Preservation of biomaterials and cells by freeze-drying: Change of paradigm

Merivaara, Arto; Zini, Jacopo; Koivunotko, Elle; Valkonen, Sami; Korhonen, Ossi; Fernandes, Francisco M.; Yliperttula, Marjo

doi:10.1016/j.jconrel.2021.06.042

Cited by 90 publications

(64 citation statements)

References 127 publications

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“…To obtain an aerogel product of high quality by freeze-drying the critical process parameters, such as freezing rate and shelf temperature, and critical quality attributes, such as residual water content and porosity, must be controlled as we have recently reviewed (Merivaara et al, 2021). Water acts as a plasticizer in the freeze-dried aerogel which can result in collapsing (macro or micro collapse) of the aerogel structures.…”

Section: Introductionmentioning

confidence: 99%

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Near-infrared analysis of nanofibrillated cellulose aerogel manufacturing

Merivaara

Kekkonen

Monola

et al. 2022

International Journal of Pharmaceutics

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Section: Introductionmentioning

confidence: 99%

“…In the future, more sophisticated tools to freeze-dry and to monitor freeze-drying of biomaterials are needed as we discuss in the review article by Merivaara et al (2021) (Merivaara et al, 2021). To answer the need for improved methods, PAT must be applied to the process.…”

Section: Introductionmentioning

confidence: 99%

Near-infrared analysis of nanofibrillated cellulose aerogel manufacturing

Merivaara

Kekkonen

Monola

et al. 2022

International Journal of Pharmaceutics

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“…The presence of vesicles is not surprising since these are shed by C. neoformans during capsule growth (17, 18) and would be retained by the filtration step. In contrast, no vesicles were observed in the lyophilized and reconstituted samples, possibly reflecting collapse of these structures during the drying procedure (19). The lyophilized and reconstituted material does contain dense, rosette-like assemblies, similar to those observed previously for cryptococcal capsular polysaccharide isolations and glycogen (Figure 3B) (6, 20).…”

Section: Resultsmentioning

confidence: 99%

Lyophilization induces alterations in cryptococcal exopolysaccharide resulting in reduced antibody binding

Wear

Hargett

Kelly

et al. 2022

Preprint

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The structural, antigenic, and immunological characterization of microbial polysaccharides requires purification that often involves detergent precipitation and lyophilization. Here we examine physicochemical changes induced by lyophilization on exopolysaccharide (EPS) of the pathogenic fungus Cryptococcus neoformans. Solution 1H NMR reveals significant anomeric signal attenuation following lyophilization of native EPS while 1H ssNMR shows few changes, suggesting diminished molecular motion and consequent broadening of 1H NMR polysaccharide resonances. 13C ssNMR, dynamic light scattering, and transmission electron microscopy show that, while native EPS has rigid molecular characteristics and contains small, loosely packed polysaccharide assemblies, lyophilized and resuspended EPS is disordered and contains larger dense rosette-like aggregates, suggesting that structural water molecules in the interior of the polysaccharide assemblies are removed during extensive lyophilization. Importantly, mAbs to C. neoformans polysaccharide binds the native EPS more strongly than lyophilized EPS. Together, these observations argue for caution when interpreting the biological and immunological attributes of polysaccharides that have been lyophilized to dryness.

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“…Al analizar el comportamiento de la motilidad y viabilidad espermática post descongelación entre los diluyentes a base de YH en sus cuatro diferentes concentraciones, se observa que los porcentajes más bajos son para el nivel 2.25% de YH (D1), al respecto Yodmingkwan et al (2016), en un medio a base de Tris con 2.5% de YH, observan resultados post descongelación muy inferiores a los obtenidos en el presente estudio para motilidad (9.93%) y viabilidad espermática (10.38%), los cuales lo adjudican a la baja concentración de YH utilizada, sin embargo, pudiera estar más relacionado con el bajo porcentaje de glicerol (crioprotector) incorporado (1.4%), que es muy inferior a al recomendado por la literatura internacional (5% -8%) para la crioconservación del semen de esta especie (Bóveda et al, 2020;Sharma and Sood, 2020b). Gojen et al (2016), declaran porcentajes de motilidad y viabilidad más elevados que los nuestros, con un diluyente de composición similar y 2.5% de YH comparado con 5.0%, 7.5% y 10%; esta divergencia de criterios podría explicarse teniendo en cuenta que D1, D2, D3 y D4 fueron liofilizados y se conoce que durante la liofilización, pueden perderse hasta el 50% de las propiedades protectoras del producto (Watson, 1976) debido a la naturaleza altamente energética del proceso, donde se producen cambios de composición en los biomateriales a preservar, lo que hace que las muestras procesadas sean vulnerables a la desnaturalización (Merivaara et al, 2021;Remmele et al, 2012). Analizando estos criterios es posible que concentración de 2.25% de YH (D1) sea insuficiente para brindar protección contra el choque frío durante el periodo de equilibrio Figura 1.…”

Section: Resultados Y Discusiónunclassified

Efecto Del Protector De Membrana Espermática en La Congelabilidad Del Semen Caprino

Duran¹,

Tellez²,

Martinez³

et al. 2022

Trop Subtrop Agroecosyst

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<pre>Background: Prior to freezing goat semen, it is necessary to perform seminal washing by centrifugation to eliminate Phospholipase A, with the consequent loss of elements involved in maintaining sperm functions. Objective: Determine the adequate concentration of egg yolk (YH) for freezing goat semen in a lyophilized diluent based on Tris-glucose and citric acid, without performing seminal washing by centrifugation. Methodology: ninety ejaculates were evaluated with 12 replicates, collected twice a week by means of Artificial Vagina. Volume, motility, concentration, viability and total pathologies were measured. The fit ejaculates were united and divided into five portions, each one received the corresponding diluent: Tris-glucose-Ac. Citrus with YH (2.25%, 3.37%, 4.45% and 5.65%) and the control diluent containing lactose-skimmed milk (DC). The final sperm concentration in the samples was 1.5 x 109 mL</pre>-1. The equilibrium period was carried out at 5°C for 2 h. Subsequently, it was frozen in 0.1 mL tablets in nitrogen vapors, and stored for 7 d in liquid nitrogen, thawed at 37°C and the percentages of motility (30 min, 120 min and 240 min), viability and total pathologies (30 min and 120 min) were determined. The diluents were compared using a Binary Logistic Regression model. Results: YH (4.45%) and DC had the highest probability (P <0.05) of motility at all times. The highest probability (P <0.05) of viability was for YH (4.45%), and the lowest probability (P <0.05) of total pathologies for 4.45% YH and DC, at 30 min and 120 min. Implications: In the freezing of goat semen, it is possible to eliminate the seminal washing process by centrifugation. Conclusion: Goat semen can be frozen in a Tris-based lyophilized extender with 4.45% egg yolk, without performing seminal washing by centrifugation.

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Preservation of biomaterials and cells by freeze-drying: Change of paradigm

Cited by 90 publications

References 127 publications

Near-infrared analysis of nanofibrillated cellulose aerogel manufacturing

Near-infrared analysis of nanofibrillated cellulose aerogel manufacturing

Lyophilization induces alterations in cryptococcal exopolysaccharide resulting in reduced antibody binding

Efecto Del Protector De Membrana Espermática en La Congelabilidad Del Semen Caprino

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