Presence of pannus-like tissue on osteoarthritic cartilage and its histological character

Shibakawa, Akifumi; Aoki, Hitomi; Masuko, Kayo; Kato, Tomohiro; Tanaka, Mitsuya; Nishioka, Kusuki; Nakamura, Hiroyuki

doi:10.1053/joca.2002.0871

Cited by 77 publications

(78 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pannus has been envisaged as a fibrovascular proliferative tissue that invades articular cartilage at the cartilage-synovium junction. Its formation has been considered to be characteristic of RA, but it has also been reported in OA, which may also be characterized by synovitis (21). It must also be pointed out that the pannus tissue evident microscopically in the present study is likely to differ substantially from the inflamed tissue associated with RA, in which florid destruction of articular cartilage has been documented.…”

Section: Discussionsupporting

confidence: 53%

Microanatomic studies to define predictive factors for the topography of periarticular erosion formation in inflammatory arthritis

McGonagle¹,

Tan²,

Døhn³

et al. 2009

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. The microanatomic basis for formation of erosions in inflammatory arthritis is incompletely understood but is thought to be related to bare areas and the associated cartilage-synovium junction. The purpose of this study was to test the hypothesis that erosion-prone sites are associated with microdamage in macroscopically normal joints.Methods. Histologic evaluation of erosion-prone sites was performed on 20 collateral ligaments (CLs) from the metacarpophalangeal (MCP) and proximal interphalangeal (PIP) joints of 5 normal cadavers. In addition, the MCP joints (n ‫؍‬ 17) and PIP joints (n ‫؍‬ 3) of 20 patients with rheumatoid arthritis (RA) were assessed by computed tomography (CT) to ascertain whether the topography of erosion formation in patients with RA corresponded to the cadaveric findings.Results. Absence of a bare area was noted in cadaveric tissue at the periligamentous erosion-prone regions, especially in the distal MCP joints and both distal and proximal PIP joints. Nevertheless, these sites exhibited soft-tissue pathologic features and bony microdamage/cyst formation. Other significant findings included the presence of pannus without inflammatory changes in the regions in which a bare area was absent, and the replacement of bare area regions with fibrovascular synovial tissue in joints without inflammatory changes. The sites of cadaveric tissue microdamage corresponded to CT-determined erosion formation in the MCP and PIP joints of patients with RA, in whom erosions adjacent to the CLs were more common than dorsal or volar erosions.Conclusion. Periarticular erosion formation may not necessarily depend on the presence of a bare area and has a propensity to occur adjacent to ligaments in which bone microdamage is common. These findings suggest that periligamentous locations prone to microdamage may critically influence the topography of erosion formation in inflammatory arthritis.

show abstract

Section: Discussionsupporting

confidence: 53%

Microanatomic studies to define predictive factors for the topography of periarticular erosion formation in inflammatory arthritis

McGonagle¹,

Tan²,

Døhn³

et al. 2009

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…3). Although OA synovial tissue exhibits histological abnormalities as already reported (17), in the great majority of the cases, the histological changes in OA may be easily distinguished from those observed in RA. In contrast, histological abnormalities were absent in healthy synovium.…”

Section: Histological Analysis Of Clu Protein Expression In the Synoviumsupporting

confidence: 62%

Characterization and Functional Consequences of Underexpression of Clusterin in Rheumatoid Arthritis

Devauchelle

Essabbani

Pinieux

et al. 2006

The Journal of Immunology

View full text Add to dashboard Cite

We previously compared by microarray analysis gene expression in rheumatoid arthritis (RA) and osteoarthritis (OA) tissues. Among the set of genes identified as a molecular signature of RA, clusterin (clu) was one of the most differentially expressed. In the present study we sought to assess the expression and the role of CLU (mRNA and protein) in the affected joints and in cultured fibroblast-like synoviocytes (FLS) and to determine its functional role. Quantitative RT-PCR, Northern blot, in situ hybridization, immunohistochemistry, and Western blot were used to specify and quantify the expression of CLU in ex vivo synovial tissue. In synovial tissue, the protein was predominantly expressed by synoviocytes and it was detected in synovial fluids. Both full-length and spliced isoform CLU mRNA levels of expression were lower in RA tissues compared with OA and healthy synovium. In synovium and in cultured FLS, the overexpression of CLU concerned all protein isoforms in OA whereas in RA, the intracellular forms of the protein were barely detectable. Transgenic overexpression of CLU in RA FLS promoted apoptosis within 24 h. We observed that CLU knockdown with small interfering RNA promoted IL-6 and IL-8 production. CLU interacted with phosphorylated IκBα. Differential expression of CLU by OA and RA FLS appeared to be an intrinsic property of the cells. Expression of intracellular isoforms of CLU is differentially regulated between OA and RA. We propose that in RA joints, high levels of extracellular CLU and low expression of intracellular CLU may enhance NF-κB activation and survival of the synoviocytes.

show abstract

“…Activated synovium can release proteases and cytokines which in turn accelerate the deterioration of adjacent cartilage lesions (22). Our study revealed a difference in LDH levels between the control group and the experimental osteoarthritis group.…”

Section: Discussionmentioning

confidence: 55%

Tartrate-resistant acid phosphatase as a diagnostic factor for arthritis

Seol

Lee²,

Kim³

et al. 2009

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Tartrate-resistant acid phosphatase (TRAP) is highly expressed in osteoclasts and chondroclasts. The present study investigated changes in TRAP activity after chondrocyte death and cartilage damage, and also evaluated the possible use of TRAP as a diagnostic factor in a model of osteoarthritis. We induced experimental osteoarthritis in beagle dogs and separated chondrocytes from articular cartilage using an enzyme probe. Chondrocyte death was induced by proteasome inhibition and TRAIL treatment, and levels of lactate dehydrogenase, reactive oxygen species (ROS), caspase activation and TRAP activity were measured in the chondrocytes and synovial fluid. Proteasome inhibition and TRAIL treatment significantly enhanced chondrocyte death via caspase-8 activation and ROS generation in the primary cultured canine chondrocytes. TRAP activity was highly increased in damaged chondrocytes, but was decreased by blocking chondrocyte death using caspase inhibition or an ROS scavenger. In the synovial fluid of osteoarthritic dogs, TRAP activity as well as caspase activation and ROS levels were higher than those in the normal joint. Our study demonstrated that TRAP is activated by apoptosis and oxidative stress in primary cultured chondrocytes and osteoarthritic joints and also suggests that TRAP may be used as a diagnostic biomarker for detection of cartilage-related diseases, including osteoarthritis.

show abstract

Presence of pannus-like tissue on osteoarthritic cartilage and its histological character

Abstract: Pannus-like tissue exists in advanced OA cartilage, preferentially in the marginal zone. It expressed IL-1beta and MMP3, which strongly suggests that it contributes to cartilage degradation.

Cited by 77 publications

References 19 publications

Microanatomic studies to define predictive factors for the topography of periarticular erosion formation in inflammatory arthritis

Microanatomic studies to define predictive factors for the topography of periarticular erosion formation in inflammatory arthritis

Characterization and Functional Consequences of Underexpression of Clusterin in Rheumatoid Arthritis

Tartrate-resistant acid phosphatase as a diagnostic factor for arthritis

Contact Info

Product

Resources

About