Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?

Stenczer, Balázs; Rigó, János; Prohászka, Zoltán; Derzsy, Zoltán; Lázár, Levente; Makó, Veronika; Cervenak, László; Balogh, Krisztián; Mézes, Miklós; Karádi, István; Molvarec, Attila

doi:10.1515/cclm.2010.042

Cited by 15 publications

(15 citation statements)

References 56 publications

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“…Improved capillary penetrability secondary to endothelial destruction seems to be partly responsible for this result [13]. There were A significant increase in mean levels of Inhibin B and Osteopontin, in group A [pregnant women with preeclampsia] comparing to group B [healthy pregnant women] (P<0.01) as shown in Table (2). A significant increase in mean levels of Osteopontin in the present study agreement with Stenczer B et al [14] while another study showed no significant difference in plasma Osteopontin concentrations between the preeclamptic and the control group [15]. The extravillous trophoblasts of the human placenta also express Osteopontin, which regulates the invasiveness of these cells [16] Osteopontin may be released from the vessel wall into the peripheral circulation in cases of extensive endothelial injury, and may be used as a marker of endothelial injury in patient with preeclampsia.…”

Section: Resultssupporting

confidence: 93%

Determination of Plasma Osteopontin and Serum Inhibin B in Pregnant Women with Preeclampsia

Mehde

Mehdi

Mustafa

2014

JNUS

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Pre-eclampsia is a multisystem disorder of pregnancy, which complicates 3%-5% of pregnancies in the world .The present study deals with estimation of levels of Osteopontin, Inhibin B, protein, albumin, globulin and Alb./Glb. ratio in forty five Pregnant Women with Preeclampsia in comparison with thirty healthy Pregnant Women. In Pregnant Women with Preeclampsia, the level of Osteopontin and Inhibin B were significantly higher as compared to the normal subjects [p<0.01]. In Pregnant Women with Preeclampsia the levels of S. calcium S.Protein and albumin were significantly decreased as compared to the normal subjects [p<0.05, p<0.05and p<0.01] respectively. Based on findings of the present study, it can be concluded that Patients with Preeclampsia had significantly higher serum inhibin B and Osteopontin concentrations compared to healthy pregnant women. In addition, the current study could not determine any links between Osteopontin levels and other biochemical markers in this study.

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Section: Resultssupporting

confidence: 93%

Determination of Plasma Osteopontin and Serum Inhibin B in Pregnant Women with Preeclampsia

Mehde

Mehdi

Mustafa

2014

JNUS

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“…Osteopontin (OPN) is involved in adhesion and signal transduction at the uterine-placental interface during implantation and placentation [73]. Importantly, OPN acts as a pro-inflammatory Th1 type cytokine in vivo [74] and preeclamptic patients with extensive endothelial injury are characterized by increased plasma levels of OPN [75]. Thus, the increased OPN production by PE-PDMSCs that we reported could cause and/or further contribute to the aberrant placental inflammation underlying preeclampsia.…”

Section: Discussionmentioning

confidence: 63%

Pro-Inflammatory Profile of Preeclamptic Placental Mesenchymal Stromal Cells: New Insights into the Etiopathogenesis of Preeclampsia

et al. 2013

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The objective of the present study was to evaluate whether placental mesenchymal stromal cells (PDMSCs) derived from normal and preeclamptic (PE) chorionic villous tissue presented differences in their cytokines expression profiles. Moreover, we investigated the effects of conditioned media from normal and PE-PDMSCs on the expression of pro-inflammatory Macrophage migration Inhibitory Factor (MIF), Vascular Endothelial Growth Factor (VEGF), soluble FMS-like tyrosine kinase-1 (sFlt-1) and free β-human Chorionic Gonadotropin (βhCG) by normal term villous explants. This information will help to understand whether anomalies in PE-PDMSCs could cause or contribute to the anomalies typical of preeclampsia.MethodsChorionic villous PDMSCs were isolated from severe preeclamptic (n = 12) and physiological control term (n = 12) placentae. Control and PE-PDMSCs’s cytokines expression profiles were determined by Cytokine Array. Control and PE-PDMSCs were plated for 72 h and conditioned media (CM) was collected. Physiological villous explants (n = 48) were treated with control or PE-PDMSCs CM for 72 h and processed for mRNA and protein isolation. MIF, VEGF and sFlt-1 mRNA and protein expression were analyzed by Real Time PCR and Western Blot respectively. Free βhCG was assessed by immunofluorescent.ResultsCytokine array showed increased release of pro-inflammatory cytokines by PE relative to control PDMSCs. Physiological explants treated with PE-PDMSCs CM showed significantly increased MIF and sFlt-1 expression relative to untreated and control PDMSCs CM explants. Interestingly, both control and PE-PDMSCs media induced VEGF mRNA increase while only normal PDMSCs media promoted VEGF protein accumulation. PE-PDMSCs CM explants released significantly increased amounts of free βhCG relative to normal PDMSCs CM ones.ConclusionsHerein, we reported elevated production of pro-inflammatory cytokines by PE-PDMSCs. Importantly, PE PDMSCs induced a PE-like phenotype in physiological villous explants. Our data clearly depict chorionic mesenchymal stromal cells as central players in placental physiopathology, thus opening to new intriguing perspectives for the treatment of human placental-related disorders as preeclampsia.

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“…Given that late pregnancy is an insulin-resistant state and that women with gestational diabetes are known to have even increased insulin resistance, osteopontin might be implicated in the development of insulin resistance during pregnancy per se and/or the aggravation of insulin resistance in gestational diabetes. Of note, osteopontin was reported to be elevated in women with preeclampsia [10] and like gestational diabetes, also preeclampsia has been linked to increased insulin resistance during pregnancy [11].…”

Section: Introductionmentioning

confidence: 99%

CTX (Crosslaps) Rather than Osteopontin Is Associated with Disturbed Glucose Metabolism in Gestational Diabetes

et al. 2012

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Objective Reciprocal interaction between bone and glucose metabolism might play a pivotal role in the development of type 2 diabetes. We recently demonstrated that osteocalcin is increased in women with gestational diabetes (GDM) compared to healthy pregnant women and related to enhanced insulin secretion. Here, we aimed to investigate the role of the bone resorption marker CTX and osteopontin (OPN), a key molecule in subclinical inflammation underlying insulin resistance, in gestational diabetes. Methods Insulin sensitivity and secretion (derived from OGTT) as well as CTX and osteopontin were investigated in 26 GDM and 52 women with normal glucose tolerance during pregnancy [CON] between 24th and 28th gestational weeks; 24 women also underwent postpartum examination. Results CTX was significantly higher in GDM compared to CON (0.44±0.20 vs.0.28±0.12 ng/ml, p<.0001) and positively correlated with osteocalcin (R = 0.64, p<.0001) and parameters of insulin secretion. Osteopontin plasma concentrations were decreased in GDM compared to CON (28.81±22.12 vs.37.68±19.63 ng/ml, p = 0.04), and did not show any relation to insulin secretion or sensitivity, but were significantly correlated with CRP (R = 0.3, p<0.007) and liver enzymes. Twelve weeks after delivery CTX and OPN were increased compared to pregnancy (both p<.0001) and did not differ between GDM and CON. Conclusion Our findings support the idea of a tight regulation between bone and glucose metabolism, and suggest, that less curbed CTX during pregnancy might be involved in osteocalcin-mediated amelioration of insulin secretion in GDM. On the other hand, osteopontin was unrelated to insulin resistance in GDM, but associated with inflammatory markers and liver enzymes in all women.

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Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?

Cited by 15 publications

References 56 publications

Determination of Plasma Osteopontin and Serum Inhibin B in Pregnant Women with Preeclampsia

Determination of Plasma Osteopontin and Serum Inhibin B in Pregnant Women with Preeclampsia

Pro-Inflammatory Profile of Preeclamptic Placental Mesenchymal Stromal Cells: New Insights into the Etiopathogenesis of Preeclampsia

CTX (Crosslaps) Rather than Osteopontin Is Associated with Disturbed Glucose Metabolism in Gestational Diabetes

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