Presentation of BMP‐2 from a Soft Biopolymeric Film Unveils its Activity on Cell Adhesion and Migration

Crouzier, Thomas; Fourel, Laure; Boudou, Thomas; Albigès-Rizo, Corinne; Picart, Catherine

doi:10.1002/adma.201004637

Cited by 123 publications

(181 citation statements)

References 49 publications

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“…165 Matrix binding has been shown to enhance growth factor function over that of the soluble form. 42 In vivo, other cells, such as fibroblasts, may also respond to a chemoattractant by migrating and/or by producing growth factors and chemokines. 58 Testing in animal models showed that lower doses of growth factor resulted in a greater chemotactic response 53 and that chemotaxis in vitro may not correspond to chemotaxis in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…Biomaterials and strategies for chemoattractant delivery are described elsewhere. 31,42 In recent years, a substantial body of work has been published in which various chemoattractants have been tested for MSC recruitment in vitro and in several in vivo models. The objective of this review is to synthesize and evaluate evidence of recruitment efficacy by reported MSC chemoattractants.…”

Section: Introductionmentioning

confidence: 99%

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In SituTissue Regeneration: Chemoattractants for Endogenous Stem Cell Recruitment

Berg-Foels

2014

Tissue Engineering Part B: Reviews

131

111

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Tissue engineering uses cells, signaling molecules, and/or biomaterials to regenerate injured or diseased tissues. Ex vivo expanded mesenchymal stem cells (MSC) have long been a cornerstone of regeneration therapies; however, drawbacks that include altered signaling responses and reduced homing capacity have prompted investigation of regeneration based on endogenous MSC recruitment. Recent successful proof-of-concept studies have further motivated endogenous MSC recruitment-based approaches. Stem cell migration is required for morphogenesis and organogenesis during development and for tissue maintenance and injury repair in adults. A biomimetic approach to in situ tissue regeneration by endogenous MSC requires the orchestration of three main stages: MSC recruitment, MSC differentiation, and neotissue maturation. The first stage must result in recruitment of a sufficient number of MSC, capable of effecting regeneration, to the injured or diseased tissue. One of the challenges for engineering endogenous MSC recruitment is the selection of effective chemoattractant(s). The objective of this review is to synthesize and evaluate evidence of recruitment efficacy by reported chemoattractants, including growth factors, chemokines, and other more recently appreciated MSC chemoattractants. The influence of MSC tissue sources, cell culture methods, and the in vitro and in vivo environments is discussed. This growing body of knowledge will serve as a basis for the rational design of regenerative therapies based on endogenous MSC recruitment. Successful endogenous MSC recruitment is the first step of successful tissue regeneration

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In SituTissue Regeneration: Chemoattractants for Endogenous Stem Cell Recruitment

Berg-Foels

2014

Tissue Engineering Part B: Reviews

131

111

View full text Add to dashboard Cite

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“…The bioactivity of growth factors presented in an immobilized form has also been shown in other studies, indicating that the release of the proteins and their subsequent internalization are not essential for cell stimulation 24,25 . In fact, the covalent immobilization of growth factors allows the sustained stimulation of the target cells while maintaining an effective dosage because of enhanced occupancy rate of different types of receptors at the cell membrane 22,23,[26][27][28] .…”

Section: Discussionmentioning

confidence: 99%

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Pohl

Schwab

Cavalcanti‐Adam

2013

JoVE

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Bone morphogenetic protein 2 (BMP-2) is a growth factor embedded in the extracellular matrix of bone tissue. BMP-2 acts as trigger of mesenchymal cell differentiation into osteoblasts, thus stimulating healing and de novo bone formation. The clinical use of recombinant human BMP-2 (rhBMP-2) in conjunction with scaffolds has raised recent controversies, based on the mode of presentation and the amount to be delivered. The protocol presented here provides a simple and efficient way to deliver BMP-2 for in vitro studies on cells. We describe how to form a self-assembled monolayer consisting of a heterobifunctional linker, and show the subsequent binding step to obtain covalent immobilization of rhBMP-2. With this approach it is possible to achieve a sustained presentation of BMP-2 while maintaining the biological activity of the protein. In fact, the surface immobilization of BMP-2 allows targeted investigations by preventing unspecific adsorption, while reducing the amount of growth factor and, most notably, hindering uncontrolled release from the surface. Both short-and long-term signaling events triggered by BMP-2 are taking place when cells are exposed to surfaces presenting covalently immobilized rhBMP-2, making this approach suitable for in vitro studies on cell responses to BMP-2 stimulation. Video LinkThe video component of this article can be found at

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“…soluble, on a fluid substrate, or immobilized) and if the nature of the receptors plays a role. This issue has been illustrated by the different cellular responses elicited by identical growth factors depending on whether they are soluble or attached to the surrounding matrix (Crouzier et al, 2011).…”

Section: Ecm Sensingmentioning

confidence: 99%

How cells respond to environmental cues – insights from bio-functionalized substrates

Ruprecht

Monzo

Ravasio

et al. 2016

Journal of Cell Science

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Biomimetic materials have long been the (he)art of bioengineering. They usually aim at mimicking in vivo conditions to allow in vitro culture, differentiation and expansion of cells. The past decade has witnessed a considerable amount of progress in soft lithography, bioinspired micro-fabrication and biochemistry, allowing the design of sophisticated and physiologically relevant micro-and nanoenvironments. These systems now provide an exquisite toolbox with which we can control a large set of physicochemical environmental parameters that determine cell behavior. Biofunctionalized surfaces have evolved from simple protein-coated solid surfaces or cellular extracts into nano-textured 3D surfaces with controlled rheological and topographical properties. The mechanobiological molecular processes by which cells interact and sense their environment can now be unambiguously understood down to the single-molecule level. This Commentary highlights recent successful examples where bio-functionalized substrates have contributed in raising and answering new questions in the area of extracellular matrix sensing by cells, cell-cell adhesion and cell migration. The use, the availability, the impact and the challenges of such approaches in the field of biology are discussed.

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Presentation of BMP‐2 from a Soft Biopolymeric Film Unveils its Activity on Cell Adhesion and Migration

Cited by 123 publications

References 49 publications

In SituTissue Regeneration: Chemoattractants for Endogenous Stem Cell Recruitment

In SituTissue Regeneration: Chemoattractants for Endogenous Stem Cell Recruitment

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

How cells respond to environmental cues – insights from bio-functionalized substrates

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