh (Carassius auratus) broodstock for recipients production originated from the same resource as in our previous studies [29,30]. Gold sh gametes were obtained after hormonal stimulation.…”

Section: Recipient Production and Transplantationmentioning

confidence: 99%

“…1C). Small fraction of dissociated tissue was separated before the addition of L15 with FBS and was used for PKH26 staining as described previously [30]. Recipients receiving labelled cells were reared separately and were not reproduced.…”

Section: Recipient Production and Transplantationmentioning

confidence: 99%

“…Protocols for cryopreservation of common carp male [29] and female [30] gonadal tissue containing a population of pluripotent GSCs have been developed recently in our laboratory as the rst step towards novel reproductive biotechnologies development in carp.…”

Section: Introductionmentioning

confidence: 99%

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Production of common carp donor-derived offspring from goldfish surrogate broodstock

Franěk

Kašpar

Gela

et al. 2020

Preprint

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Background: Common carp is the fourth most-produced species in worldwide aquaculture. Significant efforts are invested in breeding and preservation of genetic integrity of this important species. However, maintaining carp gene bank in situ can be considered as demanding due to its big body size. Recent progress in reproductive biotechnologies in fish allows improving some unfavourable characteristics of a target species using surrogate reproduction. Germ stem cells (gamete precursors) from one species are transplanted into different surrogate species with small body size. After maturation, surrogates are producing donor-derived progeny. Efficient protocols for cryopreservation of carp male and female germ stem cells have been developed lately. Thus, the next logical goal was to assess the potential of goldfish surrogate to produce donor-derived gametes of common carp after intraperitoneal transplantation of testicular cells. Results: High transplantation success was achieved when 44% of the surviving goldfish produced pure donor-derived gametes of common carp. More importantly, both viable eggs and sperm giving rise to pure common carp progeny were produced, witnessing sustainability of the presented method. Donor-derived identity of the offspring was confirmed by genotyping and typical phenotype corresponding to the donor species. Reproductive performance of chimeras was similar to goldfish controls. Assessment of gamete characteristics showed that the size of donor-derived eggs is between control carp and goldfish eggs. Interestingly, flagellum length in donor-derived spermatozoa was comparable to common carp flagellum and significantly shorter than goldfish flagellum. Conclusions: In this study, we succeeded in the production of pure common carp progeny from surrogate goldfish recipients transplanted intraperitoneally by testicular germ cells. Here we reported production of viable eggs between most distant species up to date. Good reproductive performance of goldfish germline chimeras gives a promising prospect for further analysis about the long-term reproductive performance of surrogates, recovery of cryopreserved germ cells or production of monosex stocks. Presented technology is ready to ease needs for carp breeds preservation and their recovery using many times smaller goldfish surrogates.

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Section: Discussionsupporting

confidence: 86%

Section: Recipient Production and Transplantationmentioning

confidence: 99%

Section: Recipient Production and Transplantationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production of common carp donor-derived offspring from goldfish surrogate broodstock

Franěk

Kašpar

Gela

et al. 2020

Preprint

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“…Declarations of interest: none 28 2 1 Introduction: 29 Surrogate reproduction in fish via intraperitoneal germ cell transplantation is a promising 30 technology for aquaculture as well preservation of endangered species when recipient species with 31 more favorable characteristics can be used [1]. So far germ cell technologies are being applied in 32 wide range of fish species such as, salmonids [2,3], cyprinids [4,5], Nile tilapia [6-8], medaka [9], 33 sturgeons [10][11][12] or several marine fish species [13,14]. One from the main prerequisites for 34 successful surrogate propagation is the host's sterility ensuring no contamination of donor-derived 35 gametes during reproduction [2] and very likely lower competition for space in gonads between 36 endogenous germ cells and introduced exogenous germ cells resulting in higher production of 37 donor-derived gametes.…”

mentioning

confidence: 99%

Production and use of triploid zebrafish for surrogate reproduction

Franěk

Tichopád

Fučíková

et al. 2019

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12We report for first time comparison of two approaches for zebrafish triploid production using cold 13 shock and heat shock treatment. Subsequently, produced triploid zebrafish were used as a recipients 14 for intraperitoneal transplantation of ovarian and testicular cells originating from vas:EGFP strain in 15 order to verify their suitability for surrogate reproduction. Heat shock treatment was far more 16 effective evaluated as success rate of triploid production and viability in comparison to cold shock. 17Triploids were produced with up to 100% efficiency in particular females. As expected, all triploids 18 were males. Subsequently, germ cells transplantation revealed that triploids are suitable surrogate 19 hosts. Production of donor-derived sperm was achieved in 23% and 16% of triploids transplanted 20 by testicular and ovarian cells, respectively. Success of the transplantation was confirmed by 21 positive GFP signal detected in gonads of dissected fish and stripped sperm. Germline transmission 22

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Development of an Effective Cryopreservation Protocol for Blue Catfish Oogonia

et al. 2021

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Long‐term storage of oogonia and germ‐line stem cells provides an alternative to the limitations associated with cryopreserving eggs of important fish species. These cell types are less vulnerable to the stresses of freezing. Cryopreservation has enormous potential for aquaculture advancement, but protocols must be developed for each species and cell type since its success hinges on various input factors. Blue Catfish Ictalurus furcatus were selected as the test species in this study because of the need to improve fry production of Blue Catfish ♂ × Channel Catfish I. punctatus ♀ hybrids, which can be facilitated by storing oogonia in gene banks. Our objective was to develop a freezing protocol for oogonia of this species. We tested different permeating and nonpermeating cryoprotectants, concentrations of these agents, and freezing rates. We proved that all three factors influenced postthaw recovery of oogonia. Of the permeating cryoprotectants, 1.0 M dimethyl sulfoxide resulted in the most live cells with the highest viability percentages, and adding 0.2 M lactose with 10% egg yolk further improved the results. There were also specific interactions in which the effects of concentration and freezing rate varied among the cryoprotectant treatments. The most effective freezing rate was −1.0°C/min, and cell viability was reduced at −2.5°C/min and −5.0°C/min. From these results, we propose adding 1.0 M dimethyl sulfoxide with 0.2 M lactose and 10% egg yolk to cryomedia and freezing it at a rate of −1.0°C/min. By developing a cryopreservation protocol for a commonly cultured catfish, this work may guide the development of protocols for other species of interest.

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Preservation of female genetic resources of common carp through oogonial stem cell manipulation

Cited by 25 publications

References 37 publications

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Production and use of triploid zebrafish for surrogate reproduction

Development of an Effective Cryopreservation Protocol for Blue Catfish Oogonia

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