Near-infrared analysis of nanofibrillated cellulose aerogel manufacturing

Merivaara, Arto; Kekkonen, Jere; Monola, Julia; Koivunotko, Elle; Savolainen, Marko; Silvast, Tuomo; Svedström, Kirsi; Díaz, Ana; Holler, Mirko; Korhonen, Ossi; Valkonen, Sami

doi:10.1016/j.ijpharm.2022.121581

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…In addition, by changing the process parameters of freeze-drying, especially by changing the freezing step, different porosities of the material can be obtained [42]. We have previously characterized the porosity of the freeze-dried NFC formulations throughout and similar results were observed in this study evaluated from the SEM micrographs [32,33,43]. The similar porosities observed in the SEM micrographs of this study and our previous studies underline the reproducibility of the freeze-drying cycle and sample preparation.…”

Section: Discussionsupporting

confidence: 81%

“…The mechanical force (stiffness) required by cells of different soft-tissue origins can be easily accessed by controlling the fiber concentration of the NFC hydrogel. Furthermore, freeze-dried NFC hydrogel forms highly porous structures, which can be stored and transported in a dry form at room temperature, and subsequently reconstituted rapidly in one step, as we have recently shown [32,33].…”

Section: Introductionmentioning

confidence: 99%

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Stiffness-Controlled Hydrogels for 3D Cell Culture Models

et al. 2022

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Nanofibrillated cellulose (NFC) hydrogel is a versatile biomaterial suitable, for example, for three-dimensional (3D) cell spheroid culturing, drug delivery, and wound treatment. By freeze-drying NFC hydrogel, highly porous NFC structures can be manufactured. We freeze-dried NFC hydrogel and subsequently reconstituted the samples into a variety of concentrations of NFC fibers, which resulted in different stiffness of the material, i.e., different mechanical cues. After the successful freeze-drying and reconstitution, we showed that freeze-dried NFC hydrogel can be used for one-step 3D cell spheroid culturing of primary mesenchymal stem/stromal cells, prostate cancer cells (PC3), and hepatocellular carcinoma cells (HepG2). No difference was observed in the viability or morphology between the 3D cell spheroids cultured in the freeze-dried and reconstituted NFC hydrogel and fresh NFC hydrogel. Furthermore, the 3D cultured spheroids showed stable metabolic activity and nearly 100% viability. Finally, we applied a convolutional neural network (CNN)-based automatic nuclei segmentation approach to automatically segment individual cells of 3D cultured PC3 and HepG2 spheroids. These results provide an application to culture 3D cell spheroids more readily with the NFC hydrogel and a step towards automatization of 3D cell culturing and analysis.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Stiffness-Controlled Hydrogels for 3D Cell Culture Models

et al. 2022

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“…The absorption peaks near 1500, 1940, 2150, and 2315 nm were relatively strong. The intense absorption peak near 1500 nm was attributed to the first -OH overtone and the first -NH overtone [34]. Additionally, the absorption peak at about 1940 nm was associated with the stretching vibration of the -OH group [35].…”

Section: Spectral Feature Analysismentioning

confidence: 98%

Rapid Measurement of Total Saponins, Mannitol, and Naringenin in Dendrobium officinale by Near-Infrared Spectroscopy and Chemometrics

She,

Huang,

Cao

et al. 2024

Foods

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Dendrobium officinale has drawn increasing attention as a dual-use plant with herbal medicine and food applications. The efficient quality evaluation of D. officinale is essential to ensuring its nutritional and pharmaceutical value. Given that traditional analytical methods are generally time-consuming, expensive, and laborious, this study developed a rapid and efficient approach to assess the quality of D. officinale from different geographical origins by near-infrared (NIR) spectroscopy and chemometrics. Total saponins, mannitol, and naringenin were utilized as quality indicators. Two wavelength selection methods, namely, uninformative variable elimination and competitive adaptive reweighted sampling (CARS), were utilized to enhance the prediction accuracy of the quantification model. Moreover, multiple spectral pretreatment methods were applied for model optimization. Results indicated that the partial least squares (PLS) model constructed based on the wavelengths selected by CARS exhibited superior performance in predicting the contents of the quality indicators. The coefficient of determination (RP2) and root mean square error (RMSEP) in the independent test sets were 0.8949 and 0.1250 g kg−1 for total saponins, 0.9664 and 0.2192 g kg−1 for mannitol, and 0.8570 and 0.003159 g kg−1 for naringenin, respectively. This study revealed that NIR spectroscopy and the CARS-PLS model could be used as a rapid and accurate technique to evaluate the quality of D. officinale.

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“…In recent years, organic polymer aerogels have developed relatively rapidly. Organic polymer aerogel is usually a lightweight, insulating, and good toughness material such as phenolic resin aerogel, 28 polyimide aerogel, 29 cellulose aerogel, 30 polyurethane aerogel 31 . Among them, polyimide aerogel not only has excellent comprehensive performance, but also has the advantages of simple synthesis method, facile operation, and environmental friendly.…”

Section: Introductionmentioning

confidence: 99%

Low cross‐linked polyimide aerogel with imidazole for CO₂ adsorption

Gao

Dong

Zhang

et al. 2022

J of Applied Polymer Sci

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In this study, low cross‐linked mesoporous polyimide aerogels with imidazole were successfully synthesized by co‐polymerization method at room temperature from DMBZ, ABIA, PMDA, and TAB. This synthesis method is milder than previous that of porous material synthesis. The experimental results show that polyimide aerogel with imidazole is a mesoporous material. The thermal decomposition temperatures of obtained polyimide aerogels are all above 450°C, which exhibited outstanding thermal stability. The microscopic morphology varies with the ratio of DMBZ/ABIA monomers. The specific surface area of polyimide aerogel tends to increase first and then decrease with the increase of ABIA content. The largest specific surface area is 240.87 m2/g. However, polyimide aerogel with high specific surface area had not high CO2 adsorption. In contrast, the PAPI‐4 sample with smaller specific surface area had higher CO2 adsorption. The maximum CO2 adsorption capacity is 14.20 cm3/g, which is comparable to those of previously reported porous organic polymers. This suggests that polyimide aerogel can be used as an adsorbent material. The high CO2 adsorptions may be attributed to the abundance of nitrogen‐rich heteroatoms in the polyimide networks.

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Near-infrared analysis of nanofibrillated cellulose aerogel manufacturing

Cited by 7 publications

References 33 publications

Stiffness-Controlled Hydrogels for 3D Cell Culture Models

Stiffness-Controlled Hydrogels for 3D Cell Culture Models

Rapid Measurement of Total Saponins, Mannitol, and Naringenin in Dendrobium officinale by Near-Infrared Spectroscopy and Chemometrics

Low cross‐linked polyimide aerogel with imidazole for CO₂ adsorption

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Near-infrared analysis of nanofibrillated cellulose aerogel manufacturing

Cited by 7 publications

References 33 publications

Stiffness-Controlled Hydrogels for 3D Cell Culture Models

Stiffness-Controlled Hydrogels for 3D Cell Culture Models

Rapid Measurement of Total Saponins, Mannitol, and Naringenin in Dendrobium officinale by Near-Infrared Spectroscopy and Chemometrics

Low cross‐linked polyimide aerogel with imidazole for CO2 adsorption

Contact Info

Product

Resources

About

Low cross‐linked polyimide aerogel with imidazole for CO₂ adsorption