Presence of Cell-Free Fetal DNA in Plasma of Women with Ectopic Pregnancies,

Lázár, Levente; Nagy, Bálint; Bán, Zoltán; Nagy, Gyula Richárd; Papp, Zoltán

doi:10.1373/clinchem.2006.067587

Cited by 20 publications

(12 citation statements)

References 20 publications

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“…After extracting DNA with the silica adsorption method, the amount of cell-free fetal DNA in maternal plasma was determined in patients with male newborns by quantitative real-time PCR analysis of the SRY (sex-determining region Y) gene, as we described previously. 27 Plasma malondialdehyde levels were measured by the thiobarbituric acid-based colorimetric assay. 28 …”

Section: Laboratory Methodsmentioning

confidence: 99%

Circulating angiogenic factors determined by electrochemiluminescence immunoassay in relation to the clinical features and laboratory parameters in women with pre-eclampsia

et al. 2010

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The purpose of this study was to determine whether increased serum soluble fms-like tyrosine kinase-1 (sFlt-1) and decreased placental growth factor (PlGF) levels in pre-eclampsia are related to the clinical features and laboratory parameters of the patients, including markers of inflammation, endothelial activation and injury, oxidative stress and trophoblast debris. A total of 54 pre-eclamptic patients, 58 healthy pregnant and 52 healthy non-pregnant women were involved in this case-control study. Serum sFlt-1 and PlGF levels were measured by electrochemiluminescence immunoassay. Serum levels of sFlt-1 and PlGF were significantly higher in pre-eclamptic patients and healthy pregnant women than in healthy non-pregnant women. In addition, pre-eclamptic patients had significantly higher sFlt-1 levels and significantly lower PlGF concentrations compared with healthy pregnant women. According to the subgroup analyses, sFlt-1 levels were significantly higher in severely pre-eclamptic patients than in those with mild pre-eclampsia, whereas pre-eclamptic patients with fetal growth restriction or preterm onset of the disease had significantly lower PlGF concentrations compared with those without intrauterine growth restriction or with a disease onset at term. In the pre-eclamptic group, there were significant positive correlations between serum sFlt-1 levels and systolic and diastolic blood pressure, serum levels of blood urea nitrogen and creatinine, as well as plasma levels of von Willebrand factor antigen, fibronectin and cell-free fetal DNA. Furthermore, serum PlGF concentrations of pre-eclamptic patients showed significant positive correlations with gestational age at disease onset and delivery, as well as with fetal birth weight, and significant inverse correlations with levels of blood urea nitrogen, creatinine and fibronectin. In conclusion, increased serum sFlt-1 and decreased PlGF levels are associated with blood pressure, renal and endothelial dysfunction, trophoblast deportation, as well as with a shorter duration of pregnancy, fetal growth restriction, the severity and preterm onset of the disease in pre-eclampsia. These findings indicate the central role of an angiogenic imbalance in the pathogenesis of this pregnancy-specific disorder.

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Section: Laboratory Methodsmentioning

confidence: 99%

Circulating angiogenic factors determined by electrochemiluminescence immunoassay in relation to the clinical features and laboratory parameters in women with pre-eclampsia

et al. 2010

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“…The amount of cell-free fetal DNA in maternal plasma was determined in patients with male newborns by quantitative real-time PCR analysis of the sex determining region Y (SRY) gene, as we described previously (47). Briefly, DNA was extracted from 400 mL EDTA anticoagulated plasma with the High Pure PCR Template Preparation Kit (Roche Diagnostics, Mannheim, Germany) according to the manufacturer's protocol.…”

Section: Laboratory Methodsmentioning

confidence: 99%

Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?

Stenczer

Rigó

Prohászka

et al. 2009

Clinical Chemistry and Laboratory Medicine

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Background: It has been previously reported that plasma osteopontin (OPN) concentrations are increased in cardiovascular disorders. The goal of the present study was to determine plasma OPN concentrations in healthy pregnant women and preeclamptic patients, and to investigate their relationship to the clinical characteristics of the study subjects and to markers of inflammation wC-reactive protein (CRP)x, endothelial activation wvon Willebrand factor antigen (VWF:Ag)x or endothelial injury (fibronectin), oxidative stress wmalondialdehyde (MDA)x and trophoblast debris (cellfree fetal DNA). Methods: Forty-four patients with preeclampsia and 44 healthy pregnant women matched for age and gestational age were involved in this case-control study. Plasma OPN concentrations were measured with ELISA. Serum CRP concentrations were determined with an autoanalyzer using the manufacturer's reagents. Plasma VWF:Ag was quantified by ELISA, while plasma fibronectin concentrations were measured by nephelometry. Plasma MDA concentrations were estimated by the thiobarbituric acid-based colorimetric assay. The amount of cell-free fetal DNA in maternal plasma was determined by quantitative real-time PCR analysis of the sexdetermining region Y (SRY) gene. For statistical analyses, non-parametric methods were applied.

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“…Foetal DNA escaping into the maternal circulation has also been analysed with more positive results. Lazar et al (2006) examined cell-free foetal DNA, looking at the Sry gene, in blood samples from 58 women. They identified the Sry gene in 15 women who had a tubal ectopic pregnancy and 14 women with an intrauterine pregnancy, and its concentration was significantly higher in those with a tubal ectopic pregnancy (P!0.001).…”

Section: Activin Amentioning

confidence: 99%

“…Using a cut-off of O80 GE/ml, this test was able to differentiate a tubal ectopic from an intrauterine pregnancy with sensitivity of 84%, a specificity of 76%, a positive predictive value (PPV) of 83% and a negative predictive value (NPV) of 83% (Lazar et al 2006). This technology at present is lengthy and complex and cannot be used when the conceptus is female, so its utility as a clinically useful serum biomarker is very limited.…”

Section: Activin Amentioning

confidence: 99%

Serum biomarkers of tubal ectopic pregnancy: current candidates and future possibilities

Cartwright¹,

Duncan²,

Critchley³

et al. 2009

Reproduction

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Ectopic pregnancy remains a considerable cause of maternal morbidity and mortality worldwide. Currently, it is diagnosed using a combination of transvaginal ultrasound and serial serum b-human chorionic gonadotrophin levels. Diagnosis is often delayed and these tests are time-consuming and costly, both psychologically to the patient and financially to health services. The development of a biomarker that can differentiate a tubal ectopic from an intrauterine implantation is therefore important. In the pre-genomic era, a one-by-one scientific approach has revealed over 20 candidate biomarkers that could be used as a test to diagnose ectopic pregnancy although at present their clinical utility is very limited. These biomarkers cluster into themes: markers of abnormal embryo/trophoblast growth, markers of abnormal corpus luteum function, markers of a growing pregnancy in the Fallopian tube, markers of inflammation and peritoneal irritation, and uterine markers of normal implantation. It is likely that this thematic approach will facilitate the identification of newer biomarkers using microarray technology and inform the development of investigative paradigms using multiple markers at the time of presentation.

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Presence of Cell-Free Fetal DNA in Plasma of Women with Ectopic Pregnancies,

Cited by 20 publications

References 20 publications

Circulating angiogenic factors determined by electrochemiluminescence immunoassay in relation to the clinical features and laboratory parameters in women with pre-eclampsia

Circulating angiogenic factors determined by electrochemiluminescence immunoassay in relation to the clinical features and laboratory parameters in women with pre-eclampsia

Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?

Serum biomarkers of tubal ectopic pregnancy: current candidates and future possibilities

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