Presence of lettuce big‐vein disease and associated viruses in a subtropical area of Brazil

Colariccio, Addolorata; Chaves, Alexandre Levi Rodrigues; Eiras, Marcelo; Chagas, Carlos; Lenzi, R.; Roggero, P.

doi:10.1111/j.1365-3059.2003.00901.x

Cited by 9 publications

(9 citation statements)

References 3 publications

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“…Both viruses, MiLV and LBVV, are common in mixed infection in lettuce samples from Northern Italy and France . Recently, the same situation was reported in Brazil (Colariccio et al, 2003;Lima Neto et al, 2004). In surveys undertaken between 1998 and 2003, typical big-vein symptoms ( Figure 1A) were observed inthe State of São Paulo that affect different lettuce cultivars, either grown directly in the soil or hydroponically.…”

supporting

confidence: 70%

Detection of Varicosavirus and Ophiovirus in lettuce associated with lettuce big-vein symptoms in Brazil

Colariccio¹,

Chaves²,

Eiras³

et al. 2005

Fitopatol. bras.

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During surveys undertaken from 1998 to 2003 in the major vegetable growing areas of the city of São Paulo green belt, lettuce (Lactuca sativa) and endive (Cichorium endivia) plants were observed, which showed chlorotic thickening of foliar veins, defective growth and, in some cases, failure to form complete heads. Biological and serological [DAS-Enzyme linked immunosorbent assay (Elisa)] tests together with electron microscope observations, revealed the presence of Lettuce big-vein virus and Mirafiori lettuce virus, in these plants both responsible for the lettuce big-vein syndrome.

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supporting

confidence: 70%

Detection of Varicosavirus and Ophiovirus in lettuce associated with lettuce big-vein symptoms in Brazil

Colariccio¹,

Chaves²,

Eiras³

et al. 2005

Fitopatol. bras.

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“…Tissue samples were ground in liquid nitrogen, total nucleic acid was extracted according to the method of Dellaporta et al (1983), and extracts were stored at ¡80°C. Primers CP829F and CP1418R were used for ampliWcation of MLBVV by RT-PCR as described previously (Hayes et al 2006).…”

Section: Mirawori Lettuce Big-vein Virus Detectionmentioning

confidence: 99%

“…Lactuca virosa could be an important source of complete resistance or potential immunity to lettuce big-vein disease (Hayes et al 2006). Big-vein is an economically damaging disease complex of lettuce (Lactuca sativa L.) that occurs in lettuce production regions around the world (Colariccio et al 2003;Fujii et al 2003;Jagger and Chandler 1934;Latham and Jones 2004;Lot et al 2002;Roggero et al 2000;Rosales et al 2004). The disease causing agent is MiraWori lettuce big-vein virus (MLBVV), which is vectored by the soil-borne fungus Olpidium brassicae (Lot et al 2002;Roggero et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation for big-vein symptom expression and resistance to Mirafiori lettuce big-vein virus in Lactuca virosa L., a wild relative of cultivated lettuce

2008

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Lactuca virosa L. is a wild relative of lettuce that is potentially an important source of resistance to big-vein disease, an economically damaging disease of lettuce. IdentiWcation of L. virosa accessions with resistance to MiraWori lettuce big-vein virus (MLBVV), the disease causing agent, may be useful for lettuce breeding. The objectives of this research were to determine the genetic variation for big-vein symptom expression and MLBVV accumulation in diverse L. virosa accessions. Greenhouse testing was conducted to characterize variation for symptom expression 90-100 days after planting (DAP) with 70 L. virosa accessions in unreplicated experiments in 2001 and 2003, and with 10 accessions in an experiment with 3 replications conducted in 2004. In 2005, six replications of seven accessions were evaluated for the percentage of symptomatic plants 120 DAP and 180 DAP in a growth chamber experiment. Reverse transcription-polymerase chain reaction or nucleic acid spot hybridization was used to determine MLBVV presence or absence at each reading date. Genetic variation for symptom expression was conWrmed among the L. virosa accessions, although the majority of tested accessions did not express big-vein symptoms. Symptomless infections were discovered, although accumulation of MLBVV to detectable levels appeared to be a slow process in L. virosa. Genetic variation for the incidence of MLBVV positive plants was identiWed within symptomless accessions, and suggests that symptom expression and MLBVV resistance may be independent factors contributing to big-vein resistance. Regardless, symptomless accessions with low MLBVV incidence were identiWed, and should be useful for breeding new big-vein resistant cultivars.

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“…Nos cinco últimos anos, sintomas da doença conhecida como "big-vein", ou engrossamento das nervuras, vem sendo verificados durante as épocas mais frias do ano no Estado de São Paulo (4). No Estado do Paraná, a doença foi constatada no mesmo ano nas regiões produtoras próximas a Curitiba em plantios convencionais e principalmente nos hidropônicos (10).…”

Section: Frequência De Lettuce Big-vein Associated Virus E Mirafiori unclassified

“…A doença ocorre principalmente em temperaturas inferiores a 20ºC. No Brasil os sintomas têm sido observados principalmente no inverno, quando as temperaturas diurnas variam de 18 a 22ºC e as temperaturas noturnas de 10 a 16ºC (4).…”

Section: Frequência De Lettuce Big-vein Associated Virus E Mirafiori unclassified

Frequência de Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus em Plantas de alface no Estado de São Paulo e transmissão através de extrato vegetal e solo

et al. 2007

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Presence of lettuce big‐vein disease and associated viruses in a subtropical area of Brazil

Cited by 9 publications

References 3 publications

Detection of Varicosavirus and Ophiovirus in lettuce associated with lettuce big-vein symptoms in Brazil

Detection of Varicosavirus and Ophiovirus in lettuce associated with lettuce big-vein symptoms in Brazil

Genetic variation for big-vein symptom expression and resistance to Mirafiori lettuce big-vein virus in Lactuca virosa L., a wild relative of cultivated lettuce

Frequência de Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus em Plantas de alface no Estado de São Paulo e transmissão através de extrato vegetal e solo

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