Presence of human herpesvirus 8 (HHV-8) DNA sequences in patients with lymphoproliferative diseases and chronic blood disorders

Keyvani, Hossein; Niya, Mohammad Hadi Karbalaie; Esghaei, Maryam; Bokharaei‐Salim, Farah; Monavari, Seyed Hamid Reza

doi:10.1016/j.micpath.2017.09.014

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…By considering nuclear antigen sequences (EBNA-2, -3A, -3B, and -3C), EBV can divide in type I and II (Adldinger et al, 1985;Sample et al, 1990). Although, studies on Iranian HL and NHL patients showed the EBV and other herpesviruses such as Kaposi's sarcomaassociated herpesvirus (KSHV) frequency; there were some controversies in the association of EBV infection and its genotypes in these patients (Hesamizadeh et al, 2016;Keyvani et al, 2017). In this preliminary study in Iran, we aimed to detect the presence of EBV genome, EBV genotyping and then viral load quantitation based on EBNA-2 and EBNA-3C genes via molecular techniques, in patients with different hematologic malignancies and chronic lymphoproliferative disorders to introduce their potential risk factors with exposure to EBV infection.…”

Section: Molecular Epidemiology Of Epstein-barr Virus (Ebv) In Patienmentioning

confidence: 99%

Molecular Epidemiology of Epstein-Barr virus (EBV) in Patients with Hematologic Malignancies

Tabibzadeh

Niya

Esghaei

et al. 2020

Asian Pac J Cancer Prev

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Section: Molecular Epidemiology Of Epstein-barr Virus (Ebv) In Patienmentioning

confidence: 99%

Molecular Epidemiology of Epstein-Barr virus (EBV) in Patients with Hematologic Malignancies

Tabibzadeh

Niya

Esghaei

et al. 2020

Asian Pac J Cancer Prev

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“…The hepatitis C virus (HCV), hepatitis B virus (HBV) , HPV, human herpes virus 8 (HHV8) and human T-lymphotropic virus type 1 (HTLV-I) are interesting topics in human cancer investigations that they could be a major cause of different malignancies (Afshar et al, 2018;Karbalaie Niya et al, 2018;Karbalaie Niya et al, 2017b;Keyvani et al, 2017).…”

Section: Research Articlementioning

confidence: 99%

The Molecular Detection of Human Bocavirus (HBoV) in Colorectal Tissue with Malignant and Non-Malignant Lesions

Niya

Ajdarkosh

Tameshkel

et al. 2018

Asian Pac J Cancer Prev

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Background:Colorectal cancer (CRC) as a worldwide human health concern is identified being a multifactorial subject that infection with specific viral particles such as oncogenic viruses is research interest. Human bocavirus (HBoV) as a recent isolated virus has been investigated in many respiratory and enteric diseases but rare studies evaluates it in tissue specimens especially in cancerous sections. The aim of this study was to detect the presence of HBoV genome and its genotyping in CRC patient’s tissue and compare the result with matched healthy control group tissue.Method:in this retrospective case-control study, CRC cases were sporadic and non-familial cancerous while control subjects had healthy or non-malignant lesions in colon tissue. A conventional-PCR performed by specific primers for HBoV VP1 gene. After sequencing of positive PCR products, raw data used for trimming and alignment by bioinformatics software CLC Main Workbench 5 and MEGA5. SPSS v.22 used for statistical calculations.Result:a total of 157 subjects were participated that 66 were diagnosed as CRC cases and 91 were non-CRC colon tissue as control group that matched by the cases. The mean age (y) ± standard deviation of each case and control groups were 59.35±14.48 and 57.21±14.66, respectively. PCR results showed there were 1.3% (2/157) HBoV positive (of each groups one was positive). Sequencing analysis showed all were HBoV-1 genotype.Conclusion:our study showed there are low rate of HBoV genome in Iranian CRC and non-CRC colon tissue. Furthermore, the predominant genotype in our studied subsets were HBoV-1 according to phylogenetic analysis.

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“…After centrifugation, peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood (WB) samples by using Ficoll separation method (Lympholyte H; Cedarlane, Hornby, Canada). [ 19 20 21 ] Briefly, the isolated PBMCs were washed three times with phosphate-buffered saline, and then the cells were resuspended in 200 μL of RNAlater solution (Ambion, Austin, TX). Furthermore, 2.5mL sample of cerebrospinal fluid (CSF) was taken from 164 patients and stored without any preservatives and was directly used to further the extraction procedure.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

A survey on human T-cell lymphotropic virus type 1 (HTLV-1) and xenotropic murine leukemia virus-related virus (XMRV) coinfection in Tehran, Iran

et al. 2018

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Background:Xenotropic murine leukemia virus-related virus (XMRV) is a gamma retrovirus, which has been detected in patients with prostate cancer, chronic fatigue syndrome, and general population with a number of acquired infections such as infection with human T-cell lymphotropic virus (HTLV) and human immunodeficiency virus (HIV). The aim of this study was to determine the HTLV-1 and XMRV coinfection for the first time in Iranian patients who were admitted to the Tehran hospitals.Materials and Methods:Two hundred and ninety one patients suspected with HTLV-1 were referred to the hospitals affiliated to the Iran University of Medical Sciences, Tehran, Iran from April 2012 to October 2016. Genomic deoxyribonucleic acid (DNA)/ribonucleic acid (RNA) from peripheral blood mononuclear cells/cerebrospinal fluids was extracted by High Pure Viral Nucleic Acid Kit (Roche, Germany). After complementary DNA synthesis, conventional reverse transcriptase polymerase chain reaction was used for the detection of HTLV-1 or XMRV-infected patients. Statistical Package for Social Sciences (SPSS) software, version 16 (SPSS, Chicago, IL, USA) was used for statistical analyses.Results:Of the 291 patients suspected of HTLV infection, 123 (42.3%) were male with a mean age of 38±15 years. HTLV-1 RNA was found in 93 (31.9%) specimens comprising 40 men (41.3%) and 53 women (56.9%). Of the 93 patients who were HTLV-1 positive, one sample (1%) was positive for XMRV env gene.Conclusion:These findings suggest that the lack of significant detection of XMRV in patients who were HTLV-1 positive could not be associated with complications of HTLV-1. Although this is a preliminary report from Iranian patients with HTLV-1, further studies are needed to show the actual prevalence of XMRV infection by geographical distribution and various populations.

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Presence of human herpesvirus 8 (HHV-8) DNA sequences in patients with lymphoproliferative diseases and chronic blood disorders

Cited by 5 publications

References 13 publications

Molecular Epidemiology of Epstein-Barr virus (EBV) in Patients with Hematologic Malignancies

Molecular Epidemiology of Epstein-Barr virus (EBV) in Patients with Hematologic Malignancies

The Molecular Detection of Human Bocavirus (HBoV) in Colorectal Tissue with Malignant and Non-Malignant Lesions

A survey on human T-cell lymphotropic virus type 1 (HTLV-1) and xenotropic murine leukemia virus-related virus (XMRV) coinfection in Tehran, Iran

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