Presence of circulating antibodies against gag-gene MuLV proteins in patients with autoimmune connective tissue disorders

Rucheton, Marcel; Graafland, H; Fanton, H.; Ursule, Lisa; Ferrier, Pierre; Larsen, Christian‐Jacques

doi:10.1016/0042-6822(85)90287-9

Cited by 55 publications

(24 citation statements)

References 32 publications

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“…It is interesting to note that Rucheton et al (26) found antibody binding to the p30 gag protein of murine sarcoma virus in patients with SLE. In addition.…”

Section: Resultsmentioning

confidence: 99%

Detection of serum antibodies to retroviral proteins in patients with primary sjögren's syndrome (autoimmune exocrinopathy)

Talal

Dauphinée

Dang

et al. 1990

Arthritis & Rheumatism

215

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Primary Sjögren's syndrome (SS) is considered a benign autoimmune disease; it is characterized by lymphoid infiltration of salivary and lacrimal glands, often accompanied by the presence of serum autoantibodies, particularly anti‐Ro (SS‐A) and anti‐La (SS‐B). There are important immunologic similarities between primary SS and acquired immunodeficiency syndrome. To investigate for a possible immune response to retroviral proteins in primary SS, we performed immunoblotting against human immunodeficiency virus‐1 (HIV‐1) proteins using sera from 47 patients with primary SS. Moderate‐to‐strong reactivity, suggesting the presence of serum antibodies, was found in 14 patients (30%). Of 120 normal subjects, only 1 showed moderate positivity. All 14 positive SS sera reacted against p24 (gag) but failed to react against gp41 or gp120 (env). This response did not reflect hypergammaglobulinemia since immunoglobulin concentrations among the 29 SS patients studied were the same in sera that contained and sera that did not contain anti‐gag reactivity. Two sera also reacted against p17 gag. Four reacted against HIV‐2 core proteins, but none reacted with core proteins of human T lymphotropic virus‐I. Only 1 of the 14 sera reacted against Ro (SS‐A), and 1 other reacted against La (SS‐B). These results identify a subset of SS patients characterized by 1) the presence of serum antibodies to HIV‐1 group‐specific, but not type‐specific, proteins, and 2) the relative absence of anti‐Ro (SS‐A) and anti‐La (SS‐B) autoantibodies. In this latter respect, these SS patients constitute a subpopulation that resembles patients with HIV‐induced SS‐like disease.

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“…It is interesting to note that Rucheton et al (26) found antibody binding to the p30 gag protein of murine sarcoma virus in patients with SLE. In addition.…”

Section: Resultsmentioning

confidence: 99%

Detection of serum antibodies to retroviral proteins in patients with primary sjögren's syndrome (autoimmune exocrinopathy)

Talal

Dauphinée

Dang

et al. 1990

Arthritis & Rheumatism

215

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confidence: 99%

“…From a broader perspective, it is still important to verify the possible presence of such retroviruses in humans. The negative outcome of a broadly targeted serological test (23) not only disproves that humans were XMRV infected but also disproves the occurrence of human infections with the broader group of murine leukemia virus-like retroviruses (MLLVs) (11,56,57).The serological system reported here contains XMRV proteins and peptides homologous to peptides known to react with antibodies to a number of MLLVs, i.e., MuLV, feline leukemia virus (FeLV), and gammaretroviral porcine endogenous retrovirus group C. Moreover, we tried to widen the detection range by the use of degenerate peptides covering known MLLV epitopes. The value of this effort is in (i) the disproving of the occurrence of…”

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confidence: 99%

No Evidence for Xenotropic Murine Leukemia-Related Virus Infection in Sweden Using Internally Controlled Multiepitope Suspension Array Serology

Blomberg

Sjösten

et al. 2012

Clin Vaccine Immunol

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ABSTRACTMany syndromes have a large number of differential diagnoses, a situation which calls for multiplex diagnostic systems. Myalgic encephalomyelitis (ME), also named chronic fatigue syndrome (CFS), is a common disease of unknown etiology. A mouse retrovirus, xenotropic murine leukemia-related virus (XMRV), was found in ME/CFS patients and blood donors, but this was not corroborated. However, the paucity of serological investigations on XMRV in humans prompted us to develop a serological assay which cover many aspects of XMRV antigenicity. It is a novel suspension array method, using a multiplex IgG assay with nine recombinant proteins from theenvandgaggenes of XMRV and 38 peptides based on known epitopes of vertebrate gammaretroviruses. IgG antibodies were sought in 520 blood donors and 85 ME/CFS patients and in positive- and negative-control sera from animals. We found no differences in seroreactivity between blood donors and ME/CFS patients for any of the antigens. This did not support an association between ME/CFS and XMRV infection. The multiplex serological system had several advantages: (i) biotinylated protein G allowed us to run both human and animal sera, which is essential because of a lack of XMRV-positive humans; (ii) a novel quality control was a pan-peptide positive-control rabbit serum; and (iii) synthetic XMRV Gag peptides with degenerate positions covering most of the variation of murine leukemia-like viruses did not give higher background than nondegenerate analogs. The principle may be used for creation of variant tolerant peptide serologies. Thus, our system allows rational large-scale serological assays with built-in quality control.

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“…Immune complexes composed of gp70-anti-gp70 deposit in glomeruli of mice with SLE (16 -19). Murine leukemia viruses (MuLV) have also been implicated in human and murine autoimmune diseases (13,20).…”

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confidence: 99%

Prominent Renal Expression of a Murine Leukemia Retrovirus in Experimental Systemic Lupus Erythematosus

Alexander

Saxena²,

Bao³

et al. 2002

Journal of the American Society of Nephrology

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Abstract.A role for retroviruses in human systemic lupus erythematosus (SLE) and in mouse lupus models such as the New Zealand Black and White mice (NZB/W) strain has been postulated. This study compared the gene profile of nephritic NZB/W kidney with nondiseased NZW controls. The most highly upregulated gene (5.5-fold) hybridized with an expressed sequence tag on a cDNA microarray, which was sequenced and found to correspond with an endogenous murine retrovirus related to the Duplan strain (EDV, L08395). NZB/W kidney contained the full-length 4.2-kb EDV transcript. By 4 wk of age in NZB/W mice, an age preceding renal histologic disease, the EDV transcript was more than threefold increased relative to NZB or NZW control strains. This upregulated expression tended to fall with progression of renal histologic disease. By in situ hybridization, the EDV transcript was highly expressed in tubules of NZB/W mice. There was also upregulated expression of EDV transcript in NZB/W lung and brain, sites of inflammation in this strain, but not in spleen

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Presence of circulating antibodies against gag-gene MuLV proteins in patients with autoimmune connective tissue disorders

Cited by 55 publications

References 32 publications

Detection of serum antibodies to retroviral proteins in patients with primary sjögren's syndrome (autoimmune exocrinopathy)

Detection of serum antibodies to retroviral proteins in patients with primary sjögren's syndrome (autoimmune exocrinopathy)

No Evidence for Xenotropic Murine Leukemia-Related Virus Infection in Sweden Using Internally Controlled Multiepitope Suspension Array Serology

Prominent Renal Expression of a Murine Leukemia Retrovirus in Experimental Systemic Lupus Erythematosus

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