Presence of caprine arthritis–encephalitis virus (CAEV) proviral DNA in genital tract tissues of superovulated dairy goat does

Fieni, F.; Rowe, Joan D; Hoosear, K. Van; Burucoa, Christophe; Oppenheim, S. M.; Anderson, G. B.; Murray, James D.; Bondurant, Robert

doi:10.1016/s0093-691x(02)01194-9

Cited by 45 publications

(22 citation statements)

References 23 publications

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“…However, viral particles may eventually pass through to the fetus, considering that they may be found in the reproductive tract (ANDRIOLI et al, 2002;CAVALCANTE et al, 2013). ANDRIOLI et al (2002) made viral isolation followed by nested-PCR and found that CAEV was present in the uterine fluid of positive goat nannies, corroborating with the findings of FIENI et al (2003), who also identified the presence of this virus in embryo washings. CAVALCANTE et al (2013) observed with reverse transcription polimerase chain reaction (RT-PCR) that six goats presented free viral particles in the uterine fluid, demonstrating the potential to transmit this pathogen, which explains the production of antibodies detected by WB in the newborn goat kids in this study.…”

Section: Resultsmentioning

confidence: 66%

“…Pro-viral DNA was also detected in oviduct and ovarian (FIENI et al, 2003) cells from cumulus oophorus (ALI AL AHMAD et al, 2005) in the ovarian cortex, pre-antral follicles (SILVA, 2006), in semen and vaginal duct, which provide the necessary conditions for a possible ingestion of contaminated maternal fluids by the offspring (EAST et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of caprine arthritis-encephalitis virus transmission in newborn goat kids

et al. 2018

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ABSTRACT:Caprine arthritis encephalitis causes considerable losses in goat production. The main form of the caprine arthritis encephalitis virus transmission is through the ingestion of colostrum or milk from infected females. However, some transmissions cannot be explained in this manner. Therefore, this study aimed to evaluate transplacental transmission of caprine arthritis encephalitis virus. Blood samples were collected from 283 newborn kids of Anglo-Nubian and Saanen breeds born from seropositive and seronegative goats. Samples were collected immediately after birth and analyzed with agarose gel immunodiffusion and western blot. All samples were negative in the agarose gel immunodiffusion. However, the western blot test demonstrated that four kids were born positive for caprine arthritis encephalitis virus. This result indicates that although in a low frequency (1.4%), there is a possibility of transplacental transmission of small ruminant lentivirus.KEYWORDS: small ruminant lentivirus; agarose gel immunodiffusion; western blot. RESUMO:A artrite encefalite caprina causa perdas considerá-veis para a produção caprina. A principal forma de transmissão do vírus da artrite encefalite caprina é a ingestão de colostro ou leite de fêmeas infectadas. No entanto, algumas transmissões não podem ser explicadas por esta via. Dessa forma, este estudo teve como objetivo avaliar a transmissão do vírus da artrite encefalite caprina por via transplacentária (vertical). Foram realizadas coletas de sangue em 283 crias recém-nascidas das raças Anglo-Nubiana e Saanen, provenientes de progenitores soropositivos e soronegativos. As amostras foram coletadas logo após o nascimento e analisadas pelas técnicas de imunodifusão em gel de agarose e western blot. No teste de imunodifusão em gel de agarose, nenhum cabrito foi detectado reagente. Porém, no teste de western blot, quatro cabritos nasceram soropositivos. Esse resultado indica que, apesar de baixa frequência (1,4%), existe a possibilidade de transmissão via transplacentária do lentivírus de pequenos ruminantes.PALAVRAS-CHAVE: lentivírus de pequenos ruminantes; imunodifusão em gel de agarose; western blot.

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Section: Resultsmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Evaluation of caprine arthritis-encephalitis virus transmission in newborn goat kids

et al. 2018

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“…The placenta has also been considered in the context of the intrauterine transmission of SRLVs [2,50]. A recent study has demonstrated the presence of CAEV-infected cells in the goat genital tract which suggests the potential role of this tract for vertical transmission of CAEV from doe to embryo or fetus [49]. Data on the virus content of ovocytes are not available.…”

Section: The Role Of Derived Products In Viral Transmissionmentioning

confidence: 99%

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

et al. 2004

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-Small ruminant lentiviruses (SRLV = maedi-visna in sheep and caprine arthritis encephalitis in goats) are distributed throughout most countries of the world, particularly Europe. Laboratories from 16 European countries established collaborations within the framework of a COST (CO-operation in the field of Scientific and Technical Research) action sponsored by the European Union in order to (i) better organize their research programmes on SRLVs and (ii) to coordinate efforts to combat these two diseases. After five years, a consensus conference -the first one in the veterinary medicine field -concluded the work of this network of laboratories by reviewing the present position and discussing three important questions in the field of SRLVs: routes of transmission, consequences of infection and potential role of eradication programmes at either a European or local level, according to the situation in each country or region. This paper brings together existing information regarding these questions and identifies areas for future research.Maedi-visna / caprine arthritis-encephalitis virus / lentivirus / small ruminants / European consensus conference

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“…L'analyse des différents tissus de l'appareil génital de chèvres donneuses d'embryons a permis de mettre en évidence de l'ADN proviral de CAEV dans les ovaires, les cellules de la corona radiata, l'oviducte et l'utérus (Fieni et al, 2003 ;Ali Al Ahmad et al, 2005) mais sans précision du type cellulaire infecté (monocytes/macrophages ou cellules épithéliales).…”

Section: Risques Liés Au Transfert Embryonnaireunclassified

Evaluation du risque de transmission du virus de l’arthrite encéphalite caprine (CAEV) par les biotechnologies de la reproduction

Fieni¹

2014

bavf

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Evaluation of the risk of CAEV (caprine arthritis encephalitis virus) transmission via assisted reproduction techniques Caprine Arthritis-Encephalitis Virus (CAEV) has been identified in the tissues of male and female genital tract, in the semen, in uterine and vaginal secretions, and in embryo flushing medias. So a health risk is associated with artificial insemination (AI), In vitro fecundation (IVF) and embryo transfer (ET). However, it has been demonstrated that sperm fraction, oocytes free of granulosa cells, embryo with intact zona pellicida and after ten washing, take off from naturally infected goats are free of CAEV. So with the respect of definite process, IA, IVF and ET are associated with low level CAEV transmission risk and TE can be use as preventive health measure.

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Presence of caprine arthritis–encephalitis virus (CAEV) proviral DNA in genital tract tissues of superovulated dairy goat does

Cited by 45 publications

References 23 publications

Evaluation of caprine arthritis-encephalitis virus transmission in newborn goat kids

Evaluation of caprine arthritis-encephalitis virus transmission in newborn goat kids

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

Evaluation du risque de transmission du virus de l’arthrite encéphalite caprine (CAEV) par les biotechnologies de la reproduction

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