Preservation of canine and feline corneoscleral tissue in Optisol^® GS

Abstract

Corneoscleral tissue of cats survived longer, when preserved in Optisol GS at 4 degrees C, than that of dogs. Light and scanning electron microscopy revealed good preservation of the endothelial cell layer for up to 10 days in dogs and up to 15 days in cats.

Potentiation of Femtosecond Laser Intratissue Refractive Index Shaping (IRIS) in the Living Cornea with Sodium Fluorescein

“…It maintains epithelial, stromal, and endothelial viability for up to 14 days after corneal extraction 31,32. A previous study of feline corneoscleral tissue found that Optisol-GS maintained a functional endothelial cell layer for up to 15 days postmortem,33 suggesting similar effectiveness for corneas from different species. However, to maximize corneal health in our experiments, IRIS was performed within a few hours of corneal removal.…”

Subjective vs. objective analysis of the corneal endothelial cells in the rabbit cornea by scanning electron microscopy – a comparison of two different methods of corneal fixation

“…Similarly, following various types of laboratory studies it is not uncommon for the methods to then simply state that the corneas were fixed. Although some of these preparations have included a scleral rim with the cornea, 21 the point is that the corneas were fixed without the influence of the intraocular pressure to preserve the overall shape and 3‐D architecture of the cornea. Alternatively, corneas have been prepared for endothelial assessment with the initial fixation step based on immersion of the globe (eyeball) in fixative, 8,22 sometimes after making an incision at the limbus, or at the equator of the enucleated eye.…”

“…If the cell surfaces were not flat, then a darker region within the cell boundaries would indicate a local irregularity (i.e., a raised area or depressed area) and if the entire cell or group of cells were darker then this could indicate cellular swelling (edema) 23 . If the magnification of the specular microscope is high, then it is further possible to consider whether the cell–cell borders (i.e., the lines between each of the cell apices) are reasonably straight (as they should be) or if they were rounded (as can occur if there are some unusually large and irregular cells present) 15,21 . All of these aspects of the overall appearance of the cell layer and the cells surely need to be assessed as part of considering whether the SEM appearance is a reasonable illustration of the in vivo appearance.…”

Early postoperative results of Descemet’s stripping endothelial keratoplasty in six dogs with corneal endothelial dystrophy

“…This incision was extended along the entire limbus. A stab incision was then made into the ciliary body with a scalpel, and the final excision made with Westcott scissors . Under sterile conditions, the corneoscleral tissue was then placed in a unit with the endothelium uppermost.…”

“…Under sterile conditions, the corneoscleral tissue was then placed in a unit with the endothelium uppermost. The unit contained 20 mL of Optisol GS (Bausch & Lomb Australia, Chatswood, NSW, Australia) and was stored at 4°C . Transplantation of the cornea took place within 24 hours following globe retrieval.…”