2006
DOI: 10.1128/jcm.01531-06
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Presence of the exoU Gene of Pseudomonas aeruginosa Is Correlated with Cytotoxicity in MDCK Cells but Not with Colonization in BALB/c Mice

Abstract: A total of 141 independent strains of Pseudomonas aeruginosa with different heterogeneities in the exo gene (exoS, exoT, exoU, and exoY) background were examined for their pathogenic roles. Results indicated that the exoU gene is the major contributor to cytotoxicity in Madin-Darby canine kidney cells but is not related to bacterial colonization in mice.

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Cited by 48 publications
(57 citation statements)
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References 13 publications
(16 reference statements)
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“…It shows little pathology in mouse pneumonia 6 , but has significant cytotoxicity in MDCK cells. 10 In contrast, the fourth and most recently described toxin of P. aeruginosa T3SS toxins, exoU shows marked cytotoxic capabilities with remarkably rapid and fulminant cytotoxic effects in many cell types. 7,10 Deletion of exoU severely limits the toxicity of P. aeruginosa strain in lung, and the enzyme has been implicated as an agent associated with septic shock and increased disease severity and mortality in pneumonia.…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…It shows little pathology in mouse pneumonia 6 , but has significant cytotoxicity in MDCK cells. 10 In contrast, the fourth and most recently described toxin of P. aeruginosa T3SS toxins, exoU shows marked cytotoxic capabilities with remarkably rapid and fulminant cytotoxic effects in many cell types. 7,10 Deletion of exoU severely limits the toxicity of P. aeruginosa strain in lung, and the enzyme has been implicated as an agent associated with septic shock and increased disease severity and mortality in pneumonia.…”
mentioning
confidence: 99%
“…10 In contrast, the fourth and most recently described toxin of P. aeruginosa T3SS toxins, exoU shows marked cytotoxic capabilities with remarkably rapid and fulminant cytotoxic effects in many cell types. 7,10 Deletion of exoU severely limits the toxicity of P. aeruginosa strain in lung, and the enzyme has been implicated as an agent associated with septic shock and increased disease severity and mortality in pneumonia. 7,11 Interestingly, the genes encoding P. aeruginosa T3SS toxins are found in some isolates but not in others.…”
mentioning
confidence: 99%
“…However, among these proteins the presence of exoS and exoU is a mutually exclusive event as both the alleles are seldom present in one strain (Lin et al, 2006;Shaver and Hauser, 2004). The strains harboring exoU allele are relatively more cytotoxic and invasive than the alleles having alternative allele, exoS (Lin et al, 2006;Sato and Frank, 2004). In this study all the four new isolates belonged to the exoS allelelic group, therefore, the possibility of antibacterial activity against the mycobacteria due to the product of the cytotoxic gene can be ruled out.…”
Section: Discussionmentioning
confidence: 94%
“…The strains of P. aeruginosa harbor the genes for the four effector proteins; exoT , exoY, exoS and exoU, which are translocated from its type III secretion system, the secretion of these effector proteins determines the cytotoxicity of the strain during infection (Diaz and Hauser, 2010). However, among these proteins the presence of exoS and exoU is a mutually exclusive event as both the alleles are seldom present in one strain (Lin et al, 2006;Shaver and Hauser, 2004). The strains harboring exoU allele are relatively more cytotoxic and invasive than the alleles having alternative allele, exoS (Lin et al, 2006;Sato and Frank, 2004).…”
Section: Discussionmentioning
confidence: 99%
“…The reason for exoS positive strains being associated with lower virulence may be attributed to the poor expression of exoS phenotypes in these isolates and such isolates are phenotypically classified as "neither invasive or nor cytotoxic" (Zhu et al, 2002). Analysis of type III toxin genes in P. aeruginosa MCCB 123 showed the presence of exoS gene, while exoU gene, a major contributor to the potential pathogenesis of P. aeruginosa (Lin H-H et al, 2006) was not detected. exoU has been previously shown to play a major role in mediating a cytotoxic phenotype of P. aeruginosa against lung epithelial cells and HeLa cells (Zaborina et al, 2006).…”
Section: Antibiogrammentioning
confidence: 99%